公开(公告)号：US20070099256A1

公开(公告)日：2007-05-03

申请号：US11262244

申请日：2005-10-28

申请人： Narayan Sundararajan ,  Handong Li

发明人： Narayan Sundararajan ,  Handong Li

IPC分类号： C12Q1/37 ,  G06F19/00

CPC分类号： G01N33/6842 ,  G01N33/6848 ,  Y02A90/26

摘要： Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of peptides using Surface Enhanced Raman Spectroscopy (SERS) and Raman spectroscopy. Additional embodiments provide strategies for chemically derivatizing post-translational modifications and detecting the chemically derivatized products using SERS. Applications of embodiments of the present invention include proteome wide modification profiling and analyses with applications in disease diagnosis, prognosis and drug efficacy studies, enzymatic activity profiling and assays.

摘要翻译： 本发明的实施方案提供了使用表面增强拉曼光谱（SERS）和拉曼光谱法检测，鉴定，区分和定量肽的修饰状态的装置和方法。 另外的实施方案提供了用于化学衍生翻译后修饰并使用SERS检测化学衍生化产物的策略。 本发明实施方案的应用包括在疾病诊断，预后和药物功效研究，酶活性分析和测定中的应用的蛋白质组广泛修饰分析和分析。