利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

37 条记录 (耗时 0.026 秒)

    Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
    31.
    发明授权
    Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest 有权
    编码水蛭素和促胰岛素的核酸作为超滤肽并且用于平行改进感兴趣的一种或多种多肽的出口形式

    公开(公告)号：US07638618B2

    公开(公告)日：2009-12-29

    申请号：US10076631

    申请日：2002-02-19

    申请人： Paul Habermann

    发明人： Paul Habermann

    IPC分类号： C07H21/04 ,  C12P21/02 ,  C12N15/12 ,  C12N15/17

    CPC分类号： C07K14/815 ,  C07K14/62 ,  C07K2319/02 ,  C12N15/815 ,  C12P21/02

    摘要： A nucleic acid sequence including: Px-Sx-Bn-(ZR)-transport peptide-(Z1Z2)-protein(Y)-(Z1Z2)-protein(Ym)-T. The nucleic acid codes for a fusion protein including a peptide encoded by transport peptide linked via a peptide encoded by a first Z1Z2 to a protein encoded by protein(Y) which in turn is linked to T when m equals zero, or when m does not equal zero, is linked to a peptide encoded by a second Z1Z2which is linked to a chain comprising at least one and up to 5 proteins encoded by protein(Ym) which either correspond to the protein encoded by protein(Y) or can be different from the protein encoded by protein(Y). The peptide encoded by transport peptide improves the rate of secretion of the protein encoded by protein(Y) and the protein encoded by protein(Ym), when the protein encoded by protein(Ym) is present. Proteins thereof, host cells thereof, and processes thereof.

    摘要翻译： 包括：Px-Sx-Bn-（ZR） - 转运肽 - （Z1Z2） - 蛋白（Y） - （Z1Z2） - 蛋白（Ym）-T的核酸序列。 核酸编码融合蛋白，其包括由通过由第一Z1Z2编码的肽与由蛋白质（Y）编码的蛋白质连接的转运肽编码的肽，其在m等于零时连接到T，或者当m不为 与由第二个Z1Z2编码的肽连接，第二个Z1Z2连接到包含至少一个和多达五个由蛋白质（Ym）编码的蛋白质的链，所述蛋白质对应于由蛋白质（Y）编码的蛋白质，或可以不同于 由蛋白质（Y）编码的蛋白质。 由蛋白质（Ym）编码的蛋白质时，由转运肽编码的肽提高了由蛋白质（Y）和蛋白质（Ym）编码的蛋白质分泌的蛋白质的分泌速度。 其蛋白质，其宿主细胞及其过程。

    Signal sequences for preparing leu-hirudin by secretion by E. coli into the culture medium
    33.
    发明授权
    Signal sequences for preparing leu-hirudin by secretion by E. coli into the culture medium 有权
    用于通过大肠杆菌分泌到培养基中制备leu水蛭素的信号序列

    公开(公告)号：US07455987B1

    公开(公告)日：2008-11-25

    申请号：US09664326

    申请日：2000-09-18

    申请人： Paul Habermann ,  Rudolf Bender

    发明人： Paul Habermann ,  Rudolf Bender

    IPC分类号： C12P21/06

    CPC分类号： C12N15/625 ,  C07K14/815 ,  C07K2319/02 ,  C12N15/70

    摘要： The present invention relates to a efficient expression of a protein, particularly, hirudin; a protein precursor comprising a signal sequence and the sequence of aaX-hirudin, wherein aaX is a selected amino acid, and aaX is preferably leucine; to its preparation and use; and to processes for finding signal sequences for secretory expression of any desired protein in E coli; and to processes for the secretory expression of any desired protein in E coli.

    摘要翻译： 本发明涉及蛋白质，特别是水蛭素的有效表达; 其包含信号序列和aa×X-Hirudin的序列的蛋白质前体，其中aa是选定的氨基酸，aa是X 优选亮氨酸; 准备和使用; 以及找到用于在大肠杆菌中分泌表达任何所需蛋白质的信号序列的方法; 以及用于在大肠杆菌中分泌表达任何所需蛋白质的方法。

    Mini-proinsulin, its preparation and use
    34.
    发明授权
    Mini-proinsulin, its preparation and use 失效
    迷你胰岛素原，其制备和用途

    公开(公告)号：US06875589B1

    公开(公告)日：2005-04-05

    申请号：US08402394

    申请日：1995-03-10

    申请人： Michael Dörschug ,  Paul Habermann ,  Gerhard Seipke ,  Eugen Uhlmann

    发明人： Michael Dörschug ,  Paul Habermann ,  Gerhard Seipke ,  Eugen Uhlmann

    IPC分类号： A61K38/00 ,  C07K14/62 ,  C12N1/21 ,  C12N15/17 ,  A61K38/28 ,  C12N1/16

    CPC分类号： C07K14/62 ,  A61K38/00

    摘要： A mini-proinsulin, in which the amino acid Arg bridges the A and the B chain instead of the C chain, shows insulin activity and is suitable for the preparation of pharmaceuticals for the treatment of diabetes mellitus. It can furthermore be converted into an insulin derivative simply using trypsin, the B chain of which is lengthened by Arg. This can be converted into insulin using carboxypeptidase B. Advantageously, however, the mini-proinsulin can also be converted to insulin directly in a one-pot process.

