公开(公告)号：US5097025A

公开(公告)日：1992-03-17

申请号：US444591

申请日：1989-12-01

申请人： Philip N. Benfey ,  Nam-Hai Chua

发明人： Philip N. Benfey ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  C12N15/83

CPC分类号： C12N15/8216 ,  C12N15/8223 ,  C12N15/8234

摘要： Five subdomains of the CaMV35S promoter are provided that cause tissue specific and/or developmentally regulated expression of chimeric genes in plants. These subdomains act as promoters for use in transformed plant cells, seeds and transgenic plants. Some of the subdomains require fusion to domain A for expression. Subdomains B2, B3, B4, and B5 exhibit expression when fused to the minimal promoter sequence in mature plants whereas only B2 and B3 confer expression in seeds and only B2, B3 and B4 confer expression at the seedling stage of development. The combination of subdomains B4 and B5 confers expression at all stages of development as does the B1+TGACG motif combination when each combination is fused with the minimal promoter sequence. The nucleotide sequence and DNA molecule that function as the enhancers are provided.

摘要翻译： 提供CaMV35S启动子的五个亚结构域，其导致植物中嵌合基因的组织特异性和/或发育调控表达。 这些子结构域用作转化植物细胞，种子和转基因植物中的启动子。 一些子域需要融合到域A进行表达。 子结构域B2，B3，B4和B5在成熟植物中与最小启动子序列融合时显示出表达，而仅B2和B3赋予种子表达，只有B2，B3和B4在幼苗发育阶段赋予表达。 子结构域B4和B5在开发的所有阶段赋予表达，如B1 + TGACG基序组合，当每个组合与最小启动子序列融合时。 提供用作增强子的核苷酸序列和DNA分子。