利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

38 条记录 (耗时 0.032 秒)

    Cells and animals produced by gene silencing
    31.
    发明申请
    Cells and animals produced by gene silencing 有权
    通过基因沉默产生的细胞和动物

    公开(公告)号：US20050102710A1

    公开(公告)日：2005-05-12

    申请号：US11013531

    申请日：2004-12-17

    申请人： David Baulcombe ,  Andrew Hamilton

    发明人： David Baulcombe ,  Andrew Hamilton

    IPC分类号： C12N15/82 ,  A01K67/033 ,  A01H1/00 ,  C12Q1/68

    CPC分类号： C12N15/113 ,  A01N57/16 ,  C12N15/8218

    摘要： Provided are methods of screening for the occurrence of gene silencing (e.g. post transcriptional gene silencing) in an organism (e.g. a plant or animal), which method comprises the steps of: (i) obtaining a sample of material from said organism, (ii) producing a nucleic acid extract from said sample, (iii) analysing said extract such as to determine the presence or absence of short RNA molecules which are approximately 25 nucleotides in length (SRMs) in said nucleic extract, (iv) correlating the presence of said SRMs in the extract with the occurrence of gene silencing in said organism. The SRMs are preferably short anti-sense RNA molecules (SARMs). Also provided are associated methods for detecting the silencing of a target gene in an organism. Processes for isolating one or more RNA molecules, such as SARMs, which may be advantageously employed in the method, may include a purification step selected from (i) filtration; (ii) differential precipitation (iii) ion exchange chromatography, followed-by separation the purified RNA molecules according to size by electrophoresis through 15% polyacrylamide gel containing 7M urea as a denaturant and TBE (0.5×) as a buffer, and blotting by electrophoresis. Also provided are processes for isolating silencing agents comprising SRMs; methods of selecting target regions of target genes for directed silencing; and methods of silencing target genes in organisms based on these. Silencing in a first organism may be achieved by generating in a second organism, SRMs which are introduced into the first organism such as to silence a target gene therein. Also provided are DNA constructs, host cells, plants and non-human mammals which comprise target genes which have been silenced in accordance with the methods herein.

    摘要翻译： 提供了筛选生物体（例如植物或动物）中基因沉默（例如转录后基因沉默）的发生的方法，该方法包括以下步骤：（i）从所述生物体获得材料样品，（ii 产生来自所述样品的核酸提取物，（iii）分析所述提取物，以确定在所述核酸提取物中长度为约25个核苷酸（SRM）的短RNA分子的存在或不存在，（iv） 所述提取物中所述SRM在所述生物体中发生基因沉默。 SRM优选为短反义RNA分子（SARM）。 还提供了用于检测生物体中靶基因沉默的相关方法。 用于分离一种或多种RNA分子（例如SARM）的方法，其可以有利地用于该方法中，可以包括纯化步骤，其选自（i）过滤; （ii）差示沉淀（iii）离子交换色谱法，随后通过以含有7M尿素作为变性剂和TBE（0.5x）作为缓冲液的15％聚丙烯酰胺凝胶电泳，根据大小分离纯化的RNA分子，并通过电泳印迹 。 还提供了用于分离包含SRM的沉默剂的方法; 选择目标基因靶向定向沉默的方法; 以及基于这些沉默生物体中靶基因的方法。 在第一生物体中的沉默可以通过在第二生物体中产生被引入第一生物体的SRM来实现，以使其中的靶基因沉默。 还提供了包括根据本文方法已经沉默的靶基因的DNA构建体，宿主细胞，植物和非人哺乳动物。

    Method and system for automated teller machine online/offline operation
    33.
    发明申请
    Method and system for automated teller machine online/offline operation 审中-公开
    自动柜员机在线/离线操作的方法和系统

    公开(公告)号：US20070162389A1

    公开(公告)日：2007-07-12

    申请号：US11328324

    申请日：2006-01-09

    申请人： Andrew Hamilton ,  Stephen MacPhail ,  Wolf Rossmann

    发明人： Andrew Hamilton ,  Stephen MacPhail ,  Wolf Rossmann

    IPC分类号： G06Q40/00

    CPC分类号： G07F19/211 ,  G06Q20/1085 ,  G07F19/20 ,  G07F19/206

    摘要： Methods, systems, and software are disclosed for configuring an ATM. The ATM receives an online/offline configuration message that specifies one or more conditions when the ATM is to go online. The ATM goes offline and performing zero or more offline transactions. The ATM goes online based at least in part on at least a portion of the online/offline configuration message. The online/offline configuration message is in an XML-based format.

    摘要翻译： 公开了用于配置ATM的方法，系统和软件。 ATM接收到一个在线/离线配置消息，指定ATM上线时的一个或多个条件。 ATM脱机并执行零个或多个脱机事务。 ATM至少部分地基于至少一部分在线/离线配置消息在线。 在线/离线配置消息是基于XML的格式。

