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    Localized Excess Protons and Methods of Making and Using Same
    5.
    发明申请
    Localized Excess Protons and Methods of Making and Using Same 审中-公开
    本地化的过量质子及其使用方法

    公开(公告)号：US20170009357A1

    公开(公告)日：2017-01-12

    申请号：US15202214

    申请日：2016-07-05

    申请人： James Weifu Lee

    发明人： James Weifu Lee

    IPC分类号： C25B1/06 ,  C23F1/14 ,  C12P19/40 ,  C25B1/10

    CPC分类号： C25B1/06 ,  C12P19/32 ,  C12Y306/03014 ,  C23F1/14 ,  C25B1/10 ,  Y02E60/366 ,  Y02P20/129

    摘要： Localized excess protons are created with an open-circuit water electrolysis process using a pair of anode and cathode electrodes for a special excess proton production and proton-utilization system to treat a substrate material plate/film by forming and using an excess protons-substrate-hydroxyl anions capacitor-like system. The technology enables protonation and/or proton-driven oxidation of plate/film and/or membrane materials in a pure water environment. The present invention represents a remarkable clean “green chemistry” technology that does not require the use of any conventional acid chemicals including nitric and sulfuric acids for the said industrial applications. The application of localized excess protons provides a special energy recycling and renewing technology function to extract latent heat including molecular thermal motion energy at ambient temperature for generating local proton motive force (equivalent to Gibbs free energy) to do useful work such as driving ATP synthesis and proton-driven oxidation of certain substrate metal atoms.

    摘要翻译： 使用一对阳极和阴极用于特殊的过量质子生成和质子利用系统的开放水电解方法产生局部过量质子，以通过形成和使用过量的质子 - 衬底 - 羟基阴离子电容器系统。 该技术可在纯水环境中使板/膜和/或膜材料进行质子化和/或质子驱动氧化。 本发明代表了非常清洁的“绿色化学”技术，其不需要使用任何常规的酸性化学品，包括用于所述工业应用的硝酸和硫酸。 局部过量质子的应用提供了一种特殊的能量回收和更新技术功能，用于提取包括分子热运动能在环境温度下的潜热，以产生局部质子动力（相当于吉布斯自由能）来进行有用的工作，如驱动ATP合成和 某些底物金属原子的质子驱动氧化。

    METHODS OF USING ACYL-COA SYNTHETASE FOR BIOSYNTHETIC PRODUCTION OF ACYL-COAS
    7.
    发明申请
    METHODS OF USING ACYL-COA SYNTHETASE FOR BIOSYNTHETIC PRODUCTION OF ACYL-COAS 审中-公开
    ACYL-COA合成酶用于生产亚油酸的方法

    公开(公告)号：US20160273014A1

    公开(公告)日：2016-09-22

    申请号：US15033711

    申请日：2014-11-03

    申请人： CONAGEN INC.

    发明人： Chen Hui ,  Wang Hongxue ,  Yu Xiaodan

    IPC分类号： C12P19/32

    CPC分类号： C12P19/32 ,  C12P7/6436

    摘要： A biosynthetic method of making carboxyl CoA from long-chain carboxylic acid including expressing an ACS in a cellular system, feeding a long-chain carboxylic acid to the cellular system, growing the cellular system in a medium, and producing carboxyl CoA.

    摘要翻译： 一种从长链羧酸制备羧基CoA的生物合成方法，包括在细胞系统中表达ACS，向细胞系统中加入长链羧酸，在培养基中培养细胞系，并生产羧基CoA。

    METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL
    8.
    发明申请
    METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL 审中-公开

    公开(公告)号：US20160053288A1

    公开(公告)日：2016-02-25

    申请号：US14700103

    申请日：2015-04-29

    申请人： Genomatica, Inc.

    发明人： Mark J. BURK ,  Christophe H. SCHILLING ,  Anthony P. BURGARD ,  John D. TRAWICK

