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31.发明授权
公开(公告)号：US5096814A
公开(公告)日：1992-03-17
申请号：US456422
申请日：1989-12-29
CPC分类号： C12N11/14 , C02F3/10 , C12N1/00 , Y02E50/343 , Y02W10/15
摘要： For the immobilization of micro-organisms and animal cells, in particular for anaerobic processes, such as the purification of waste water or for the biotechnological production of nutrition-essential or pharmacological substances, porous, sintered bodies are employed (inorganic carrier bodies). In particular, sintered glass in the form of Raschig rings with a double-pore structure, are employed. They have porosity-determining through-going macropores that permit a free exchange of fluid and gas from the interior of the carrier to the surroundings, and open micropores within the macropore walls, the diameter of the micropores being of the same order of magnitude as the size of the micro-organisms or cells. These carrier bodies typically have an open pore volume of 35% to 85%, 20% to 80% being accounted for by the macropores having a diameter of 20 to 500 .mu.m, and 5%-50% by micropores having a diameter of 1-10 .mu.m. These bodies are obtained by sintering a powder mixture comprising fine-grain material and a coarse-grain substance melting at a higher-than-sintering temperature and separable from the sintered product by allowing the latter to cool and separating (dissolving) out the soluble component.
摘要翻译： 对于微生物和动物细胞的固定，特别是用于厌氧过程，例如净化废水或生物技术生产营养必需或药物物质，使用多孔烧结体（无机载体）。 特别地，采用具有双孔结构的Raschig环形式的烧结玻璃。 它们具有孔隙度确定的穿孔大孔，其允许流体和气体从载体的内部自由交换到周围环境，并且在大孔内部开放微孔，微孔的直径与 微生物或细胞的大小。 这些载体通常具有35％至85％的开孔体积，通过直径为20至500μm的大孔占20％至80％，直径为1的微孔占5％-50％ -10亩。 这些体是通过烧结包含细粒材料和粗晶粒物质的粉末混合物而获得的，所述粉末混合物以高于烧结温度熔化并与烧结产物分离，通过使其冷却并分离（溶解）可溶组分 。
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公开(公告)号：US5057415A
公开(公告)日：1991-10-15
申请号：US251176
申请日：1988-09-30
CPC分类号： C07K1/20 , C07K1/02 , C07K5/00 , Y02P20/55 , Y10S435/803 , Y10S435/813
摘要： Peptide preparation is carried out by a continuous process by supplying a serine protease or peptidase enzyme retained in a reaction vessel with an alkyl ester of an N-protected amino acid or oligopeptide and a recycle stream containing an amino acid or oligopeptide to form an N-protected chain extended peptide, separating the N-protected chain extended peptide by adsorption on a hydrophobic absorbent, and eluting and recovering the adsorbed N-protected chain extended peptide. Adsorption is carried out without adjusting the pH from that in the reaction vessel, and adsorber effluent is recycled to the recycle stream. The protease or peptidase enzyme may be immobilized, and there is substantial exclusion of organic solubilizers in the reaction vessel. A preferred N-protecting group is an N-phenacyl group. The N-protecting group can be separated from the N-protected chain extended peptide with a deprotecting enzyme to recover the chain extended peptide. Apparatus is used that provides for continuous operation of the process.
摘要翻译： 通过将保留在反应容器中的丝氨酸蛋白酶或肽酶与N-保护的氨基酸或寡肽的烷基酯和含有氨基酸或寡肽的再循环物流相连，形成N- 受保护的链延长肽，通过在疏水性吸收剂上的吸附分离N-保护的链延长肽，并洗脱和回收吸附的N-保护的链延长肽。 在不将pH调节到反应容器内的pH的条件下进行吸附，并将吸附剂流出物再循环到循环流中。 可以固定蛋白酶或肽酶，并且在反应容器中基本排除有机增溶剂。 优选的N-保护基是N-苯甲酰甲酰基。 可以用去保护酶将N-保护基与N-保护的链延长肽分离，以回收链延长肽。 使用提供该方法的连续操作的装置。
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公开(公告)号：US4927751A
公开(公告)日：1990-05-22
申请号：US882349
申请日：1986-07-07
申请人： Klaus Memmert , Christian Wandrey
发明人： Klaus Memmert , Christian Wandrey
CPC分类号： C12Y302/01032 , C12N9/00 , C12N9/2417 , C12N9/248 , C12N9/54 , C12Y302/01008 , Y10S435/813 , Y10S435/818
摘要： Exoenzymes, such as proteases, xylanases and amylases, are obtained continuously by cultivation of exoenzyme-producing microorganisms in one step in a fermenter which is operated with continuous flow and in which a deficiency state corresponding to maximal enzyme productivity is effected. Optical density of the culture (as a measure of the biomass density) and exoenzyme concentration in culture can be monitored to control the timing and extent of the deficiency state. It is particularly advantageous to impose an oxygen limitation and to maintain the deficiency state continuously by exerting an effect on the oxygen input.
摘要翻译： 通过在连续流动运行的发酵罐中一步培养产生副产菌的微生物，并且其中产生与最大酶生产力相应的缺陷状态，连续获得外切酶，例如蛋白酶，木聚糖酶和淀粉酶。 可以监测培养物的光密度（作为生物量密度的量度）和培养物中的外切酶浓度，以控制缺陷状态的时间和程度。 特别有利的是施加氧气限制并通过对氧气输入施加影响来连续地维持缺陷状态。
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