公开(公告)号：US06888138B2

公开(公告)日：2005-05-03

申请号：US10868907

申请日：2004-06-17

Applicant: Takashi Ohshima ,  Hiroshi Makino ,  Hiroshi Toyama ,  Hiroyuki Shinada

Inventor： Takashi Ohshima ,  Hiroshi Makino ,  Hiroshi Toyama ,  Hiroyuki Shinada

IPC: G01N23/225 ,  G01Q30/02 ,  H01J37/04 ,  H01J37/244 ,  H01J37/28

CPC classification number: H01J37/244 ,  H01J37/28 ,  H01J2237/2605

Abstract: It was hard for conventional SEMs to take measurements at a high speed and take accurate measurements when an insulator exists between an object to probe and the detector, because the conventional SEMs used a continuous electron beam. Also, it was impossible to apply voltage to the sample during the measurement of current. By pulse-modulating the electron beam and extracting a high-frequency signal component from the sample, new SEM equipment disclosed herein detects electrons absorbed in the sample at a high speed and with precision. Precise and high-speed absorption current measurements can be achieved. High-functionality inspection apparatus can be provided.

Abstract translation: 传统的扫描电镜很难在高速下进行测量，并且当物体与探针和检测器之间存在绝缘体时进行精确的测量，因为传统的扫描电镜使用连续的电子束。 此外，在测量电流期间不可能对样品施加电压。 通过脉冲调制电子束并从样品中提取高频信号分量，本文公开的新的SEM设备以高速和精确的方式检测样品中吸收的电子。 可以实现精确和高速吸收电流测量。 可以提供高功能检查装置。

公开(公告)号：US20050045820A1

公开(公告)日：2005-03-03

申请号：US10868907

申请日：2004-06-17

Applicant: Takashi Ohshima ,  Hiroshi Makino ,  Hiroshi Toyama ,  Hiroyuki Shinada

Inventor： Takashi Ohshima ,  Hiroshi Makino ,  Hiroshi Toyama ,  Hiroyuki Shinada

IPC: G01N23/225 ,  G01Q30/02 ,  H01J37/04 ,  H01J37/244 ,  H01J37/28

CPC classification number: H01J37/244 ,  H01J37/28 ,  H01J2237/2605

Abstract: It was hard for conventional SEMs to take measurements at a high speed and take accurate measurements when an insulator exists between an object to probe and the detector, because the conventional SEMs used a continuous electron beam. Also, it was impossible to apply voltage to the sample during the measurement of current. By pulse-modulating the electron beam and extracting a high-frequency signal component from the sample, new SEM equipment disclosed herein detects electrons absorbed in the sample at a high speed and with precision. Precise and high-speed absorption current measurements can be achieved. High-functionality inspection apparatus can be provided.

Abstract translation: 传统的扫描电镜很难在高速下进行测量，并且当物体与探针和检测器之间存在绝缘体时进行精确的测量，因为传统的扫描电镜使用连续的电子束。 此外，在测量电流期间不可能对样品施加电压。 通过脉冲调制电子束并从样品中提取高频信号分量，本文公开的新的SEM设备以高速和精确的方式检测样品中吸收的电子。 可以实现精确和高速吸收电流测量。 可以提供高功能检查装置。

公开(公告)号：US5965718A

公开(公告)日：1999-10-12

申请号：US145376

申请日：1998-09-01

Applicant: Kyriacos C. Nicolaou ,  Floris VanDelft ,  Seijiro Hosokawa ,  Sanghee Kim ,  Tianhu Li ,  Takashi Ohshima ,  Jeff Pfefferkorn ,  Dionisios Vourloumis ,  Jin-You Xu ,  Nicolas Winssinger

Inventor： Kyriacos C. Nicolaou ,  Floris VanDelft ,  Seijiro Hosokawa ,  Sanghee Kim ,  Tianhu Li ,  Takashi Ohshima ,  Jeff Pfefferkorn ,  Dionisios Vourloumis ,  Jin-You Xu ,  Nicolas Winssinger

IPC: C07H15/26 ,  A61K31/34 ,  A61K31/4164 ,  A61K31/422 ,  A61K31/427 ,  A61K31/443 ,  A61K31/7028 ,  A61K31/7048 ,  A61P35/00 ,  A61P43/00 ,  C07D493/08 ,  C07F7/18 ,  C07H15/24 ,  A61K31/70 ,  C07D307/77

CPC classification number: C07D493/08

Abstract: Sarcodictyin A and B, eleutherobin, and bioactive analogs thereof synthesized using solid phase and solution phase chemistries. The synthetic method employs an attachment of common precursors, e.g., compounds 1880 or 200, on a solid support for generating conjugates 230 and 240, followed by standard chemical manipulations. A combinatorial library of sarcodictyins and eletherobin analogs was constructed with modified C-8 ester, C-15 ester and C-4 ketal functionalities and was screened for activity with respect to tubulin polymerization and cytotoxic activity against tumor cells, including Taxol-resistant lines. Compounds 600, 610, 630, 660-700, 730, 760, 850, and 920 were identified to be of equal or superior biological activities as compared to their corresponding natural product.

Abstract translation: Sarcodictyin A和B，eleutherobin及其使用固相和溶液相化学合成的生物活性类似物。 合成方法使用常用前体（例如化合物1880或200）在固体支持物上的附着，用于产生共轭物230和240，随后进行标准化学操作。 用修饰的C-8酯，C-15酯和C-4缩酮官能团构建了链霉菌素和eletherobin类似物的组合文库，并筛选了关于微管蛋白聚合和抗肿瘤细胞（包括耐紫杉醇系）的细胞毒活性的活性。 与其相应的天然产物相比，化合物600,610,630,660-700,730,760,850和920被鉴定为具有相同或优异的生物活性。