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公开(公告)号：US20230407366A1
公开(公告)日：2023-12-21
申请号：US18029493
申请日：2021-10-06
Applicant: KEYGENE N.V.
IPC: C12Q1/6806 , C12Q1/48 , C12N9/22 , C12N15/10
CPC classification number: C12Q1/6806 , C12Q1/485 , C12N2310/20 , C12N15/1093 , C12N9/22
Abstract: The invention pertains to a method for labelling a target nucleic acid fragment using a combination of a site-specific nuclease and a reverse transcriptase. The labelling results in the addition of a specific nucleotide sequence to at least one free 3′-end of the target nucleic acid fragment. The invention further relates to a method for determining the sequence of the target nucleic acid fragment as well as construct and kit for use in the method of the invention.
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公开(公告)号：US20220033879A1
公开(公告)日：2022-02-03
申请号：US17297859
申请日：2019-11-27
Applicant: KEYGENE N.V.
Inventor： Stefan John WHITE , René Cornelis Josephus HOGERS
IPC: C12Q1/6806 , C12N15/11 , C12N9/22
Abstract: The current invention pertains to a method for the enrichment of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. The invention further pertains to the use of the enriched target nucleic acid fragments for preparing an adapter ligated target nucleic acid fragment and for sequencing the target nucleic acid fragment.
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公开(公告)号：US11107551B2
公开(公告)日：2021-08-31
申请号：US14898005
申请日：2014-06-13
Applicant: Keygene N.V.
Inventor： Jacob Bernhard Buntjer , José Lúcio Lima Guerra , Timotheus Gerardus Doeswijk , Remco Van Berloo , Niek Bouman
IPC: G16B20/00 , G16B40/00 , A01H1/04 , C12Q1/6876
Abstract: The present invention provides a method for improving at least one phenotypic trait of interest in subsequent generation(s) of a population of individuals, preferably crop plants or cattle. Particularly, the method identifies the combination of at least three individuals that gives, upon subsequent intercrossing, the highest estimated probability of improving the at least one phenotypic trait of interest in the subsequent generation(s). Also provided is a computer-readable medium comprising instructions for performing the method.
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公开(公告)号：US10978175B2
公开(公告)日：2021-04-13
申请号：US16517411
申请日：2019-07-19
Applicant: KEYGENE N.V.
IPC: G16B30/00 , C40B30/04 , C12Q1/6827 , C12N15/10 , C12Q1/6874 , C12Q1/6809 , C12Q1/6806 , C12Q1/6883 , C12Q1/6858 , C40B30/10
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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公开(公告)号：US10961545B2
公开(公告)日：2021-03-30
申请号：US16399655
申请日：2019-04-30
Applicant: Keygene N.V.
Inventor： Anne Deslattes Mays , Marieke Helena Adriana Van Hulten , Shital Anilkumar Dixit , Martin De Vos , Jesse David Munkvold , Matthew Vitabile Dileo
IPC: C12N15/82
Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
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Patent Agency Ranking
公开(公告)号：US10422001B2
公开(公告)日：2019-09-24
申请号：US15683063
申请日：2017-08-22
Applicant: Keygene N.V.
IPC: C12Q1/6874 , C12Q1/6827 , C12Q1/683
Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.
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公开(公告)号：US20190249186A1
公开(公告)日：2019-08-15
申请号：US16399655
申请日：2019-04-30
Applicant: Keygene N.V.
Inventor： Anne DESLATTES MAYS , Marieke Helena Adriana VAN HULTEN , Shital Anilkumar DIXIT , Martin DE VOS , Jesse David MUNKVOLD , Matthew Vitabile DILEO
IPC: C12N15/82
CPC classification number: C12N15/8273
Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
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公开(公告)号：US20190225657A1
公开(公告)日：2019-07-25
申请号：US16302949
申请日：2017-05-19
Applicant: Keygene N.V.
IPC: C07K14/415 , A01H6/46 , A01H1/08
Abstract: The present disclosure provides a modified CenH3 protein that, when present in a plant, allows the plant to be used as a haploid inducer line for plant breeding purposes. Polynucleotides encoding such modified CenH3 proteins, chimeric genes and vectors comprising such polynucleotides, host cells, and plants comprising such polynucleotides, chimeric genes or vectors are also provided. Additionally, methods for making such plants as well as methods for producing haploid or doubled haploid plants using such plants are disclosed.
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公开(公告)号：US10308952B2
公开(公告)日：2019-06-04
申请号：US14377842
申请日：2013-02-18
Applicant: Keygene N.V.
Inventor： Anne Deslattes Mays , Marieke Helena Adriana Van Hulten , Shital Anilkumar Dixit , Martin De Vos , Jesse David Munkvold , Matthew Vitabile Dileo
Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
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公开(公告)号：US20190147977A1
公开(公告)日：2019-05-16
申请号：US16178344
申请日：2018-11-01
Applicant: Keygene N.V.
IPC: G16B30/00 , C12Q1/6874 , C12Q1/6806 , C40B30/04 , C12Q1/6883 , C12N15/10 , C12Q1/6858 , C12Q1/6827 , C12Q1/6809
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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