公开(公告)号：US20070118917A1

公开(公告)日：2007-05-24

申请号：US11544942

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070094748A1

公开(公告)日：2007-04-26

申请号：US11544961

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070089188A1

公开(公告)日：2007-04-19

申请号：US11545029

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular consrtucts and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

摘要翻译： 本发明提供了分子结构及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子内的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。

公开(公告)号：US20050060769A1

公开(公告)日：2005-03-17

申请号：US10816410

申请日：2004-04-01

申请人： Larry Gilbertson

发明人： Larry Gilbertson

IPC分类号： C12N15/31 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N15/8213

摘要： A process to prepare a recombined transgenic Zea mays plant or plant cell from a first transgenic Zea mays plant cell, wherein the transgene in the recombinant plant or plant cell has an altered genetic structure relative to the genetic structure of the transgene in the first transgenic plant cell, due to homologous recombination-mediated transgene deletion.

摘要翻译： 从第一转基因玉米植物细胞制备重组转基因玉米植物或植物细胞的方法，其中重组植物或植物细胞中的转基因相对于第一转基因植物中转基因的遗传结构具有改变的遗传结构 由于同源重组介导的转基因缺失。