公开(公告)号：US20130247234A1

公开(公告)日：2013-09-19

申请号：US13832247

申请日：2013-03-15

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： John MCWHIRTER ,  Lynn MACDONALD ,  Andrew J. MURPHY

IPC: A01K67/027

CPC classification number: C07K16/28 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/00 ,  C07K16/468 ,  C07K2317/515 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12N15/8509 ,  C12N2800/204

Abstract: A genetically modified non-human animal is provided, wherein the non-human animal expresses an antibody repertoire capable of pH dependent binding to antigens upon immunization. A genetically modified non-human animal is provided that expresses a single light chain variable domain derived from a single rearranged light chain variable region gene in the germline of the non-human animal, wherein the single rearranged light chain variable region gene comprises a substitution of at least one non-histidine encoding codon with a histidine encoding codon. Methods of making non-human animals that express antibodies comprising a histidine-containing universal light chain are provided.

Abstract translation: 提供了遗传修饰的非人动物，其中所述非人动物在免疫时表达能够与抗原结合的抗体。 提供了一种遗传修饰的非人动物，其表达来自非人动物种系中的单个重排轻链可变区基因的单个轻链可变结构域，其中单重排列的轻链可变区基因包含 编码密码子组氨酸的至少一个非组氨酸编码密码子。 提供了表达含有组氨酸通用轻链的抗体的非人动物的方法。

公开(公告)号：US20130185820A1

公开(公告)日：2013-07-18

申请号：US13793935

申请日：2013-03-11

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： Lynn MACDONALD ,  Andrew J. MURPHY ,  Naxin TU ,  Cagan GURER ,  Vera VORONINA ,  Sean STEVENS

IPC: A01K67/027

CPC classification number: A01K67/0278 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  A61K39/00 ,  C07H21/04 ,  C07K14/70539 ,  C12N15/8509 ,  C12N2830/001

Abstract: The invention provides genetically modified non-human animals that express a humanized MHC II protein (humanized MHC II α and β polypeptides), as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified animals and methods of making the same. Methods of using the genetically modified animals to study various aspects of human immune system are provided.

Abstract translation: 本发明提供表达人源化MHC II蛋白（人源化MHCIIα和β多肽）以及包含其的组织，细胞和组织的遗传修饰的非人动物。 还提供了用于制备所述转基因动物的构建体及其制备方法。 提供了使用遗传修饰动物研究人体免疫系统各个方面的方法。

公开(公告)号：US20130111617A1

公开(公告)日：2013-05-02

申请号：US13661159

申请日：2012-10-26

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： Lynn MACDONALD ,  Andrew J. MURPHY ,  Cagan GURER ,  John MCWHIRTER ,  Vera VORONINA ,  Faith HARRIS ,  Sean STEVENS

IPC: A01K67/027 ,  A01K67/02 ,  C12N15/06

CPC classification number: A01K67/0278 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/03 ,  C07K14/70539

Abstract: The invention provides genetically modified non-human animals that express chimeric human/non-human MHC I polypeptide and/or human or humanized β2 microglobulin polypeptide, as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified animals and methods of making the same. Methods of using the genetically modified animals to study various aspects of human immune system are provided.

Abstract translation: 本发明提供表达嵌合人/非人MHC I多肽和/或人或人源化β2微球蛋白多肽以及包含其的胚胎，细胞和组织的遗传修饰的非人动物。 还提供了用于制备所述转基因动物的构建体及其制备方法。 提供了使用遗传修饰动物研究人体免疫系统各个方面的方法。