公开(公告)号：US20140142291A1

公开(公告)日：2014-05-22

申请号：US14045565

申请日：2013-10-03

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： Hartmut Duefel ,  Dieter Heindl ,  Carina Horn ,  Thomas Meier ,  Rainer Schmuck

IPC: C07H19/207

CPC classification number: C07H19/207 ,  C12P19/32

Abstract: The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.

Abstract translation: 本公开内容涉及烟酰胺腺嘌呤二核苷酸NAD / NADH和烟酰胺腺嘌呤二核苷酸磷酸NADP / NADPH的稳定类似物的酶合成，即所谓的“carba-NAD”，即NAD / NADH或NADP / NADPH的类似物，分别包含 碳环糖代替核糖。

公开(公告)号：US20130289251A1

公开(公告)日：2013-10-31

申请号：US13923631

申请日：2013-06-21

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： Andreas Gallusser ,  Dieter Heindl ,  Michael Schraeml ,  Christoph Seidel ,  Herbert von der Eltz

IPC: C07K19/00

CPC classification number: C07K19/00 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/626 ,  C07K2317/92 ,  C07K2317/94

Abstract: A binding agent of the Formula A-a′:a-S-b:b′-B:X(n), wherein A as well as B is a monovalent binder, a′:a as well as b:b′ is a binding pair wherein a′ and a do not interfere with the binding of b to b′ and vice versa, S is a spacer of at least 1 nm in length, :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a′, a, b, b′ or S, (n) is an integer and at least 1, - represents a covalent bond, and the linker a-S-b has a length of 6 to 100 nm. Also disclosed are methods of producing such binding agent and certain uses thereof.

Abstract translation: 式A-a'：aSb：b'-B：X（n）的结合剂，其中A以及B是单价键合物，a：a以及b：b'是结合对，其中 a'和a不干扰b与b'的结合，反之亦然，S是长度至少为1nm的间隔物：X表示与共价键或通过结合对键合到至少一个 a'，a，b，b'或S，（n）为整数且至少为1， - 表示共价键，连接体aSb的长度为6〜100nm。 还公开了生产这种结合剂及其某些用途的方法。