公开(公告)号：US06245519B1

公开(公告)日：2001-06-12

申请号：US09502966

申请日：2000-02-11

申请人： Steven T. Brentano ,  Sherrol H. McDonough ,  Norman C. Nelson

发明人： Steven T. Brentano ,  Sherrol H. McDonough ,  Norman C. Nelson

IPC分类号： C12Q168

CPC分类号： C12Q1/6813 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/107 ,  C12Q2525/117 ,  C12Q2563/103 ,  C12Q2561/108 ,  C12Q2537/113 ,  C12Q2537/162

摘要： The present invention features compositions and methods that are useful for storing labeled detection probes and detecting whether a target nucleic acid sequence is present in a sample. Preferred compositions are made up of a detection probe containing a label susceptible to a chemical or enzymatic alteration and a protection probe that protects the label from alteration and/or decreases the ability of the detection probe to inhibit nucleic acid amplification. Such compositions can be used, for example, to stabilize a detection probe label and to prevent a detection probe from hybridizing prematurely to amplified or target nucleic acid.

摘要翻译： 本发明的特征在于可用于存储标记的检测探针并检测样品中是否存在靶核酸序列的组合物和方法。 优选的组合物由含有对化学或酶促改变敏感的标记的检测探针和保护标记免受改变和/或降低检测探针抑制核酸扩增能力的保护探针组成。 这样的组合物可以用于例如稳定检测探针标记并防止检测探针过早与扩增或靶核酸杂交。

公开(公告)号：US5948899A

公开(公告)日：1999-09-07

申请号：US462823

申请日：1995-06-05

申请人： Lyle J. Arnold, Jr. ,  Norman C. Nelson

发明人： Lyle J. Arnold, Jr. ,  Norman C. Nelson

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  C07H21/04

CPC分类号： G01N33/58 ,  C12Q1/6816 ,  C12Q1/6823 ,  G01N33/5306 ,  G01N33/532 ,  G01N33/533 ,  G01N33/542

摘要： Improved homogenous diagnostic assay methods and labels for detecting an analyte in a medium when the analyte is a member of a specific binding pair. The methods and labels provide procedures for reducing background and increasing sensitivity. The binding partner of the analyte is labeled with a substance, the stability of which detectably changes whenever said analyte is bound as a member of the specific binding pair. In a closely related system, the analyte is labeled with a substance susceptible to differential degradation depending on whether or not the analyte is bound as a member of its specific binding pair. After incubation and selective degradation or chemical or biochemical alteration, the amount of analyte bound is detected by measuring either the stability change or the extent of degradation of the label. In a particular system, chemiluminescent acridinium ester labeled probes are used in a homogenous hybridization assay format for sensitively detecting the presence of complement any target polynucleotide sequences.

摘要翻译： 当分析物是特异性结合对的成员时，改进的同源诊断测定方法和用于检测培养基中分析物的标记。 方法和标签提供了降低背景和提高灵敏度的程序。 分析物的结合配偶体用物质标记，当所述分析物作为特异性结合对的成员结合时，其稳定性可检测地改变。 在紧密相关的系统中，根据分析物是否作为其特异性结合对的成员结合，分析物被易受差异降解敏感的物质标记。 孵育和选择性降解或化学或生物化学改变后，通过测量标签的稳定性变化或降解程度来检测分析物的结合量。 在特定系统中，化学发光的吖啶酯标记的探针以均质杂交测定形式使用，以敏感地检测补体存在任何靶多核苷酸序列。

公开(公告)号：US4060723A

公开(公告)日：1977-11-29

申请号：US649404

申请日：1976-01-15

申请人： Norman C. Nelson

发明人： Norman C. Nelson

IPC分类号： F21L4/00 ,  H01H15/02 ,  H01H3/40 ,  F21V23/04

CPC分类号： H01H15/02 ,  F21L15/06

摘要： A flashlight assembly including a cylindrical housing defining a battery compartment and a separated lamp compartment with a circuit controlling switch located entirely on the outside of the cylindrical enclosure, the lamp compartment lying in a separate part of the cylindrical enclosure which is threadedly connected to the main portion of the enclosure, there being an electrical connector between the switch unit and the lamp and socket unit which extends from the switch on the outside of the enclosure to the interior of the lamp compartment and remains entirely outside the battery compartment.

摘要翻译： 一种手电筒组件，其包括限定电池室的圆柱形壳体和具有完全位于圆柱形外壳的外侧上的电路控制开关的分离的灯室，灯室位于圆柱形外壳的分离部分中，螺旋连接到主体 外壳的一部分，在开关单元和灯和插座单元之间存在电连接器，其从壳体的外部的开关延伸到灯室的内部并且完全保持在电池室的外部。