公开(公告)号：US08937214B2

公开(公告)日：2015-01-20

申请号：US12910377

申请日：2010-10-22

申请人： Larry Gilbertson ,  Jason Ward ,  Jianping Xu ,  Jon Lamb

发明人： Larry Gilbertson ,  Jason Ward ,  Jianping Xu ,  Jon Lamb

IPC分类号： C07K14/415 ,  A01H5/10 ,  C12N15/82

CPC分类号： C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

摘要： Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

摘要翻译： 提供包含单个基因座中包含的多个转基因的转基因植物。 在某些方面，可以从第一位点表达7个或更多个转基因。 提供了用于转化具有多个转基因的植物细胞的方法。 还提供了用于表达和增强植物中一种或多种转基因表达的方法。

公开(公告)号：US08829275B2

公开(公告)日：2014-09-09

申请号：US13558336

申请日：2012-07-25

申请人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

发明人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

IPC分类号： C12N15/113 ,  C12N15/82 ,  C12N15/84 ,  C12N5/10 ,  A01H1/04 ,  A01H1/02 ,  A01H5/00 ,  C12N9/10 ,  C12Q1/68

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12N9/1085 ,  C12N15/8205 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

公开(公告)号：US20120131699A1

公开(公告)日：2012-05-24

申请号：US13271666

申请日：2011-10-12

申请人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

发明人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N1/21 ,  C12N5/04 ,  C12N15/52 ,  C12N15/63

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12N9/1085 ,  C12N15/8205 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

公开(公告)号：US20110197311A1

公开(公告)日：2011-08-11

申请号：US13035916

申请日：2011-02-25

申请人： Larry Gilbertson ,  Xudong Ye

发明人： Larry Gilbertson ,  Xudong Ye

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00

CPC分类号： C12N15/8205 ,  C12N15/8202

摘要： The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

摘要翻译： 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US20110099672A1

公开(公告)日：2011-04-28

申请号：US12910377

申请日：2010-10-22

申请人： Larry Gilbertson ,  Joson Ward ,  Jianping Xu ,  Jon Lamb

发明人： Larry Gilbertson ,  Joson Ward ,  Jianping Xu ,  Jon Lamb

IPC分类号： A01H5/00

CPC分类号： C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

摘要： Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

摘要翻译： 提供包含单个基因座中包含的多个转基因的转基因植物。 在某些方面，可以从第一位点表达7个或更多个转基因。 提供了用于转化具有多个转基因的植物细胞的方法。 还提供了用于表达和增强植物中一种或多种转基因表达的方法。

公开(公告)号：US20090117539A1

公开(公告)日：2009-05-07

申请号：US11399000

申请日：2006-04-05

申请人： Larry Gilbertson ,  Scott C. Johnson ,  David K. Kovalic ,  Tracey L. Reynolds ,  James F. Rice, JR. ,  James K. Roberts

发明人： Larry Gilbertson ,  Scott C. Johnson ,  David K. Kovalic ,  Tracey L. Reynolds ,  James F. Rice, JR. ,  James K. Roberts

IPC分类号： C12Q1/68 ,  C12N5/06

CPC分类号： C12Q1/6895

摘要： The present invention provides methods of improving DNA sequences for gene suppression mediated by double-stranded RNA, and constructs and transgenic organisms containing such improved DNA sequences.

摘要翻译： 本发明提供了改进由双链RNA介导的基因抑制的DNA序列的方法，以及含有这种改良的DNA序列的构建体和转基因生物。

公开(公告)号：US20080066202A1

公开(公告)日：2008-03-13

申请号：US11856958

申请日：2007-09-18

申请人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

发明人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

IPC分类号： C12N15/84 ,  C12N15/82

CPC分类号： C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。

公开(公告)号：US20080064107A1

公开(公告)日：2008-03-13

申请号：US11779739

申请日：2007-07-18

申请人： Larry Gilbertson ,  Xudong Ye

发明人： Larry Gilbertson ,  Xudong Ye

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/02 ,  C12P21/04

CPC分类号： C12N15/8205 ,  C12N15/8202

摘要： The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

摘要翻译： 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US20070113302A1

公开(公告)日：2007-05-17

申请号：US11545099

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070089192A1

公开(公告)日：2007-04-19

申请号：US11545086

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.