Method of and system for analyzing a sample containing a plurality of fluorescent substances
    42.
    发明授权
    Method of and system for analyzing a sample containing a plurality of fluorescent substances 失效
    用于分析含有多种荧光物质的样品的方法和系统

    公开(公告)号:US06864975B2

    公开(公告)日:2005-03-08

    申请号:US10310032

    申请日:2002-12-05

    摘要: The present invention proposes a method of and system for analyzing samples (particularly, bio-samples), wherein the continuous spectrum white light pulse generated from an ultra-short optical pulse is used in a simple manner. By a system according to the invention, continuous spectrum white light pulses generated by converging ultra-short optical pulses on a point in a transparent substance 23 are dispersed into a spectrum of light by a dispersing element 25, and a plurality of objective wavelength components are separately selected by small reflection mirrors 37, masked mirrors or the like. The selected objective wavelength components are combined by the dispersing element 25 into a composite optical pulse containing the objective wavelength components. The composite optical pulse is irradiated onto the bio-sample 28 or other samples, whereby only the desired fluorescent substances are excited by the objective wavelength components corresponding to them. The selected objective wavelength components may preferably have different light path lengths. By this construction, the component optical pulses corresponding to the objective wavelengths in the composite optical pulse are separated from each other, so that the noise due to the interference of different wavelength components is prevented.

    摘要翻译: 本发明提出了一种用于分析样品(特别是生物样品)的方法和系统,其中以简单的方式使用从超短光脉冲产生的连续光谱白光脉冲。 通过根据本发明的系统,通过在透明物质23中的点聚焦超短光脉冲而产生的连续光谱白光脉冲通过分散元件25分散到光谱中,并且多个目标波长分量是 通过小反射镜37分别选择掩模镜等。 所选择的目标波长分量由分散元件25组合成包含目标波长分量的复合光脉冲。 将复合光脉冲照射到生物样品28或其他样品上,由此只有所需的荧光物质被与它们相对应的目标波长成分激发。 所选择的目标波长分量可以优选地具有不同的光路长度。 通过这种结构,与复合光脉冲中的目标波长相对应的分量光脉冲彼此分离,从而防止了由于不同波长分量的干扰引起的噪声。