CELL-FREE SYSTEM FOR SYNTHESIS OF PROTEINS DERIVED FROM CULTURED MAMMALIAN CELLS
    41.
    发明申请
    CELL-FREE SYSTEM FOR SYNTHESIS OF PROTEINS DERIVED FROM CULTURED MAMMALIAN CELLS 有权
    用于合成从培养的哺乳动物细胞衍生的蛋白质的无细胞系统

    公开(公告)号:US20110300575A1

    公开(公告)日:2011-12-08

    申请号:US13211280

    申请日:2011-08-16

    IPC分类号: C12P21/00 C12N9/14

    CPC分类号: C12P21/02 C07K14/4702

    摘要: Prepared is an extract composition having an improved protein synthetic activity in a cell-free protein synthesis system using a mammalian cultured cell extract. An eukaryotic translation initiation factor and/or translational regulator are added to a cell-free protein synthesis system comprising an extract prepared from cultured mammalian cells and a template mRNA. These factors are one or more selected from the group consisting of eukaryotic translation initiation factors 4E (eIF4E), 2 (eIF2) and 2B (eIF2B), and eukaryotic translational regulator p97.

    摘要翻译: 制备的是在使用哺乳动物培养细胞提取物的无细胞蛋白质合成系统中具有改进的蛋白质合成活性的提取物组合物。 将真核翻译起始因子和/或翻译调节子添加到包含由培养的哺乳动物细胞和模板mRNA制备的提取物的无细胞蛋白质合成系统中。 这些因子是选自真核翻译起始因子4E(eIF4E),2(eIF2)和2B(eIF2B)以及真核翻译调节子p97中的一种或多种。

    Aromatic compound
    43.
    发明授权
    Aromatic compound 失效
    芳香族化合物

    公开(公告)号:US07842941B2

    公开(公告)日:2010-11-30

    申请号:US10574563

    申请日:2004-10-05

    IPC分类号: H01L35/24 C07D311/82

    CPC分类号: C07D307/91

    摘要: An aromatic compound of the following formula (1), (2), (5) or (6), wherein, Ar1 and Ar3 represent a tetra-valent aromatic hydrocarbon group or a tetra-valent heterocyclic group, and Ar2, Ar4, Ar5, Ar6 and Ar7 represent a tri-valent aromatic hydrocarbon group or a tri-valent heterocyclic group, A1 represents —Z1—, —Z2—Z3— or —Z4═Z5—, wherein Z1, Z2 and Z3 represent O, S or the like and Z4 and Z5 represent N, B, P or the like, X1, X2, X3, X4, X9, X10, X11, and X12 represent a halogen atom or the like.

    摘要翻译: 下式(1),(2),(5)或(6)的芳族化合物,其中Ar1和Ar3表示四元芳香族烃基或四价杂环基,Ar2,Ar4,Ar5 中,Ar 6和Ar 7表示3价芳香族烃基或3价杂环基,A1表示-Z 1 - ,Z 2 -Z 3 - 或-Z 4 = Z 5 - ,其中Z 1,Z 2和Z 3表示O,S或 Z 4和Z 5表示N,B,P等,X 1,X 2,X 3,X 4,X 9,X 10,X 11和X 12表示卤素原子等。

    Cell-free protein synthesis system for synthesizing glycoprotein
    44.
    发明申请
    Cell-free protein synthesis system for synthesizing glycoprotein 审中-公开
    用于合成糖蛋白的无细胞蛋白质合成系统

    公开(公告)号:US20090317862A1

    公开(公告)日:2009-12-24

    申请号:US11798760

    申请日:2007-05-16

    IPC分类号: C12P21/00

    CPC分类号: C12P21/005 C12P21/02

    摘要: Provided is a process for producing a protein with post-translational modification in a cell-free protein synthesis system having a higher protein synthetic activity and glycosylation capability. The process of the present invention comprises preparing a cell extract from cultured cells of an immortalized mammalian cell line that has enhanced protein secreting activity, and adding an mRNA encoding a glycoprotein to the cell extract.