    IPC分类号： C12P7/18

    CPC分类号： C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

    摘要： The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

    THERMOSTABLE NUCLEASE
    9.
    发明申请
    THERMOSTABLE NUCLEASE 审中-公开
    热稳定核

    公开(公告)号：US20160053242A1

    公开(公告)日：2016-02-25

    申请号：US14867175

    申请日：2015-09-28

    申请人： UNIVERSITE LAVAL

    发明人： Maurice BOISSINOT ,  Karel Boissinot ,  Sandra Isabel ,  Lu-E Shi

    IPC分类号： C12N9/22 ,  C12P19/34

    CPC分类号： C12N9/22 ,  A23L27/23 ,  A61K31/7088 ,  C12P19/32 ,  C12P19/34 ,  C12Y301/04

    摘要： A heat-stable nuclease found in Y. enterocolitica subsp. Palearctica, named Nucyep, is active in broad spectrum conditions. The gene for Nucyep was sequenced in a strain Y. enterocolitica subsp. palearctica, cloned, and expressed in E. coli, and then purified and characterized. The molecular weight of this enzyme is about 30 to 32 kDa. The translation product, Nucyep1, is biologically active. The purified Nucyep1 exhibits non-specific nuclease activity, being able to degrade various nucleic acids, including RNA, single-stranded DNA (ssDNA) and linear or circular double-stranded DNA (dsDNA). This enzyme is active in a wide range of temperatures, from 0 to 100° C. The enzyme is active in a wide range of pH values from 3.6 to 9.9, and keeps greater than 75% of the activity at pH 7.24. This enterobacterial nuclease has unique levels of intrinsic resistance to heat, and is active under a large spectrum of conditions.

    摘要翻译： 在肠杆菌属亚种中发现的热稳定核酸酶 Paleoctica，名为Nucyep，在广泛的条件下是活跃的。 将Nucyep的基因在小肠结肠炎菌株亚种中测序。 在大肠杆菌中克隆并表达，然后纯化和表征。 该酶的分子量为约30至32kDa。 翻译产品Nucyep1具有生物活性。 纯化的Nucyep1显示非特异性核酸酶活性，能够降解各种核酸，包括RNA，单链DNA（ssDNA）和线性或环状双链DNA（dsDNA）。 该酶在0至100℃的宽范围的温度范围内是有活性的。酶在3.6至9.9的宽范围的pH值范围内是有活性的，并且在pH 7.24下保持大于75％的活性。 这种肠杆菌核酸酶具有独特的固有抗热性，并且在大范围的条件下是有活性的。

    MICROORGANISM FOR PRODUCTION OF CHEMICALS DERIVED FROM ACETYL-COA
    10.
    发明申请
    MICROORGANISM FOR PRODUCTION OF CHEMICALS DERIVED FROM ACETYL-COA 审中-公开
    用于生产从乙酰辅酶A衍生的化学物质的微生物

    公开(公告)号：US20150218607A1

    公开(公告)日：2015-08-06

    申请号：US14428611

    申请日：2014-01-23

    申请人： MITSUI CHEMICALS, INC.

    发明人： Anjali Madhavan ,  Ryota Fujii ,  Tomonori Hidesaki ,  Su Sun Chong

    IPC分类号： C12P19/32 ,  C12P13/14

    CPC分类号： C12P19/32 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P1/04 ,  C12P13/14 ,  C12Y401/03024 ,  C12Y602/01009

    摘要： Disclosed is an acetyl-CoA-producing microorganism, which is obtained by imparting malate thiokinase and malyl-CoA lyase enzymatic activities to a microorganism having none of the following (a), (b), (c) or (d), without imparting any of (a), (b), (c) or (d), or, even when one or more of (a), (b), (c) or (d) are imparted, not allowing the functions thereof to be exerted: (a) a carbon dioxide fixation cycle having an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate, (b) a carbon dioxide fixation cycle having an enzymatic reaction from acetyl-CoA and CO2 to pyruvate, (c) a carbon dioxide fixation cycle having an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA or glutaconyl-CoA or (d) a carbon dioxide fixation cycle having an enzymatic reaction from CO2 to formate.

    摘要翻译： 公开了通过赋予没有以下（a），（b），（c）或（d）所示的微生物的苹果酸硫激酶和丙烯酰辅酶A裂解酶酶活性而获得的乙酰辅酶A微生物，而不赋予 （a），（b），（c）或（d）中的任何一种，或者即使赋予（a），（b），（c）或（d）中的一种或多种， （a）具有从丙二酰辅酶A到丙二酸半醛或3-羟丙酸酯的酶反应的二氧化碳固定循环，（b）具有从乙酰辅酶A和CO 2到丙酮酸的酶反应的二氧化碳固定循环，（c ）具有从巴豆酰CoA和CO 2到乙基丙二酰CoA或戊烯酰辅酶A的酶反应的二氧化碳固定循环，或（d）具有从CO 2到甲酸盐的酶反应的二氧化碳固定循环。