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    Continuous production of an instant corn flour for snack and tortilla, using a neutral enzymatic precooking
    4.
    发明授权
    Continuous production of an instant corn flour for snack and tortilla, using a neutral enzymatic precooking 有权
    连续生产即食玉米粉的小吃和玉米粉，使用中性酶预煮

    公开(公告)号：US07459174B2

    公开(公告)日：2008-12-02

    申请号：US10973381

    申请日：2004-10-27

    申请人： Felipe A. Rubio ,  Manuel J. Rubio ,  Roberto Contreras ,  J. Fernando Ramirez ,  Rodrigo Lobeira Massu

    发明人： Felipe A. Rubio ,  Manuel J. Rubio ,  Roberto Contreras ,  J. Fernando Ramirez ,  Rodrigo Lobeira Massu

    IPC分类号： A23F3/16

    CPC分类号： A21D8/042 ,  A21D13/42 ,  A23K10/37 ,  A23L7/107 ,  A23L7/115 ,  A23L7/198 ,  B02B1/08 ,  B02B5/02 ,  C12Y302/01001 ,  C12Y302/01032 ,  Y02P60/877

    摘要： Precooked and partially-debranned corn flour is continuously produced by an enzymatic precooking using a commercial blend of xylanase, endoamylase and endoprotease as a processing aid. The low-temperature and neutral-pH precooking with an endoenzyme solution effected a partial bran hydrolysis while avoiding excessive pregelatinization, reduced washing and corn solid loss in wastewater. Moisture content is then stabilized, followed by milling and drying at a high-temperature and short-time to produce a controlled gelatinization and denaturation in the ground kernel, cooling and further drying the dried-ground particle. A fine particle size or flour is separated and recovered from the coarser particle which is also segregated to isolate a partially hydrolysed bran fraction for integral flour or animal feed diet, remilling and sieving the coarser particle to produce an instant corn flour for snack, and admixing the fine particle with lime to obtain a masa flour for tortilla and other corn foods.

    摘要翻译： 通过使用木聚糖酶，内切酶和内切蛋白酶的商业混合物作为加工助剂，通过酶预预煮连续生产预煮和部分脱氨基的玉米粉。 用酶溶液预处理的低温和中性pH预处理可以进行局部的麸皮水解，同时避免过度的预胶化，减少洗涤和废水中的玉米固体损失。 然后使水分含量稳定，然后在高温和短时间内研磨和干燥，以在研磨的核中产生受控的糊化和变性，冷却并干燥干燥的颗粒。 从较粗颗粒分离并回收细粒度或面粉，其也被分离，以分离用于整体面粉或动物饲料饮食的部分水解的麸皮部分，重新​​加工和筛分较粗颗粒以产生用于小吃的速溶玉米粉，并混合 用石灰精细的颗粒，得到玉米粉和其他玉米食品的泥沙粉。

    Mutated Xylanase Gene with High Reaction Activity and Site-Specific Mutagenesis Method Thereof
    5.
    发明申请
    Mutated Xylanase Gene with High Reaction Activity and Site-Specific Mutagenesis Method Thereof 有权
    具有高反应活性和位点特异性诱变方法的突变木聚糖酶基因

    公开(公告)号：US20080254539A1

    公开(公告)日：2008-10-16

    申请号：US11851593

    申请日：2007-09-07

    申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

    发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

    IPC分类号： C12N15/01 ,  C07H21/00

    CPC分类号： C12N9/2485 ,  C12Y302/01032

    摘要： A mutated xylanase gene with high reaction activity includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

    摘要翻译： 具有高反应活性的突变的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成突变的木聚糖酶基因。

    Bacterial protein with xylanase activity
    7.
    发明授权
    Bacterial protein with xylanase activity 失效
    具有木聚糖酶活性的细菌蛋白

    公开(公告)号：US06200797B1

    公开(公告)日：2001-03-13

    申请号：US08817946

    申请日：1997-10-10

    申请人： Robert William Dunlop ,  Bin Wang ,  Diane Ball ,  Alexander Buhisan Roullo ,  Cedric John Falk

    发明人： Robert William Dunlop ,  Bin Wang ,  Diane Ball ,  Alexander Buhisan Roullo ,  Cedric John Falk

    IPC分类号： C12N120

    CPC分类号： C12Y302/01032 ,  A23K20/189 ,  C12N9/248 ,  C12R1/125 ,  C12Y302/01008 ,  D21C5/005 ,  Y10S435/832 ,  Y10S435/839

    摘要： The invention relates to specific bacterium and proteins with xylanase activity derived from the bacteria, in particular to xylanases which are free of any significant cellulase activity and which are active at high temperature and at neutral to alkaline pH. Xylanases having these characteristics are particularly useful in the bleaching of wood pulps, such as kraft pulps. The preferred bacterium designated B230 was isolated from white-rotted kerri wood in Western Australia; a sample of which has been deposited under the provision of the Budapest Treaty in the Australian Government Analytical Laboratories under the accession number N94/41262. This preferred bacterium is a gram positive, obligatively aerobic, rod-shaped with a centrally-located spore and has the taxomonic characteristics of Bacillus subtilis (by VITEK method).

    摘要翻译： 本发明涉及具有来源于细菌的木聚糖酶活性的具体细菌和蛋白质，特别是不含任何​​明显的纤维素酶活性并在高温和中性至碱性pH下具有活性的木聚糖酶。 具有这些特征的木聚糖酶特别可用于木浆的漂白，例如硫酸盐纸浆。 从澳大利亚西澳的白萝卜切片中分离出名为B230的优选细菌; 其样本根据布达佩斯条约的规定在澳大利亚政府分析实验室存放，登记号为N94 / 41262。 这种优选的细菌是革兰氏阳性的，必需的需氧的，具有中心位置孢子的棒状，并具有枯草芽孢杆菌的分子特征（通过VITEK方法）。