公开(公告)号：US20190241961A1

公开(公告)日：2019-08-08

申请号：US16333177

申请日：2017-09-15

申请人： Dyax Corp.

发明人： Daniel J. Sexton ,  Malini Viswanathan ,  Ryan Faucette ,  Tripti Gaur

IPC分类号： C12Q1/6883 ,  C12Q1/686 ,  C12Q1/6813 ,  C12Q1/6851

CPC分类号： C12Q1/6883 ,  C12Q1/6813 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/502 ,  G01N2800/224

摘要： Provided herein are methods and kits for analyzing a biological sample obtained from a subject having, suspected of having, or being at risk for a disease associated with the contact activation system.

公开(公告)号：US20180306683A1

公开(公告)日：2018-10-25

申请号：US15925600

申请日：2018-03-19

申请人： Fluidigm Corporation

发明人： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

IPC分类号： G01N1/28 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/14

CPC分类号： G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

摘要： Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20180276332A1

公开(公告)日：2018-09-27

申请号：US15926977

申请日：2018-03-20

申请人： Cellular Research, Inc.

发明人： Jue Fan ,  Christina Fan ,  David Rosenfeld

IPC分类号： G06F19/12 ,  C12Q1/6876 ,  G06F15/18 ,  G06F19/20

CPC分类号： G16B5/00 ,  C12Q1/6813 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  G06N20/00 ,  G16B25/00 ,  C12Q2537/165 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2535/00

摘要： Disclosed herein include methods and systems for identifying multiplet expression profiles. A plurality of synthetic multiplet expression profiles can be generated from a plurality of expression profiles. An expression profile can be identified as an expression for a singlet or a multiplet using a machine learning model trained using the plurality of synthetic multiplet (e.g., doublet) expression profiles.

公开(公告)号：US20180265912A1

公开(公告)日：2018-09-20

申请号：US15853055

申请日：2017-12-22

申请人： Rutgers, The State University of New Jersey

发明人： Bin Tian ,  Dinghai Zheng

IPC分类号： C12Q1/6806 ,  C07H21/02 ,  C40B50/00 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12N9/22 ,  C12N9/00 ,  C07H19/06

CPC分类号： C12Q1/6806 ,  C07H19/06 ,  C07H21/02 ,  C12N9/22 ,  C12N9/93 ,  C12N2310/11 ,  C12N2310/3181 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2525/173 ,  C12Q2600/158 ,  C12Q2600/166 ,  C40B50/00 ,  G16B30/00 ,  G16B30/10 ,  C12Q2521/327 ,  C12Q2525/191

摘要： The present invention relates to modified 3′ region extraction and deep sequencing of polyadenylated RNA to identify a poly(A) site in a reference, as well as to calculate poly(A) tail length.

公开(公告)号：US20180202004A1

公开(公告)日：2018-07-19

申请号：US15858703

申请日：2017-12-29

申请人： Oncology Venture ApS

发明人： Steen KNUDSEN

IPC分类号： C12Q1/6886 ,  A61K9/127 ,  A61K33/24 ,  A61K45/06 ,  A61P35/00 ,  C12Q1/6813

CPC分类号： C12Q1/6886 ,  A61K9/127 ,  A61K33/24 ,  A61K45/06 ,  A61P35/00 ,  C12Q1/6813 ,  C12Q2600/136 ,  C12Q2600/142 ,  C12Q2600/158

摘要： The present invention features methods, devices, and kits for detecting a level of one or more biomarkers in a patient having cancer or determining the responsiveness of a patient having cancer to a treatment, such as treatment with a secretory phospholipase A2 (sPLA2) hydrolysable, cisplatin-containing liposome. The invention further includes methods of treating a patient having cancer by administering, e.g., the liposome.

公开(公告)号：US10000806B2

公开(公告)日：2018-06-19

申请号：US14720511

申请日：2015-05-22

申请人： Dow AgroSciences LLC

发明人： Tina Marie Kaiser ,  Stephen Novak

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/686 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2537/16 ,  C12Q2545/114 ,  C12Q2561/109

摘要： Disclosed herein are methods for determining if a contaminating integration of a nucleotide sequence is present in a set of nucleic acids. Further disclosed herein are methods for determining the copy number/zygosity of a nucleic acid sequence of interest. The methods disclosed herein may be performed using quantitative PCR.

公开(公告)号：US09952126B2

公开(公告)日：2018-04-24

申请号：US13781313

申请日：2013-02-28

申请人： Fluidigm Corporation

发明人： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C Norris ,  Dominique Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC分类号： C12P19/34 ,  G01N1/28 ,  C12Q1/68 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC分类号： G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

摘要： Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US09926591B2

公开(公告)日：2018-03-27

申请号：US15044886

申请日：2016-02-16

申请人： Tracesa Ltd.

发明人： Dominic Patrick Joseph McCann ,  Kevin John Forbes ,  Edyta Lam ,  Geoffrey Colin Maitland ,  Alexander Bismarck

IPC分类号： E21B49/00 ,  C12Q1/68 ,  E21B43/26 ,  C09K8/80 ,  C40B30/02 ,  G01N33/28

CPC分类号： C12Q1/6813 ,  C09K8/805 ,  C12Q1/68 ,  C12Q1/686 ,  C40B30/02 ,  E21B43/26 ,  G01N33/2882 ,  C12Q2563/155 ,  C12Q2563/185

摘要： A fluid identification system comprising a plurality of particles, each particle encapsulating therein at least one tracer material having an identifiable DNA, the at least one tracer material being encapsulated by an encapsulation material, wherein the particles are adapted to retain the at least one tracer material in an encapsulated form after exposure of the particles to a temperature of at least 75° C. and/or a pressure of at least 1000 psi (6.9×106 N/m2).

公开(公告)号：US09856527B2

公开(公告)日：2018-01-02

申请号：US15162263

申请日：2016-05-23

申请人： GEN-PROBE INCORPORATED

发明人： Daniel L. Kacian ,  Kenneth A. Browne

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q1/6813 ,  C12Q1/6865 ,  C12Q2545/101 ,  C12Q2537/143

摘要： Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

公开(公告)号：US20170369952A1

公开(公告)日：2017-12-28

申请号：US15625494

申请日：2017-06-16

申请人： AdvaTect Diagnostics, LLC

发明人： Dennis G. HOOPER ,  John S. Sutton

IPC分类号： C12Q1/68 ,  G01N33/569

CPC分类号： C12Q1/6895 ,  C12Q1/6813 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2600/142 ,  G01N33/56961 ,  G01N2469/10 ,  G01N2800/26 ,  G01N2800/50

摘要： This invention relates to methods and compositions for identifying microbial DNA in the tissues or body fluid samples of patients. More particularly, the invention relates to two-step polymerase chain reaction based methods for identifying microbial DNA in the tissues or body fluid samples of patients, and compositions therefor. Microbial DNA can also be quantified using the methods described herein.