公开(公告)号：US20070083947A1

公开(公告)日：2007-04-12

申请号：US11544928

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.