    摘要翻译: 提供了在具有较高蛋白质合成活性和糖基化能力的无细胞蛋白质合成系统中产生具有翻译后修饰的蛋白质的方法。 本发明的方法包括从具有增强的蛋白质分泌活性的永生化哺乳动物细胞系的培养细胞制备细胞提取物,并向细胞提取物中加入编码糖蛋白的mRNA。

    Cell-free system for synthesis of proteins derived from cultured mammalian cells
    45.
    发明申请
    Cell-free system for synthesis of proteins derived from cultured mammalian cells 有权
    用于合成来自培养的哺乳动物细胞的蛋白质的无细胞系统

    公开(公告)号:US20070281337A1

    公开(公告)日:2007-12-06

    申请号:US11798759

    申请日:2007-05-16

    IPC分类号: C12P21/06 C12N5/08

    CPC分类号: C12P21/02 C07K14/4702

    摘要: Prepared is an extract composition having an improved protein synthetic activity in a cell-free protein synthesis system using a mammalian cultured cell extract. An eukaryotic translation initiation factor and/or translational regulator are added to a cell-free protein synthesis system comprising an extract prepared from cultured mammalian cells and a template mRNA. These factors are one or more selected from the group consisting of eukaryotic translation initiation factors 4E (eIF4E), 2 (eIF2) and 2B (eIF2B), and eukaryotic translational regulator p97.

    摘要翻译: 制备的是在使用哺乳动物培养细胞提取物的无细胞蛋白质合成系统中具有改进的蛋白质合成活性的提取物组合物。 将真核翻译起始因子和/或翻译调节子添加到包含由培养的哺乳动物细胞和模板mRNA制备的提取物的无细胞蛋白质合成系统中。 这些因子是选自真核翻译起始因子4E(eIF4E),2(eIF2)和2B(eIF2B)以及真核翻译调节子p97中的一种或多种。

    Aromatic compound
    46.
    发明申请
    Aromatic compound 失效
    芳香族化合物

    公开(公告)号:US20070063190A1

    公开(公告)日:2007-03-22

    申请号:US10574563

    申请日:2004-10-05

    CPC分类号: C07D307/91

    摘要: An aromatic compound of the following formula (1), (2), (5) or (6). wherein, Ar1 and Ar3 represent a tetra-valent aromatic hydrocarbon group or a tetra-valent heterocyclic group, and Ar2, Ar4, Ar5, Ar6 and Ar7 represent a tri-valent aromatic hydrocarbon group or a tri-valent heterocyclic group, A1 represents -Z1-, -Z2-Z3- or -Z4=Z5-, wherein Z1, Z2 and Z3 represent O, S or the like and Z4 and Z5 represent N, B, P or the like, X1, X2, X3, X4, X9, X10, X11, and X12 represent a halogen atom or the like.

    摘要翻译: 下式(1),(2),(5)或(6)的芳族化合物。 其中Ar 1和Ar 3代表4价芳族烃基或4价杂环基,Ar 2,Ar 2, SUP> 4,Ar 5,Ar 6和Ar 7表示3价芳香族烃基或3价芳香族烃基 杂环基,A 1表示-Z 1, - Z 2 - Z 3 - 或 - Z - 其中Z 1,Z 2和Z 3表示0至5个Z 5,其中Z 1,Z 2和Z 3表示 O,S等,Z 4和Z 5表示N,B,P等,X 1,X 2, X 3,X 3,X 4,X 9,X 10,X 10, 11和X 12表示卤素原子等。

    Optical transmission line for wavelength division multiplexed optical
signals
    47.
    发明授权
    Optical transmission line for wavelength division multiplexed optical signals 失效
    用于波分复用光信号的光传输线

    公开(公告)号:US5956440A

    公开(公告)日:1999-09-21

    申请号:US778140

    申请日:1997-01-02

    摘要: Wavelength division multiplexed optical signals with different wavelength dispersion are compensated for wavelength dispersion in a optical transmission line. Each multiplexed wavelength .lambda.1-.lambda.n is selected by band pass filters 11-1-11-n. The selected optical signal for each wavelength is independently compensated for the wavelength dispersion by wavelength dispersion compensation sections 12-1-12-n. The wavelength dispersion compensation section of each wavelength has a optical fiber having a dispersion value with polarity opposite to that of the optical transmission line. Zero dispersion is attained for each wavelength .lambda.1-.lambda.n as a whole, and transmission distance of wavelength division multiplexed optical signals can be extended.

    摘要翻译: 具有不同波长色散的波分复用光信号被补偿在光传输线中的波长色散。 每个复用波长λ1-lambda n由带通滤波器11-1-11-n选择。 通过波长色散补偿部分12-1-12-n独立地补偿每个波长的所选光信号的波长色散。 每个波长的波长色散补偿部分具有一个具有与光传输线的极性相反极性的色散值的光纤。 对于每个波长λ1-λn整体获得零色散,并且可以扩展波分复用光信号的传输距离。