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公开(公告)号：US20220383051A1
公开(公告)日：2022-12-01
申请号：US17771116
申请日：2020-10-29
Applicant: Keygene N.V.
Abstract: A plant selection apparatus has a storage means for storing a plurality of image datasets of plants and associated plant information including at least one of genotype information of the plants, phenotype information of the plants, and pedigree information of the plants. A selection unit preselects a subset of the plants. A display device displays the image datasets of the subset of the plants. A tracking unit generates observation information including information on which of the subset of the plants is observed, and the storage means is configured for storing the observation information. A training unit trains a classifier based on the observation information, the input selection, and the plant information including said at least one of the genotype information, the phenotype information, and the pedigree information.
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公开(公告)号：US20220298329A1
公开(公告)日：2022-09-22
申请号：US17837904
申请日：2022-06-10
Applicant: Keygene N.V.
Inventor： Raymond Jozef Maurinus HULZINK , Stefan John WHITE
IPC: C08L5/04 , C12Q1/6806 , C12N15/10
Abstract: The invention pertains to a method for isolating a nucleic acid, wherein the nucleic acid is stabilized in a hydrogel. The hydrogel can be dissolved to release the nucleic acid without breaking the molecule. A preferred hydrogel is alginate. The invention further concerns a method for sequencing the nucleic acid and a composition comprising the hydrogel and the nucleic acid.
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公开(公告)号：US20220106607A1
公开(公告)日：2022-04-07
申请号：US17535297
申请日：2021-11-24
Applicant: Keygene N.V.
Inventor： Charles Joseph UNDERWOOD , Diana RIGOLA , Peter Johannes VAN DIJK , Rik Hubertus Martinus OP DEN CAMP , Michael Eric SCHRANZ , Catharina Adriana VIJVERBERG
IPC: C12N15/82 , C07K14/415
Abstract: The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provides parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.
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公开(公告)号：US11284577B2
公开(公告)日：2022-03-29
申请号：US16491470
申请日：2018-03-07
Applicant: PureCircle USA Inc. , KeyGene N.V. , The Coca-Cola Company
Inventor： Avetik Markosyan , Seong Siang Ong , Runchun Jing , Tengfang Huang , Stephen Ezra Schauer , Fayaz Khazi , Indra Prakash , Alec Hayes
Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
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公开(公告)号：US20210324393A1
公开(公告)日：2021-10-21
申请号：US17316758
申请日：2021-05-11
Applicant: Keygene N.V.
Inventor： Paul BUNDOCK , Michiel Theodoor Jan DE BOTH , Frank LHUISSIER
IPC: C12N15/82
Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
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Patent Agency Ranking
公开(公告)号：US20210202035A1
公开(公告)日：2021-07-01
申请号：US17199164
申请日：2021-03-11
Applicant: KEYGENE N.V.
IPC: G16B30/00 , C40B30/04 , C12Q1/6827 , C12N15/10 , C12Q1/6874 , C12Q1/6809 , C12Q1/6806 , C12Q1/6883 , C12Q1/6858
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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公开(公告)号：US10988807B2
公开(公告)日：2021-04-27
申请号：US16539119
申请日：2019-08-13
Applicant: Keygene N.V.
IPC: C12Q1/6874 , C12Q1/6827 , C12Q1/683
Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.
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公开(公告)号：US20200270626A1
公开(公告)日：2020-08-27
申请号：US16811393
申请日：2020-03-06
Applicant: KEYGENE N.V.
Inventor： Paul BUNDOCK
Abstract: The invention pertains to the targeted alteration of a duplex DNA in a cell, whereby two site-specific nucleases generate an indel, such that the open reading frame is not altered after the second indel. The invention further pertains to the use of such nucleases for the targeted alteration of an open reading frame in duplex DNA and a kit of parts for use in a method of the invention. Using the method of the invention, novel plants were obtained having an improved herbicide resistance. The invention therefore also concerns plants having improved herbicide resistance due to the expression of an altered ALS protein.
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公开(公告)号：US10738319B2
公开(公告)日：2020-08-11
申请号：US15880825
申请日：2018-01-26
Applicant: Keygene N.V.
Inventor： Anne Deslattes Mays , Marieke Helena Adriana Van Hulten , Shital Anilkumar Dixit , Martin De Vos , Jesse David Munkvold , Matthew Vitabile Dileo
Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant products that can be obtained by the method according to the invention.
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公开(公告)号：US20200045916A1
公开(公告)日：2020-02-13
申请号：US16449329
申请日：2019-06-21
Applicant: Keygene N.V.
Inventor： Jeroen STUURMAN
Abstract: The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.
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