公开(公告)号：US20190256870A1

公开(公告)日：2019-08-22

申请号：US16383412

申请日：2019-04-12

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  A61K38/46 ,  C12N9/22 ,  C12N15/113 ,  C12N15/70 ,  A01H6/46 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  A01K67/027 ,  C12N15/74

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10358659B2

公开(公告)日：2019-07-23

申请号：US16201853

申请日：2018-11-27

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/113 ,  C12N15/63 ,  C12N9/22 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10351878B2

公开(公告)日：2019-07-16

申请号：US16201855

申请日：2018-11-27

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  C12N15/113 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46 ,  A01H6/46 ,  A01K67/027 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10337029B2

公开(公告)日：2019-07-02

申请号：US16201848

申请日：2018-11-27

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： A61K38/46 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/113 ,  A01K67/027 ,  C12N15/90 ,  C12N15/74 ,  C12N15/70 ,  C12N9/22 ,  A01H6/46 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20190062790A1

公开(公告)日：2019-02-28

申请号：US15915020

申请日：2018-03-07

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  James Harrison Doudna Cate ,  Wendell Lim ,  Lei Qi ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  A01H6/46 ,  A01K67/027 ,  A61K38/46 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20190002922A1

公开(公告)日：2019-01-03

申请号：US16136165

申请日：2018-09-19

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  James Harrison Doudna Cate ,  Wendell Lim ,  Lei Qi ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  A01K67/027 ,  C12N9/22 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N15/70 ,  C12N15/74 ,  A61K38/46

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10113167B2

公开(公告)日：2018-10-30

申请号：US15138604

申请日：2016-04-26

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/70 ,  C12N15/90 ,  C12N15/63 ,  C12N15/74 ,  A61K38/46 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20180282764A1

公开(公告)日：2018-10-04

申请号：US15803424

申请日：2017-11-03

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Martin Jinek ,  Emmanuelle Charpentier ,  Krzysztof Chylinski ,  James Harrison Doudna Cate ,  Wendell Lim ,  Lei Qi ,  Jennifer A. Doudna

IPC分类号： C12N15/90 ,  A61K38/46 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US10077445B2

公开(公告)日：2018-09-18

申请号：US15138604

申请日：2016-04-26

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/70 ,  C12N15/90 ,  C12N15/63 ,  C12N15/74 ,  A61K38/46 ,  A61K48/00

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20180251793A1

公开(公告)日：2018-09-06

申请号：US15981807

申请日：2018-05-16

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： A01K67/027 ,  C12N9/22 ,  C12N15/74 ,  C12N15/70 ,  C12N15/90 ,  C12N15/11 ,  C12N15/63 ,  C12N15/10 ,  C12N15/113 ,  A01H6/46

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.