公开(公告)号：US20060193748A1

公开(公告)日：2006-08-31

申请号：US11177505

申请日：2005-07-11

申请人： Yu-Chong Tai ,  Jun Xie ,  Jason Shih ,  Terry Lee ,  Yunan Miao

发明人： Yu-Chong Tai ,  Jun Xie ,  Jason Shih ,  Terry Lee ,  Yunan Miao

IPC分类号： G01N30/02 ,  H01J49/00

CPC分类号： G01N30/34 ,  G01N30/16 ,  G01N30/6095 ,  G01N30/7266

摘要： A microfluidic system with on-chip pumping which can be used for liquid chromatography and also electrospray ionization mass spectrometry and which provides improved efficiency, better integration with sensors, improved portability, reduced power consumption, and reduced cost. The system can include (A) a main chip comprising: a substrate having a front face and a back face; a chromatography column on the front face of said substrate, wherein said column has an inlet and an outlet; an electrospray ionization (ESI) nozzle on the front face of said substrate, wherein said nozzle has an inlet and an outlet, and wherein the inlet of the nozzle is microfluidically coupled to the outlet of the column; one or more pump systems on the front face of said substrate comprising a pump chamber, one or more electrodes, and an outlet microfluidically coupled to the inlet of said column; and (B) a reservoir chip comprising a front surface and a back surface, wherein the reservoir chip has one or more cavities in the back surface which when disposed next to the front surface of the main chip extends the volume of the pump chamber of one of the pump system. Microfabrication can be used to prepare the chips, which can be assembled with a cover and inserted into a testing jig for electronic control and mass spectral analysis. Peptide separations are demonstrated which compete with present commercial systems.

摘要翻译： 具有片上泵浦的微流体系统，其可用于液相色谱和电喷雾离子化质谱，并且其提供更高的效率，更好地与传感器的集成，改进的便携性，降低的功率消耗和降低的成本。 该系统可以包括（A）主芯片，其包括：具有正面和背面的基板; 所述基板前表面上的色谱柱，其中所述色谱柱具有入口和出口; 在所述基板的前表面上的电喷雾离子化（ESI）喷嘴，其中所述喷嘴具有入口和出口，并且其中所述喷嘴的入口微流体耦合到所述塔的出口; 所述衬底的前表面上的一个或多个泵系统包括泵室，一个或多个电极和微流体耦合到所述塔的入口的出口; 和（B）包括前表面和后表面的储存器芯片，其中所述储存器芯片在所述后表面中具有一个或多个空腔，当所述储存器芯片靠近所述主芯片的前表面设置时，所述泵室的体积将一个 的泵系统。 可以使用微加工来制备芯片，其可以与盖组装并插入用于电子控制和质谱分析的测试夹具中。 展示了与现有商业系统竞争的肽分离。

公开(公告)号：US20060034933A1

公开(公告)日：2006-02-16

申请号：US10535955

申请日：2003-11-17

申请人： Jun Xie ,  Yan Bai ,  YiMu Yi ,  Guang Yang ,  ChangGong Zhang

发明人： Jun Xie ,  Yan Bai ,  YiMu Yi ,  Guang Yang ,  ChangGong Zhang

IPC分类号： A61K9/14 ,  A61K31/56

CPC分类号： A61K31/19 ,  A61K9/19

摘要： The invention discloses a freeze-dried nanometer powder injection of ursolic acid and a preparing method thereof. It major composes of active material of ursolic acid, complex vehicle of nanometer powder, excipient of freeze-drying. Compared to other popular preparations, its advantages are high target application of liver, concentrate on the affects, increase curative effect, decrease side effect, high dispersity and stability. It is prepared through dissolving ursolic acid as active raw material in medical organic solvent, adding complex vehicle of bean lecithin and stearic acid, stirring while heating until reaction completed, adding distilled water, adding excipient, stirring while heating until reaction completed, filtering with microporous membrane and freeze-drying the filtrate. Its average particle size is 209.5 mm, drug loading is 25.2%. it is for inhibiting and treating liver cancer, decreasing expression of p53, bcl-2 and topo-II.

摘要翻译： 本发明公开了一种冻干纳米粉末注射熊果酸及其制备方法。 它主要由熊果酸的活性物质，纳米粉末的复合载体，冷冻干燥的赋形剂组成。 与其他流行的制剂相比，其优点是肝脏靶向应用较高，注意力集中在影响上，提高疗效，降低副作用，分散性和稳定性高。 通过将熊果酸作为活性原料溶解在医用有机溶剂中，加入豆卵磷脂和硬脂酸的复合载体，加热搅拌直到反应完成，加入蒸馏水，加入赋形剂，加热搅拌直到反应完成，用微孔过滤 膜并冷冻干燥滤液。 其平均粒径为209.5毫米，药物载量为25.2％。 用于抑制和治疗肝癌，降低p53，bcl-2和topo-II的表达。

公开(公告)号：US20050051489A1

公开(公告)日：2005-03-10

申请号：US10917257

申请日：2004-08-11

申请人： Yu-Chong Tai ,  Qing He ,  Jun Xie ,  Changlin Pang ,  Terry Lee

发明人： Yu-Chong Tai ,  Qing He ,  Jun Xie ,  Changlin Pang ,  Terry Lee

IPC分类号： G01N30/60 ,  G01N30/64 ,  B01D15/08

CPC分类号： H05K3/284 ,  G01N30/6047 ,  G01N30/6095 ,  G01N2030/645 ,  G03F1/80 ,  G01N2030/625

摘要： Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, frits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals. A self-aligned channel-strengthening technique increases pressure rating of the microfluidic system, allowing it to withstand the high pressure normally used in high performance liquid chromatography (HPLC). On-chip sample injection, separation, and detection of mixture of anions in water is successfully demonstrated using ion-exchange nano-LC.

摘要翻译： 根据本发明的实施方案涉及集成在片上的填充柱纳米液相色谱（nano-LC）系统及其制造和使用方法。 微制造的芯片包括柱状物，玻璃料/过滤器，注射器和检测器，以与传统上用于形成集成电路的那些兼容的方法制造。 该柱可以填充有用于各种不同固定相的载体，以允许不同形式的纳米LC的性能，包括但不限于反相，正相，吸附，尺寸排阻，亲和力和离子色谱。 交叉通道注射器在柱入口处注入纳升/皮升体积的样品塞。 集成在色谱柱出口处的电化学/电导率传感器测量分离信号。 自对准的通道加强技术提高了微流体系统的压力等级，使其能够承受通常用于高效液相色谱（HPLC）的高压。 使用离子交换纳米LC成功地证明了在水中阴离子的混合物的片上样品注入，分离和检测。