    Gene silencing
    34.
    发明申请
    Gene silencing 审中-公开
    基因沉默

    公开(公告)号：US20060168669A1

    公开(公告)日：2006-07-27

    申请号：US11390519

    申请日：2006-03-27

    申请人： David Baulcombe ,  Andrew Hamilton

    发明人： David Baulcombe ,  Andrew Hamilton

    IPC分类号： A01K67/00 ,  A01H1/00

    CPC分类号： C12N15/113 ,  A01N57/16 ,  C12N15/8218

    摘要： Provided are methods of screening for the occurrence of gene silencing (e.g. post transcriptional gene silencing) in an organism (e.g. a plant or animal), which method comprises the steps of: (i) obtaining a sample of material from said organism, (ii) producing a nucleic acid extract from said sample, (iii) analysing said extract such as to determine the presence or absence of short RNA molecules which are approximately 25 nucleotides in length (SRMs) in said nucleic extract, (iv) correlating the presence of said SRMs in the extract with the occurrence of gene silencing in said organism. The SRMs are preferably short anti-sense RNA molecules (SARMs). Also provided are associated methods for detecting the silencing of a target gene in an organism. Processes for isolating one or more RNA molecules, such as SARMs, which may be advantageously employed in the method, may include a purification step selected from (i) filtration; (ii) differential precipitation (iii) ion exchange chromatography, followed by separation the purified RNA molecules according to size by electrophoresis through 15% polyacrylamide gel containing 7M urea as a denaturant and TBE (0.5×) as a buffer, and blotting by electrophoresis. Also provided are processes for isolating silencing agents comprising SRMs; methods of selecting target regions of target genes for directed silencing; and methods of silencing target genes in organisms based on these. Silencing in a first organism may be achieved by generating in a second organism, SRMs which are introduced into the first organism such as to silence a target gene therein. Also provided are DNA constructs, host cells, plants and non-human mammals which comprise target genes which have been silenced in accordance with the methods herein.

    摘要翻译： 提供了筛选生物体（例如植物或动物）中基因沉默（例如转录后基因沉默）的发生的方法，该方法包括以下步骤：（i）从所述生物体获得材料样品，（ii 产生来自所述样品的核酸提取物，（iii）分析所述提取物，以确定在所述核酸提取物中长度为约25个核苷酸（SRM）的短RNA分子的存在或不存在，（iv） 所述提取物中所述SRM在所述生物体中发生基因沉默。 SRM优选为短反义RNA分子（SARM）。 还提供了用于检测生物体中靶基因沉默的相关方法。 用于分离一种或多种RNA分子（例如SARM）的方法，其可以有利地用于该方法中，可以包括纯化步骤，其选自（i）过滤; （ii）差示沉淀（iii）离子交换层析，然后根据大小通过电泳，通过含有7M尿素作为变性剂和TBE（0.5x）作为缓冲液的15％聚丙烯酰胺凝胶分离纯化的RNA分子，并通过电泳印迹。 还提供了用于分离包含SRM的沉默剂的方法; 选择目标基因靶向定向沉默的方法; 以及基于这些沉默生物体中靶基因的方法。 在第一生物体中的沉默可以通过在第二生物体中产生被引入第一生物体的SRM来实现，以使其中的靶基因沉默。 还提供了包括根据本文方法已经沉默的靶基因的DNA构建体，宿主细胞，植物和非人哺乳动物。

    RNA molecules and vectors for gene silencing
    36.
    发明申请
    RNA molecules and vectors for gene silencing 有权

    公开(公告)号：US20050100950A1

    公开(公告)日：2005-05-12

    申请号：US11013316

    申请日：2004-12-17

    申请人： David Baulcombe ,  Andrew Hamilton

    发明人： David Baulcombe ,  Andrew Hamilton

    IPC分类号： C12N15/82 ,  C12Q1/68

    CPC分类号： C12N15/113 ,  A01N57/16 ,  C12N15/8218

    摘要： Provided are methods of screening for the occurrence of gene silencing (e.g. post transcriptional gene silencing) in an organism (e.g. a plant or animal), which method comprises the steps of; (i) obtaining a sample of material from said organism, (ii) producing a nucleic acid extract from said sample, (iii) analysing said extract such as to determine the presence or absence of short RNA molecules which are approximately 25 nucleotides in length (SRMs) in said nucleic extract, (iv) correlating the presence of said SRMs in the extract with the occurrence of gene silencing in said organism. The SRMs are preferably short anti-sense RNA molecules (SARMs). Also provided are associated methods for detecting the silencing of a target gene in an organism. Processes for isolating one or more RNA molecules, such as SARMs, which may be advantageously employed in the method, may include a purification step selected from (i) filtration; (ii) differential precipitation (iii) ion exchange chromatography, followed by separation the purified RNA molecules according to size by electrophoresis through 15% polyacrylamide gel containing 7M urea as a denaturant and TBE (0.5×) as a buffer, and blotting by electrophoresis. Also provided are processes for isolating silencing agents comprising SRMs; methods of selecting target regions of target genes for directed silencing; and methods of silencing target genes in organisms based on these. Silencing in a first organism may be achieved by generating in a second organism, SRMs which are introduced into the first organism such as to silence a target gene therein. Also provided are DNA constructs, host cells, plants and non-human mammals which comprise target genes which have been silenced in accordance with the methods herein.

    Applicator having an extension
    38.
    发明授权
    Applicator having an extension 失效
    具有延长的施放器

    公开(公告)号：US6101657A

    公开(公告)日：2000-08-15

    申请号：US511192

    申请日：1995-08-04

    申请人： Andrew Hamilton

    发明人： Andrew Hamilton

    IPC分类号： A46B17/02 ,  B25G1/04 ,  B25G1/06 ,  A46B7/02 ,  B25G3/30

    CPC分类号： A46B17/02 ,  B25G1/04 ,  B25G1/06 ,  Y10T16/455

    摘要： A paintbrush connectable to an extension is provided. The paintbrush has a threaded bore for threaded engagement with an extension pole. At least one handle having a sleeve portion can be slid over the extension pole and secured thereto to facilitate more accurate control of the paintbrush. The handle is infinitely adjustable along the length of the extension pole.

    摘要翻译： 提供可连接到扩展的画笔。 画笔具有用于与延伸杆螺纹接合的螺纹孔。 具有套筒部分的至少一个手柄可以在延伸杆上滑动并固定到其上，以便更准确地控制画笔。 手柄沿着延伸杆的长度无级调节。