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    Method for the selective enrichment and labeling of phosphorproteins
    1.
    发明申请
    Method for the selective enrichment and labeling of phosphorproteins 有权
    磷蛋白选择性富集和标记方法

    公开(公告)号：US20080081782A1

    公开(公告)日：2008-04-03

    申请号：US11529541

    申请日：2006-09-29

    申请人： Handong Li ,  Narayan Sundararajan

    发明人： Handong Li ,  Narayan Sundararajan

    IPC分类号： A61K38/16 ,  C07K14/475

    CPC分类号： C07K1/13 ,  C07K7/08

    摘要： The embodiments of the invention relate to a method for the introduction of a labeling structure such as a fluorescent molecules or a Raman tags to a compound. Imidazole functionalized resins or polymers are used to selectively immobilize phosphocompounds without protecting the carboxylic groups. Relying on the pKa difference between amines and hydrazides and carrying out the reaction in a slightly acidic buffer, all of the amines are protected by protonation while the hydrazides react with the phosphate imidazolide to form a phosphoramidate bond.

    摘要翻译： 本发明的实施方案涉及将化合物引入诸如荧光分子或拉曼标签的标记结构的方法。 咪唑官能化树脂或聚合物用于选择性地固定磷酸化合物而不保护羧基。 依赖于胺和酰肼之间的pKα差异并在微酸性缓冲液中进行反应，所有的胺都被质子化保护，而酰肼与磷酸咪唑化物反应形成氨基磷酸酯键。

    Enzymatic and chemical method for increased peptide detection sensitivity using surface enhanced raman scattering (SERS)
    2.
    发明申请
    Enzymatic and chemical method for increased peptide detection sensitivity using surface enhanced raman scattering (SERS) 审中-公开
    使用表面增强拉曼散射（SERS）增加肽检测灵敏度的酶和化学方法

    公开(公告)号：US20080081340A1

    公开(公告)日：2008-04-03

    申请号：US11529574

    申请日：2006-09-29

    申请人： Anil Patwardhan ,  Handong Li ,  Narayan Sundararajan

    发明人： Anil Patwardhan ,  Handong Li ,  Narayan Sundararajan

    IPC分类号： G01N33/53 ,  G01N33/553 ,  C12P21/06 ,  C12M3/00

    CPC分类号： G01N33/68 ,  C12P21/06 ,  G01N33/54373

    摘要： The sensitivity of surface enhanced Raman spectroscopy to silver nano-particle/peptide aggregates is increased by prior treatment of the peptides. According to a first type of embodiment, an enzyme such as Glu-C is used for protein(s) digestion based on the enzyme's ability to cleave proteins at a selected location having a negative charge, such as at aspartic acid and glutamic acid. This type of digestion is used to derive a higher proportion of positively charged component peptides sequences as compared to the component peptides sequences obtained by standard tryptic digestion of protein(s). According to a second type of embodiment, methyl-esterification of peptides suppresses the negative charge contributions of portions of the peptides such as aspartic acid, glutamic acid, and the C-terminus. Both types of embodiments result in increased binding affinity of the resulting component sequence peptides with negatively charged nano-particles such as silver nano-particles. According to yet other embodiments, the first and second types of embodiments can be combined for further sensitivity increase.

    摘要翻译： 表面增强拉曼光谱对银纳米颗粒/肽聚集体的敏感性通过先前处理肽而增加。 根据第一类型的实施方案，基于酶在具有负电荷的选定位置（例如天冬氨酸和谷氨酸）切割蛋白质的能力，使用诸如Glu-C的酶进行蛋白质消化。 与通过蛋白质的标准胰蛋白酶消化获得的成分肽序列相比，这种类型的消化用于导出更高比例的带正电荷的组分肽序列。 根据第二类型的实施方案，肽的甲基酯化抑制了肽部分的负电荷贡献，例如天冬氨酸，谷氨酸和C末端。 两种类型的实施方案导致所得组分序列肽与带负电荷的纳米颗粒如银纳米颗粒的结合亲和力增加。 根据其他实施例，可以组合第一和第二类型的实施例以进一步提高灵敏度。

    Assay platforms and detection methodology using surface enhanced Raman scattering (SERS) upon specific biochemical interactions
    4.
    发明申请
    Assay platforms and detection methodology using surface enhanced Raman scattering (SERS) upon specific biochemical interactions 审中-公开
    使用表面增强拉曼散射（SERS）进行特异性生化相互作用的测定平台和检测方法

