公开(公告)号：US20140031238A1

公开(公告)日：2014-01-30

申请号：US13859667

申请日：2013-04-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John DAVIDSON

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

公开(公告)号：US20130288904A1

公开(公告)日：2013-10-31

申请号：US13859360

申请日：2013-04-09

Applicant: Life Technologies Corporation

Inventor： Earl HUBBELL ,  Jonathan SCHULTZ

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607

Abstract: A method for nucleic acid sequencing includes: disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array; exposing template polynucleotide strands to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.

Abstract translation: 用于核酸测序的方法包括：在传感器阵列的多个限定空间中设置多个模板多核苷酸链，测序引物和聚合酶; 将模板多核苷酸链暴露于一系列核苷酸物种流，该序列包含一系列随机流; 并且对于核苷酸物种的一系列流程中的每一个获得指示针对该特定流出现多少个核苷酸掺入以确定对应于模板多核苷酸链的核苷酸的预测序列的信号。