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公开(公告)号：US20130302905A1
公开(公告)日：2013-11-14
申请号：US13885979
申请日：2011-11-11
申请人： Thomas Kalkbrenner , Helmut Lippert , Ingo Kleppe
发明人： Thomas Kalkbrenner , Helmut Lippert , Ingo Kleppe
IPC分类号： G01N21/64
CPC分类号： G01N21/6447 , G01N21/64 , G01N21/6428 , G01N21/6458 , G02B21/0076 , G02B21/16 , G02B21/367 , G02B27/58
摘要： A method for high-resolution luminescence microscopy of a sample marked with marking molecules that can be activated to excite particular luminescent radiation, including: repeated activation of a subset of the marking molecules to emit luminescent radiation; repeated imaging of the sample along a depth direction and with a predetermined optical resolution; and producing images from the repeated imaging. Locations of the marking molecules are determined with a spatial resolution that is increased above the predetermined optical resolution. Activation of the marking molecules can be through radiation introduced into multiple regions, each extending along a plane substantially perpendicular to the depth direction. The regions can be arranged so that the regions are behind one another and overlap only partially. Separate images of the sample may be recorded for activation in each of the regions in order to obtain depth information relating to the marking molecules from the separate images.
摘要翻译： 标记有可被激活以激发特定发光辐射的标记分子的样品的高分辨率发光显微镜的方法，包括：重复激活标记分子的子集以发射发光辐射; 沿深度方向和预定的光学分辨率重复成像样品; 并从重复成像产生图像。 标记分子的位置以高于预定的光学分辨率的空间分辨率来确定。 标记分子的激活可以是通过引入多个区域的辐射，每个区域沿着基本上垂直于深度方向的平面延伸。 这些区域可以被布置成使得区域彼此相邻并且仅部分重叠。 可以记录样品的分离图像以激活每个区域，以获得与分离图像相关的标记分子的深度信息。
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公开(公告)号：US20120281264A1
公开(公告)日：2012-11-08
申请号：US13498095
申请日：2010-09-17
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076 , G02B21/16 , G02B26/08 , G02B27/0911
摘要： A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.
摘要翻译： 显微镜系列包括照明装置，其沿着照明光束路径的照明轴线和垂直于照明轴线的横轴产生平面光片。 检测装置沿着检测光束路径的检测轴检测从样本区域发射的光。 照明和检测轴以及横轴和检测轴线以不等于零的角度相对定向。 光片发生器还产生旋转对称的光，并且包括用于沿横轴快速扫描样品区域的结构和控制。 照明装置包括具有第一散光活性光学元件的第二光片发生器，该光学元件具有用于产生静态光的至少一个散光透镜。 用于选择第一或第二光照片或两者的选择元件一起产生光线。
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公开(公告)号：USD648572S1
公开(公告)日：2011-11-15
申请号：US29391616
申请日：2011-05-11
申请人： Klaus Werner , Helmut Lippert
设计人： Klaus Werner , Helmut Lippert
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公开(公告)号：US08052105B2
公开(公告)日：2011-11-08
申请号：US12336097
申请日：2008-12-16
申请人： Helmut Lippert , Peter Reichert
发明人： Helmut Lippert , Peter Reichert
IPC分类号： F16M11/04
CPC分类号： H04R1/026 , H04R2209/027
摘要： A holder for a loudspeaker box onto which the loudspeaker box can be slipped. The holder includes a control handle and a clamping device actuatable without tools via the control handle.
