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    Signal sequences for preparing leu-hirudin by secretion by E. coli into the culture medium
    1.
    发明授权
    Signal sequences for preparing leu-hirudin by secretion by E. coli into the culture medium 有权
    用于通过大肠杆菌分泌到培养基中制备leu水蛭素的信号序列

    公开(公告)号：US07455987B1

    公开(公告)日：2008-11-25

    申请号：US09664326

    申请日：2000-09-18

    申请人： Paul Habermann ,  Rudolf Bender

    发明人： Paul Habermann ,  Rudolf Bender

    IPC分类号： C12P21/06

    CPC分类号： C12N15/625 ,  C07K14/815 ,  C07K2319/02 ,  C12N15/70

    摘要： The present invention relates to a efficient expression of a protein, particularly, hirudin; a protein precursor comprising a signal sequence and the sequence of aaX-hirudin, wherein aaX is a selected amino acid, and aaX is preferably leucine; to its preparation and use; and to processes for finding signal sequences for secretory expression of any desired protein in E coli; and to processes for the secretory expression of any desired protein in E coli.

    摘要翻译： 本发明涉及蛋白质，特别是水蛭素的有效表达; 其包含信号序列和aa×X-Hirudin的序列的蛋白质前体，其中aa是选定的氨基酸，aa是X 优选亮氨酸; 准备和使用; 以及找到用于在大肠杆菌中分泌表达任何所需蛋白质的信号序列的方法; 以及用于在大肠杆菌中分泌表达任何所需蛋白质的方法。

    Method of an apparatus for lasting sides of shoes on lasts, comprising a side tack insertion device
    2.
    发明授权
    Method of an apparatus for lasting sides of shoes on lasts, comprising a side tack insertion device 失效
    一种用于鞋的持久侧的装置的方法，包括侧钉插入装置

    公开(公告)号：US4630325A

    公开(公告)日：1986-12-23

    申请号：US842759

    申请日：1986-03-21

    申请人： Rudolf Bender ,  Hans-Gerd Dietrich ,  Gerhard Giebel

    发明人： Rudolf Bender ,  Hans-Gerd Dietrich ,  Gerhard Giebel

    IPC分类号： A43D21/00 ,  A43D75/00 ,  A43D21/12 ,  A43D23/02

    CPC分类号： A43D21/003 ,  A43D75/00

    摘要： Method of and apparatus for side lasting shoes on lasts comprising a side tack insertion device, wherein the latter is moved along the lasting margin in an operating path following the course of the shank region. A friction closure element (tensioning strap, roller, smoothing runner) is moved stepwise in a longitudinal direction of the shoe and with each step is pressed periodically against the upper in a strip-like region associated with each tack insertion, whereby the upper is stretched in a direction towards the lasting margin.

    摘要翻译： 用于在持续时间上的侧面持久鞋的方法和装置，包括侧钉插入装置，其中后者沿着沿着柄部区域的过程的操作路径中的持续边缘移动。 摩擦闭合元件（张紧带，滚子，平滑流道）在鞋的纵向方向上逐步移动，并且每个步骤在与每个钉插入相关联的条状区域中周期性地压靠在鞋面上，由此上部被拉伸 朝向持久的边缘的方向。

    Method for measuring the wall thickness of a workpiece by ultra-sound
    5.
    发明授权
    Method for measuring the wall thickness of a workpiece by ultra-sound 失效
    通过超声波测量工件的壁厚的方法

    公开(公告)号：US4838086A

    公开(公告)日：1989-06-13

    申请号：US138730

    申请日：1987-12-29

    申请人： Rudolf Bender ,  Jorg Quittkat

    发明人： Rudolf Bender ,  Jorg Quittkat

    IPC分类号： G01B17/02

    CPC分类号： G01B17/02 ,  Y10S73/901

    摘要： A method and an apparatus for measuring the wall thickness of a workpiece by ultra-sound is disclosed. For measuring the wall thickness, it is necessary to determine the time interval between a surface echo occurring during irradiation by ultra-sound and a rear wall echo. According to the present invention, a saw tooth generator generates a saw tooth signal including a sequence of saw teeth when the surface echo occurs. Each of the saw teeth causes a counting pulse to be generated. Upon occurrence of the rear wall echo, the instantaneous amplitude value of the saw tooth signal is measured and the number of counting pulses is recorded by a counter. Each counting pulse defines a constant unit of wall thickness. The amplitude value provides a fraction of such wall thickness unit.

    摘要翻译： 公开了一种通过超声测量工件的壁厚的方法和装置。 为了测量壁厚，有必要确定在超声辐照期间发生的表面回波与后壁回波之间的时间间隔。 根据本发明，当发生表面回波时，锯齿发生器产生包括锯齿序列的锯齿信号。 每个锯齿导致产生计数脉冲。 在出现后壁回波时，测量锯齿信号的瞬时振幅值，计数器记录计数脉冲数。 每个计数脉冲定义了一个恒定的壁厚单位。 振幅值提供了这种壁厚单位的一小部分。