    公开(公告)号：US20080003576A1

    公开(公告)日：2008-01-03

    申请号：US11477675

    申请日：2006-06-30

    申请人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

    发明人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

    IPC分类号： C12Q1/68 ,  G01N33/555

    CPC分类号： G01N33/585 ,  C12Q1/6816 ,  G01N21/658 ,  G01N33/54373 ,  C12Q2563/155 ,  C12Q2565/632

    摘要： The embodiments of the invention are directed to a SERS cluster comprising a capture particle that is at least partially surrounded by analyte molecules, wherein both the capture particle and the analyte molecules surrounding the capture particle are at least partially surrounded by enhancer particles, wherein a majority of the analyte molecules are either sandwiched between capture and enhancer particles or located between junctions of the enhancer particles. The embodiments of the invention also relate to methods of manufacturing and detecting the SERS cluster. The embodiments of the invention also relate to a SERS active particle comprising a tag molecule comprising a Raman active compound and a probe or a linker having a specific biochemical binding capability and to a method for detecting of a target molecule using a SERS active particle having a tag molecule comprising a Raman active compound and a probe or a linker.

    摘要翻译： 本发明的实施方案涉及包含被分析物分子至少部分包围的捕获颗粒的SERS簇，其中捕获颗粒和捕获颗粒周围的分析物分子至少部分地被增强子颗粒包围，其中多数 分析物分子被夹在捕获和增强剂颗粒之间或位于增强剂颗粒的结之间。 本发明的实施例还涉及制造和检测SERS簇的方法。 本发明的实施方案还涉及包含包含拉曼活性化合物的标签分子和具有特定生物化学结合能力的探针或接头的SERS活性颗粒以及使用具有下列化合物的SERS活性颗粒检测靶分子的方法： 标签分子，其包含拉曼活性化合物和探针或接头。

    Phosphopeptide detection and surface enhanced Raman spectroscopy
    8.
    发明申请
    Phosphopeptide detection and surface enhanced Raman spectroscopy 审中-公开
    磷酸肽检测和表面增强拉曼光谱

    公开(公告)号：US20080158558A1

    公开(公告)日：2008-07-03

    申请号：US11647690

    申请日：2006-12-28

    申请人： Handong Li ,  Narayan Sundararajan

    发明人： Handong Li ,  Narayan Sundararajan

    IPC分类号： G01J3/44

    CPC分类号： G01N21/658 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/573 ,  G01N33/587 ,  G01N33/6842

    摘要： Raman-active molecules having specific affinity for phosphorylated peptides and proteins are provided. The Raman-active affinity molecules contain a Raman active group capable of providing a detectable spectrum. The affinity molecules act as tags or reporter molecules and are useful, for example in detecting the presence of a phosphorylated residue in a peptide or protein through the use of SERS spectroscopy. The affinity molecules provide the ability to detect and quantify phosphatase and kinase activities.

    摘要翻译： 提供了对磷酸化肽和蛋白质具有特异性亲和力的拉曼活性分子。 拉曼活性亲和分子含有能够提供可检测光谱的拉曼活性基团。 亲和分子用作标签或报告分子，并且可用于例如通过使用SERS光谱法检测肽或蛋白质中磷酸化残基的存在。 亲和分子提供检测和定量磷酸酶和激酶活性的能力。

    Method for the selective enrichment and labeling of phosphorproteins
    9.
    发明授权
    Method for the selective enrichment and labeling of phosphorproteins 有权
    磷蛋白选择性富集和标记方法

    公开(公告)号：US08859734B2

    公开(公告)日：2014-10-14

    申请号：US11529541

    申请日：2006-09-29

    申请人： Handong Li ,  Narayan Sundararajan

    发明人： Handong Li ,  Narayan Sundararajan

    IPC分类号： C07K7/04 ,  C07K1/13

    CPC分类号： C07K1/13 ,  C07K7/08

    摘要： The embodiments of the invention relate to a method for the introduction of a labeling structure such as a fluorescent molecules or a Raman tags to a compound. Imidazole functionalized resins or polymers are used to selectively immobilize phosphocompounds without protecting the carboxylic groups. Relying on the pKa difference between amines and hydrazides and carrying out the reaction in a slightly acidic buffer, all of the amines are protected by protonation while the hydrazides react with the phosphate imidazolide to form a phosphoramidate bond.

    摘要翻译： 本发明的实施方案涉及将化合物引入诸如荧光分子或拉曼标签的标记结构的方法。 咪唑官能化树脂或聚合物用于选择性地固定磷酸化合物而不保护羧基。 依赖于胺和酰肼之间的pKα差异并在微酸性缓冲液中进行反应，所有的胺都被质子化保护，而酰肼与磷酸咪唑化物反应形成氨基磷酸酯键。