摘要翻译： 扬声器盒可以滑动到其上的支架。 支架包括控制手柄和夹紧装置，可通过控制手柄无需工具即可启动。
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公开(公告)号：USD634737S1
公开(公告)日：2011-03-22
申请号：US29292061
申请日：2007-09-27
申请人： Helmut Lippert , Wolfgang Seidel
设计人： Helmut Lippert , Wolfgang Seidel
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公开(公告)号：USD600385S1
公开(公告)日：2009-09-15
申请号：US29294409
申请日：2008-01-09
申请人： Helmut Lippert
设计人： Helmut Lippert
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公开(公告)号：USD564117S1
公开(公告)日：2008-03-11
申请号：US29239046
申请日：2005-09-23
申请人： Helmut Lippert
设计人： Helmut Lippert
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公开(公告)号：US09201011B2
公开(公告)日：2015-12-01
申请号：US13885979
申请日：2011-11-11
申请人： Thomas Kalkbrenner , Helmut Lippert , Ingo Kleppe
发明人： Thomas Kalkbrenner , Helmut Lippert , Ingo Kleppe
CPC分类号： G01N21/6447 , G01N21/64 , G01N21/6428 , G01N21/6458 , G02B21/0076 , G02B21/16 , G02B21/367 , G02B27/58
摘要： A method for high-resolution luminescence microscopy of a sample marked with marking molecules that can be activated to excite particular luminescent radiation, including: repeated activation of a subset of the marking molecules to emit luminescent radiation; repeated imaging of the sample along a depth direction and with a predetermined optical resolution; and producing images from the repeated imaging. Locations of the marking molecules are determined with a spatial resolution that is increased above the predetermined optical resolution. Activation of the marking molecules can be through radiation introduced into multiple regions, each extending along a plane substantially perpendicular to the depth direction. The regions can be arranged so that the regions are behind one another and overlap only partially. Separate images of the sample may be recorded for activation in each of the regions in order to obtain depth information relating to the marking molecules from the separate images.
摘要翻译： 标记有可被激活以激发特定发光辐射的标记分子的样品的高分辨率发光显微镜的方法，包括：重复激活标记分子的子集以发射发光辐射; 沿深度方向和预定的光学分辨率重复成像样品; 并从重复成像产生图像。 标记分子的位置以高于预定的光学分辨率的空间分辨率来确定。 标记分子的激活可以是通过引入多个区域的辐射，每个区域沿着基本上垂直于深度方向的平面延伸。 这些区域可以被布置成使得区域彼此相邻并且仅部分重叠。 可以记录样品的分离图像以激活每个区域，以获得与分离图像相关的标记分子的深度信息。
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公开(公告)号：US08848268B2
公开(公告)日：2014-09-30
申请号：US13498095
申请日：2010-09-17
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076 , G02B21/16 , G02B26/08 , G02B27/0911
摘要： A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.
摘要翻译： 显微镜系列包括照明装置，其沿着照明光束路径的照明轴线和垂直于照明轴线的横轴产生平面光片。 检测装置沿着检测光束路径的检测轴检测从样本区域发射的光。 照明和检测轴以及横轴和检测轴线以不等于零的角度相对定向。 光片发生器还产生旋转对称的光，并且包括用于沿横轴快速扫描样品区域的结构和控制。 照明装置包括具有第一散光活性光学元件的第二光片发生器，该光学元件具有用于产生静态光的至少一个散光透镜。 用于选择第一或第二光照片或两者的选择元件一起产生光线。
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公开(公告)号：US20140254005A1
公开(公告)日：2014-09-11
申请号：US14350884
申请日：2012-10-06
申请人： Helmut Lippert , Tobias Kaufhold , Jörg Ritter
发明人： Helmut Lippert , Tobias Kaufhold , Jörg Ritter
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/16 , G02B21/367
摘要： A method for SPIM microscopy, wherein the sample is moved continuously, and a plurality of images are taken at time intervals by means of a detection arrangement during the movement. The image capture duration or exposure time is dimensioned such that the movement path of the sample lies within a predetermined resolution range of the detection objective. The speed of the sample movement is determined and set by the image capture duration or exposure time and/or the distortion of the point spread function generated by the sample movement of the sample. The image blur is corrected computationally by the respective image capture duration and the movement speed. A sharp image is generated in this way. The actual optical section thickness of the light sheet is determined from the light sheet thickness, and the movement speed is determined therefrom and from user settings.
摘要翻译： 一种用于SPIM显微镜的方法，其中样品连续移动，并且在运动期间通过检测装置以时间间隔拍摄多个图像。 图像捕获持续时间或曝光时间的尺寸使得样本的移动路径位于检测对象的预定分辨率范围内。 样品运动的速度由图像捕获持续时间或曝光时间确定和设置，和/或由样品的样品移动产生的点扩散函数的失真。 通过相应的图像捕获持续时间和移动速度计算地校正图像模糊。 以这种方式产生清晰的图像。 光片的实际光学部分厚度由光板厚度确定，并且根据用户设置确定移动速度。
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