公开(公告)号：US20160186179A1

公开(公告)日：2016-06-30

申请号：US15010837

申请日：2016-01-29

Applicant: Larry J. Smith

Inventor： Larry J. Smith

IPC: C12N15/113

CPC classification number: C12N15/1135 ,  A61K31/712 ,  A61K31/7125 ,  A61K31/713 ,  C12N15/113 ,  C12N15/1136 ,  C12N15/1137 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3513 ,  C12N2310/51 ,  C12N2320/31 ,  C12N2320/35 ,  C12N2320/51 ,  C12N2310/3521 ,  A61K2300/00

Abstract: Compositions and method for down modulating target gene expression which RNA interference, as well as methods for administering said compositions are disclosed. The method comprises administering a first oligonucleotide strand to a cell, incubating the cells for a time period suitable for uptake of the first oligo nucleotide strand prior to administration of a second oligonucleotide strand, wherein the first strand and the second strand form an intracellular duplex which is effective to catalyze degradation of gene target mRNA or inhibit translation of said mRNA.

Abstract translation: 公开了用于降低靶基因表达的组合物和方法，其中RNA干扰以及用于施用所述组合物的方法。 所述方法包括向细胞施用第一寡核苷酸链，在施用第二寡核苷酸链之前将细胞温育适合摄取第一寡核苷酸链的一段时间，其中第一链和第二链形成细胞内双链体， 有效地催化基因靶mRNA的降解或抑制所述mRNA的翻译。

公开(公告)号：US20150291957A1

公开(公告)日：2015-10-15

申请号：US14438857

申请日：2013-10-28

Applicant: Larry J. SMITH

Inventor： Larry J. Smith

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/32 ,  C12N2320/50 ,  C12N2320/51 ,  C12N2320/52

Abstract: Compositions and methods for down modulating expression of target nucleic acids are disclosed. This invention relates to the fields of medicine, drug development and modulation of target nucleic acid expression. More specifically, the invention provides compositions and methods of use thereof that facilitate the modulation of target nucleic acid expression using novel oligonucleotide based drugs that act through an inhibitory RNA (RNAi) mechanism of action.

Abstract translation: 公开了用于降低靶核酸表达的组合物和方法。 本发明涉及药物领域，药物开发和靶核酸表达的调控。 更具体地，本发明提供了使用通过抑制性RNA（RNAi）作用机制起作用的新型基于寡核苷酸的药物来调节靶核酸表达的组合物及其使用方法。

公开(公告)号：US20100144840A1

公开(公告)日：2010-06-10

申请号：US12636452

申请日：2009-12-11

Applicant: Dayton T. Reardan ,  Larry J. Smith ,  Robert E. Klem

Inventor： Dayton T. Reardan ,  Larry J. Smith ,  Robert E. Klem

IPC: A61K31/7088 ,  A61K31/713

CPC classification number: C07K16/1063 ,  A61K31/136 ,  A61K31/70 ,  A61K31/7088 ,  A61K31/713 ,  A61K39/39558 ,  A61K39/39575 ,  A61K2039/505 ,  C07K16/32 ,  C07K2317/34 ,  C12N9/0002 ,  A61K2300/00

Abstract: Covalently reactive antigen analogs are disclosed herein. The antigens of the invention may be used to stimulate production of catalytic antibodies specific for predetermined antigens associated with particular medical disorders. The antigen analogs may also be used to permanently inactivate endogenously produced catalytic antibodies produced in certain autoimmune diseases as well as in certain lymphoproliferative disorders. Also provided are methods for modulating the efficacy of nucleic acid based therapeutics.

Abstract translation: 本文公开了共价反应性抗原类似物。 本发明的抗原可用于刺激对与特定医学病症相关的预定抗原特异性的催化抗体的产生。 抗原类似物也可用于永久灭活在某些自身免疫性疾病以及某些淋巴组织增生性疾病中产生的内源性产生的催化抗体。 还提供了用于调节基于核酸的治疗剂的功效的方法。

公开(公告)号：US5163865A

公开(公告)日：1992-11-17

申请号：US697089

申请日：1991-05-08

Applicant: Larry J. Smith

Inventor： Larry J. Smith

IPC: A22C25/18 ,  B26D1/08 ,  B26D1/60 ,  B26D7/06 ,  B26D7/08 ,  B26D7/18 ,  B26D7/30

CPC classification number: A22C17/0086 ,  A22C25/18 ,  B26D1/08 ,  B26D1/60 ,  B26D7/0625 ,  B26D7/086 ,  B26D7/18 ,  B26D7/30 ,  Y10S83/956 ,  Y10T83/543

Abstract: Disclosed is an apparatus and method for cutting or marking fish fillets and other foodstuffs of varying cross-section into portions of predetermined size by conveying each product through a volume scanning unit, computing correct cut locations, and then cutting or marking at each location using a guillotine-type knife mechanism. The guillotine-type knife mechanism moves in a longitudinal direction while cutting perpendicular to the conveyor surface. Dislocation of the fillet is eliminated by synchronizing knife speed with the conveyor, and by overcoming resistance of blade retraction with a retraction mechanism.

Abstract translation: 公开了一种用于通过将每个产品通过体积扫描单元输送，计算正确的切割位置，然后使用以下步骤在每个位置进行切割或标记来将鱼片和其它具有不同横截面的食品切割或标记为预定尺寸的部分的装置和方法： 闸刀式刀机构。 闸刀式刀机构在垂直于输送机表面进行切割的同时沿纵向移动。 通过将刀速与输送机同步，并通过采用缩回机构克服刀片退回的阻力来消除圆角脱位。

公开(公告)号：US5087617A

公开(公告)日：1992-02-11

申请号：US311096

申请日：1989-02-15

Applicant: Larry J. Smith

Inventor： Larry J. Smith

IPC: A61K31/70 ,  A61P35/00 ,  A61P35/02 ,  C07H21/00 ,  C12N15/09 ,  C12N15/113 ,  C12Q1/68

CPC classification number: A61K31/70 ,  C07H21/00 ,  C12N15/1135

Abstract: The present invention provides methods useful in autologous bone marrow transplantation and cancer therapy. According to one aspect of the invention, bone marrow cells from a patient having cancer are treated with selected antisense oligonucleotides in order to deplete the bone marrow of malignant cells prior to infusion back into the bone marrow donor. In a separate embodiment, selected antisense oligonucleotides are administered systemically for anticancer therapy.

Abstract translation: 本发明提供了在自体骨髓移植和癌症治疗中有用的方法。 根据本发明的一个方面，来自患有癌症的患者的骨髓细胞用选择的反义寡核苷酸处理，以在输回到骨髓供体之前消耗恶性细胞的骨髓。 在单独的实施方案中，选择的反义寡核苷酸全身用于抗癌治疗。