    摘要翻译： 其中氨基酸Arg桥接A和B链而不是C链的微型胰岛素原显示胰岛素活性，并且适用于制备用于治疗糖尿病的药物。 胰蛋白酶可以简单地转化为胰岛素衍生物，其中B链被Arg延长。 这可以使用羧肽酶B转化成胰岛素。然而，有益的是，微型胰岛素原也可以在一锅法中直接转化为胰岛素。

    Fusion protein comprising an interleukin-2 fragment ballast portion
    35.
    发明授权
    Fusion protein comprising an interleukin-2 fragment ballast portion 失效
    包含白细胞介素-2片段压载物部分的融合蛋白

    公开(公告)号：US5496924A

    公开(公告)日：1996-03-05

    申请号：US194545

    申请日：1992-12-28

    申请人： Paul Habermann ,  Friedrich Wengenmayer

    发明人： Paul Habermann ,  Friedrich Wengenmayer

    IPC分类号： C07K14/535 ,  C07K14/55 ,  C07K14/62 ,  C07K14/815 ,  C07K14/47 ,  C07K19/00

    CPC分类号： C07K14/815 ,  C07K14/535 ,  C07K14/55 ,  C07K14/62 ,  C07K2319/00 ,  C07K2319/75

    摘要： A fusion protein including a ballast portion and a desired protein. The ballast portion forms either the C- or N-terminus of the fusion protein, and the ballast portion includes a part of the amino acid sequence of interleukin-2 (IL-2). According to certain preferred embodiments, the ballast portion includes approximately the first 100 amino acids of IL-2. According to other preferred embodiments, the ballast portion contains fewer than 100 amino acids of IL-2. According to further advantageous embodiments, a synthetic IL-2 gene is divided by unique cleavage sites into 6 segments, any number of these segments can be linked in arbitrary sequence. These embodiments permit specific instructions with which the solubility of the fusion protein can be altered, and thus the fusion proteins can readily be separated from soluble proteins intrinsic to a host. The DNA coding for the fusion proteins, as well as vectors and hosts are also provided.

    摘要翻译： 一种融合蛋白，包括压载物部分和所需的蛋白质。 镇流器部分形成融合蛋白的C-末端或N-末端，并且镇流部分包括白细胞介素-2（IL-2）的氨基酸序列的一部分。 根据某些优选实施方案，镇流器部分包括大约IL-2的前100个氨基酸。 根据其它优选实施方案，镇流器部分含有少于100个IL-2的氨基酸。 根据进一步有利的实施方案，将合成的IL-2基因由唯一的切割位点分成6个区段，任何数目的这些区段可以以任意顺序连接。 这些实施方案允许可以改变融合蛋白的溶解度的特异性指导，因此融合蛋白可容易地从主体固有的可溶性蛋白质中分离出来。 还提供了编码融合蛋白的DNA，以及载体和宿主。

    Method of preparing fusion proteins
    37.
    发明授权
    Method of preparing fusion proteins 失效
    融合蛋白的制备方法

    公开(公告)号：US5358857A

    公开(公告)日：1994-10-25

    申请号：US73508

    申请日：1993-06-09

    申请人： Siegfried Stengelin ,  Paul Habermann ,  Eugen Uhlmann ,  Brian Seed

    发明人： Siegfried Stengelin ,  Paul Habermann ,  Eugen Uhlmann ,  Brian Seed

    IPC分类号： C07K14/62 ,  C12N15/12 ,  C12N15/62

    CPC分类号： C07K14/62 ,  C12N15/62 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/35 ,  C07K2319/75

    摘要： Fusion proteins are obtained in high yields if a mixed oligonucleotide is constructed which codes for the ballast constituent of the fusion protein. The oligonucleotide mixture is introduced in a vector in such a manner that it is functionally linked to a regulatory region and to the structural gene for the desired protein. Appropriate host cells are transformed with the plasmid population obtained in this manner and the clones producing a high yield of coded fusion protein are selected.

    摘要翻译： 如果构建编码融合蛋白的镇流成分的混合寡核苷酸，则以高产率获得融合蛋白。 将寡核苷酸混合物以这样的方式引入载体中，使得其功能上连接到调节区域和所需蛋白质的结构基因。 用以这种方式获得的质粒群转化合适的宿主细胞，并选择产生高产量的编码融合蛋白的克隆。