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    DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS
    2.
    发明申请
    DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS 有权
    检测系统和高灵敏度荧光测定方法

    公开(公告)号：US20110312105A1

    公开(公告)日：2011-12-22

    申请号：US13224174

    申请日：2011-09-01

    申请人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

    发明人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

    IPC分类号： G01N21/64 ,  C07K14/36 ,  C07K16/00

    CPC分类号： G01N33/54366 ,  C08B37/006 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2201/06113

    摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.

    摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。

    Methods and kits for the inhibition of hyperplasia in vascular fistulas and grafts
    3.
    发明授权
    Methods and kits for the inhibition of hyperplasia in vascular fistulas and grafts 失效
    用于抑制血管瘘和移植物增生的方法和试剂盒

    公开(公告)号：US06755853B2

    公开(公告)日：2004-06-29

    申请号：US10010411

    申请日：2001-12-05

    申请人： John R. McKenzie ,  Robert F. Zuk ,  Axel F. Brisken ,  Paul D. Corl

    发明人： John R. McKenzie ,  Robert F. Zuk ,  Axel F. Brisken ,  Paul D. Corl

    IPC分类号： A61F206

    CPC分类号： A61F2/02 ,  A61F2/06

    摘要： A method for inhibiting hyperplasia at an anastomotic junction of a vascular fistula or vascular graft comprises exposing the anastomotic junction to vibrational energy at a mechanical index and for a time sufficient to inhibit hyperplasia. Kits are described comprising the catheter or a probe suitable for performing the methods together with instructions for use setting forth the methods. The methods and kits are particularly suitable for treating anastomotic junctions formed to provide vascular access for hemodialysis, hemofiltration, and the like. The methods and kits are also suitable for treating other vascular grafts, such as those formed during cardiac bypass graft surgery, vascular repair, and the like.

    摘要翻译： 用于抑制血管瘘或血管移植物的吻合接合处的增生的方法包括以机械指数将吻合接头暴露于振动能量并足以抑制增生的时间。 描述的试剂盒包括适于执行方法的导管或探针以及阐述方法的使用说明书。 所述方法和试剂盒特别适用于治疗形成的吻合口以提供用于血液透析，血液滤过等的血管通路。 方法和试剂盒也适用于治疗其它血管移植物，例如在心脏旁路移植手术期间形成的血管移植物，血管修复等。

    Method and kit for pyridinium crosslink assay
    5.
    发明授权
    Method and kit for pyridinium crosslink assay 失效
    吡啶交联试验方法和试剂盒

    公开(公告)号：US5620861A

    公开(公告)日：1997-04-15

    申请号：US484212

    申请日：1995-06-07

    申请人： Mary J. Cerelli ,  Hsin-Shan J. Ju ,  Robert F. Zuk

    发明人： Mary J. Cerelli ,  Hsin-Shan J. Ju ,  Robert F. Zuk

    IPC分类号： A61C19/06 ,  C07K16/18 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68 ,  G01N33/543

    CPC分类号： C07K16/18 ,  G01N33/5308 ,  G01N33/57407 ,  G01N33/6887 ,  A61C19/063 ,  G01N2800/108 ,  Y10S435/81 ,  Y10S435/975

    摘要： A method of assaying bone collagen degradation activity in a human subject. In the method, a human urine sample is reacted with an antibody which (i) is capable of reacting immunospecifically with pyridinium crosslinks selected from the group consisting of native free pyridinoline and native free deoxypyridinoline, and (ii) has a ratio of reactivity toward the selected pyridinium crosslinks and urinary pyridinium peptides larger than 1,000 daltons in molecular weight, of greater than about 5:1. An immunocomplex forms between the antibody reagent and the selected pyridinium crosslinks which are present in the sample, and the amount of immunocomplex is measured. Also disclosed are antibody reagents and kits which can be used in the method.

    摘要翻译： 测定人类受试者骨胶原降解活性的方法。 在该方法中，人尿样品与抗体反应，（i）能够与选自天然游离吡啶啉和天然游离的脱氧吡啶啉的吡啶鎓交联物进行免疫特异性反应，和（ii）具有与 选择的吡啶鎓交联剂和大于1000道尔顿的尿吡啶肽分子量大于约5：1。 在抗体试剂和存在于样品中的所选择的吡啶鎓交联之间形成免疫复合物，并测量免疫复合物的量。 还公开了可用于该方法的抗体试剂和试剂盒。

    Immunoassay cuvettes
    6.
    发明授权
    Immunoassay cuvettes 有权
    免疫测定池

    公开(公告)号：US08304255B1

    公开(公告)日：2012-11-06

    申请号：US12369564

    申请日：2009-02-11

    申请人： Robert F. Zuk ,  Hong Tan

    发明人： Robert F. Zuk ,  Hong Tan

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54386

    摘要： The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound.

