公开(公告)号：US06383762B1

公开(公告)日：2002-05-07

申请号：US09145864

申请日：1998-09-02

申请人： Hung-Teh Kao ,  Paul R. Hartig ,  Theresa Branchek

发明人： Hung-Teh Kao ,  Paul R. Hartig ,  Theresa Branchek

IPC分类号： G01N33566

CPC分类号： C07K14/70571

摘要： The present invention provides a method of obtaining a composition which comprises determining whether a chemical compound binds to a human 5-HT2 receptor expressed on the surface of a mammalian cell transfected with a vector adapted for expressing the receptor in the cell, and if the compound binds to the receptor, admixing the compound with a carrier. The present invention further provides a method of obtaining a composition which comprises determining whether a chemical compound binds to and activates or binds to and inhibits activation of a human 5-HT2 receptor expressed on the surface of a mammalian cell, wherein the human 5-HT2 receptor is expressed on the surface of a mammalian cell transfected with a vector adapted for expressing the receptor in the cell, and if the compound binds to and activates or binds to and inhibits activation of the receptor, admixing the compound with a carrier.

摘要翻译： 本发明提供一种获得组合物的方法，其包括确定化学化合物是否结合在用适于在细胞中表达受体的载体转染的哺乳动物细胞表面上表达的人5-HT2受体，如果化合物 与受体结合，将化合物与载体混合。 本发明还提供一种获得组合物的方法，其包括确定化学化合物是否结合并激活或结合并抑制在哺乳动物细胞表面上表达的人5-HT 2受体的活化，其中人5-HT2 受体在用适于在细胞中表达受体的载体转染的哺乳动物细胞的表面上表达，并且如果化合物结合并激活或结合并抑制受体活化，则将化合物与载体混合。

公开(公告)号：US5885785A

公开(公告)日：1999-03-23

申请号：US613044

申请日：1996-03-08

申请人： Hung-Teh Kao ,  Paul R. Hartig ,  Theresa Branchek

发明人： Hung-Teh Kao ,  Paul R. Hartig ,  Theresa Branchek

IPC分类号： C07K14/705 ,  G01N33/566 ,  C12N5/16 ,  C12N15/12

CPC分类号： C07K14/70571

摘要： The present invention provides an isolated nucleic acid molecule encoding a 5-HT.sub.2 receptor, and an isolated protein which is a human 5-HT.sub.2 receptor. The invention also provides vectors comprising DNA molecules encoding a human 5-HT.sub.2 receptor, and vectors adapted for expression of the 5-HT.sub.2 receptor in bacterial, yeast, or mammalian cells. In addition, the invention provides a DNA probe useful for detecting nucleic acid encoding the 5-HT.sub.2 receptor, a method for determining whether a ligand which is not known to be capable of binding to the 5-HT.sub.2 receptor can bind to the 5-HT.sub.2 receptor, a method for detecting the presence of 5-HT.sub.2 receptor on the surface of a cell, and a method of screening drugs to identify drugs which specifically interact with, and bind to, the 5-HT.sub.2 receptor. The invention herein also concerns an antibody directed to the human 5-HT.sub.2 receptor, such as a monoclonal antibody directed to an epitope of the 5-HT.sub.2 receptor present on the surface of a cell and having an amino acid sequence included within the amino acid sequence shown in FIGS. 2A-2G.

摘要翻译： 本发明提供编码5-HT2受体的分离的核酸分子和作为人5-HT 2受体的分离的蛋白质。 本发明还提供了包含编码人5-HT2受体的DNA分子的载体和适于在细菌，酵母或哺乳动物细胞中表达5-HT2受体的载体。 另外，本发明提供了可用于检测编码5-HT2受体的核酸的DNA探针，一种不知道能够结合5-HT2受体的配体是否可以结合5-HT2受体的方法 受体，用于检测细胞表面上5-HT2受体的存在的方法，以及筛选药物以鉴定与5-HT2受体特异性相互作用并结合的药物的方法。 本发明还涉及针对人类5-HT2受体的抗体，例如针对细胞表面上存在的5-HT2受体的表位的单克隆抗体，并具有包含在氨基酸序列内的氨基酸序列 如图1和2所示。 2A-2G。

公开(公告)号：US5698571A

公开(公告)日：1997-12-16

申请号：US318330

申请日：1994-10-05

申请人： James E. Audia ,  Marlene L. Cohen ,  Kirk W. Johnson ,  Lee A. Phebus ,  Theresa Branchek

发明人： James E. Audia ,  Marlene L. Cohen ,  Kirk W. Johnson ,  Lee A. Phebus ,  Theresa Branchek

IPC分类号： A61K45/00 ,  A61K31/00 ,  A61K31/135 ,  A61K31/40 ,  A61K31/403 ,  A61K31/4045 ,  A61K31/445 ,  A61K31/454 ,  A61K31/4545 ,  A61K31/48 ,  A61P25/04 ,  A61P25/06 ,  A61P43/00 ,  A61K31/13 ,  A61K31/405

CPC分类号： A61K31/135 ,  A61K31/00 ,  A61K31/403 ,  A61K31/4045 ,  A61K31/454 ,  A61K31/48

摘要： The present invention provides a method for the treatment of migraine with compounds or compositions that are selective agonists of 5-HT.sub.1F receptors relative to other serotonin receptors that produce unwanted effects like vasoconstriction. The neurogenic meningeal extravasation that leads to the pain of migraine is inhibited.

摘要翻译： 本发明提供了一种治疗偏头痛的方法，其中化合物或组合物是相对于产生不想要的作用如血管收缩的其它5-羟色胺受体的5-HT1F受体的选择性激动剂。 导致偏头痛疼痛的神经源性脑膜外渗受到抑制。

公开(公告)号：US20060014242A1

公开(公告)日：2006-01-19

申请号：US10987856

申请日：2004-11-12

申请人： Christophe Gerald ,  Richard Weinshank ,  Mary Walker ,  Theresa Branchek

发明人： Christophe Gerald ,  Richard Weinshank ,  Mary Walker ,  Theresa Branchek

IPC分类号： C07K14/705 ,  C07H21/04 ,  A61K31/495

CPC分类号： G01N33/9406 ,  A01K2217/05 ,  A61K31/137 ,  A61K38/00 ,  C07K14/70571 ,  C12Q1/6883 ,  G01N2500/02

摘要： This invention provides methods of modifying feeding behavior, including increasing or decreasing food consumption, e.g., in connection with treating obesity, bulimia or anorexia. These methods involve administration of compounds are selective agonists or antagonists or the Y5 receptor. One such compound has the structure: In addition, this invention provides an isolated nucleic acid molecule encoding a Y5 receptor, an isolated Y5 receptor protein, vectors comprising an isolated nucleic acid molecule encoding a Y5 receptor, cells comprising such vectors, antibodies directed to the Y5 receptor, nucleic acid probes useful for detecting nucleic acid encoding Y5 receptors, antisense oligonucleotides complementary to any unique sequences of a nucleic acid molecule which encodes a Y5 receptor, and nonhuman transgenic animals which express DNA a normal or a mutant Y5 receptor.