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公开(公告)号：US20070118918A1
公开(公告)日：2007-05-24
申请号：US11545071
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070118917A1
公开(公告)日：2007-05-24
申请号：US11544942
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070094748A1
公开(公告)日：2007-04-26
申请号：US11544961
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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4.发明申请公开(公告)号：US20070089188A1
公开(公告)日：2007-04-19
申请号：US11545029
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular consrtucts and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子结构及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子内的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US20070028322A1
公开(公告)日：2007-02-01
申请号：US11542046
申请日：2006-10-03
申请人： Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
发明人： Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
CPC分类号： C12N15/8254 , A01H5/10 , C12Q1/6895
摘要： Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译： 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物，其活性导致植物或植物产物中赖氨酸增加。
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公开(公告)号：US20050250938A1
公开(公告)日：2005-11-10
申请号：US10660097
申请日：2003-09-11
申请人： Alan Kriz , Michael Luethy , Dale Voyles
发明人： Alan Kriz , Michael Luethy , Dale Voyles
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/8216
摘要： Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
摘要翻译： 公开了在单子叶植物（包括玉米）中表达转基因的方法和组合物。 在本发明中，通过使用来自Coix属植物的单子叶 - 同源序列来避免转基因沉默。 这些转基因序列中包括Coix启动子，增强子，编码序列和终止子。 基于同源性的基因沉默可以限制或有效地消除转基因表达，玉米衍生的转基因的合适替代物对于在玉米中表达是期望的。
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公开(公告)号：US20070089195A1
公开(公告)日：2007-04-19
申请号：US11545105
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070089194A1
公开(公告)日：2007-04-19
申请号：US11545098
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070089190A1
公开(公告)日：2007-04-19
申请号：US11545082
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070089186A1
公开(公告)日：2007-04-19
申请号：US11544962
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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