    摘要翻译： 本发明涉及包含扩散结合和非扩散结合试剂进行免疫测定的免疫测定试管。 反应和检测在免疫测定试管中进行。 免疫测定试管包括透明的前壁，后壁，侧壁，底部和顶部开口。 试管的后壁具有由无孔材料制成的基本平坦的表面，并且包括具有非扩散结合的试剂的捕获区和具有扩散结合的试剂的信号试剂区。

    Method for plasma separation and measurement
    8.
    发明授权
    Method for plasma separation and measurement 失效
    血浆分离和测量方法

    公开(公告)号：US5798272A

    公开(公告)日：1998-08-25

    申请号：US761909

    申请日：1996-12-09

    申请人： Michael P. Allen ,  Robert F. Zuk ,  Lawrence M. Ensler

    发明人： Michael P. Allen ,  Robert F. Zuk ,  Lawrence M. Ensler

    IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N33/49

    CPC分类号： G01N33/491 ,  G01N1/2813 ,  G01N35/1095 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The system for transferring plasma to a test substrate comprises an applicator and the test substrate. The applicator includes a blood reservoir and a filter element which separates cellular components from the blood as plasma is passed to the test substrate. The test substrate includes an absorptive element which receives the plasma from the applicator. In one embodiment, measured amounts of plasma are transferred when the absorptive element becomes saturated, thus stopping the flow of plasma. In another embodiment, excess blood and cellular components are removed from the measured plasma by disengaging the applicator from the test substrate. In another embodiment, plasma transferred to the absorptive element is eluted from the absorptive element by subsequent application of an elution medium.

    摘要翻译： 用于将等离子体转移到测试基板的系统包括施加器和测试基板。 施用器包括血液储存器和过滤器元件，当等离子体被传递到测试基底时，其将细胞组分与血液分离。 测试基板包括从施加器接收等离子体的吸收元件。 在一个实施例中，当吸收元件饱和时转移等离子体的测量量，从而停止等离子体的流动。 在另一个实施方案中，通过使施用器与测试底物脱离，从测量的等离子体中除去多余的血液和细胞成分。 在另一个实施方案中，转移到吸收元件的血浆通过随后施加洗脱介质从吸收元件洗脱。

    System and method for plasma separation and measurement
    9.
    发明授权
    System and method for plasma separation and measurement 失效
    血浆分离和测量的系统和方法

    公开(公告)号：US5589399A

    公开(公告)日：1996-12-31

    申请号：US326974

    申请日：1994-10-21

    申请人： Michael P. Allen ,  Robert F. Zuk ,  Lawrence M. Ensler

    发明人： Michael P. Allen ,  Robert F. Zuk ,  Lawrence M. Ensler

    IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N33/49 ,  G01N21/01

    CPC分类号： G01N33/491 ,  G01N1/2813 ,  G01N35/1095 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The system for transferring plasma to a test substrate comprises an applicator and the test substrate. The applicator includes a blood reservoir and a filter element which separates cellular components from the blood as plasma is passed to the test substrate. The test substrate includes an absorptive element which receives the plasma from the applicator. In one embodiment, measured amounts of plasma are transferred when the absorptive element becomes saturated, thus stopping the flow of plasma. In another embodiment, excess blood and cellular components are removed from the measured plasma by disengaging the applicator from the test substrate. In another embodiment, plasma transferred to the absorptive element is eluted from the absorptive element by subsequent application of an elution medium.

    摘要翻译： 用于将等离子体转移到测试基板的系统包括施加器和测试基板。 施用器包括血液储存器和过滤器元件，当等离子体被传递到测试基底时，其将细胞组分与血液分离。 测试基板包括从施加器接收等离子体的吸收元件。 在一个实施例中，当吸收元件饱和时转移等离子体的测量量，从而停止等离子体的流动。 在另一个实施方案中，通过使施用器与测试底物脱离，从测量的等离子体中除去多余的血液和细胞成分。 在另一个实施方案中，转移到吸收元件的血浆通过随后施加洗脱介质从吸收元件洗脱。

    Measuring with zero volume cell
    10.
    发明授权
    Measuring with zero volume cell 失效
    用零容量单元进行测量

    公开(公告)号：US4911794A

    公开(公告)日：1990-03-27

    申请号：US65418

    申请日：1987-06-18

    申请人： John W. Parce ,  Robert F. Zuk

    发明人： John W. Parce ,  Robert F. Zuk

    IPC分类号： C12Q1/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/414 ,  G01N27/416

    CPC分类号： G01N27/3271 ,  Y10S435/817 ,  Y10S436/806

    摘要： Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

    摘要翻译： 描述了用于确定疑似含有分析物的样品中分析物的存在的方法和装置。 该方法包括在相对较大体积的测定培养基的存在下使能够通过半导体电极检测的可测定元件的浓缩物与电极接触。 浓缩物中可测量的元素以与样品中存在的分析物的量相关的量存在。 然后减少与浓缩物扩散连通的介质的体积，并且通过可测定元件在半导体电极上具有的作用来检测可确定元件。