Transducing particles and methods for their production
    32.
    发明授权
    Transducing particles and methods for their production 失效
    用于生产粒子和方法

    公开(公告)号:US5187061A

    公开(公告)日:1993-02-16

    申请号:US609331

    申请日:1990-11-05

    摘要: Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria nmay be typed based on the pattern of reactivity observed. The transducing particles may be prepared by introducing a synthetic transposable element carrying the heterologous gene to a host carrying a prophage having the desired host range. After transposition, the host may be induced to a lytic cycle to release the transducing particles carrying the heterologous gene.

    摘要翻译: 可以通过将从样品获得的细菌培养物暴露于具有已知宿主范围的转导颗粒来检测生物样品中的活细菌。 这种转导颗粒携带能够以容易检测的方式改变细菌表型的异源基因。 例如,转导颗粒可以携带冰核基因,并且可以使用冰核化测定法检测表型的改变。 通过使用一组噬菌体,可以根据所观察到的反应模式来分类未知细菌。 可以通过将携带异源基因的合成转座元件引入携带具有所需宿主范围的原噬菌体的宿主来制备转导颗粒。 转座后,宿主可能被诱导到裂解循环以释放携带异源基因的转导颗粒。