公开(公告)号：US20100029954A1

公开(公告)日：2010-02-04

申请号：US12583513

申请日：2009-08-20

申请人： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

发明人： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC分类号： C07D403/06

CPC分类号： C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

摘要： Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

公开(公告)号：US20100029017A1

公开(公告)日：2010-02-04

申请号：US12220939

申请日：2008-07-29

申请人： Zhenjun Diwu ,  Timothy Dubrovsky ,  Barnaby Abrams ,  Jianjun He ,  Chunmei Wei ,  Jinfang Liao

发明人： Zhenjun Diwu ,  Timothy Dubrovsky ,  Barnaby Abrams ,  Jianjun He ,  Chunmei Wei ,  Jinfang Liao

IPC分类号： G01N33/536 ,  C07K16/18

CPC分类号： G01N33/533 ,  G01N33/582

摘要： The present invention provides fluorescent biopolymers that are amino acid polymers conjugated to multiple mono-chlorinated 3-carbonyl-7-hydroxy-coumarin dyes, and methods of their use. The fluorescent biopolymers containing multiple mono-chlorinated 3-carbonyl-7-hydroxy-coumarin dyes generally exhibit superior fluorescence properties compared to biopolymers labeled with multiple non-chlorinated hydroxycoumarin dyes. The fluorescent biopolymers of the invention in which the amino acid polymer is an antigen-specific antibody are particularly useful for analyzing cells by flow cytometers that are equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.

摘要翻译： 本发明提供荧光生物聚合物，其是与多种单氯化3-羰基-7-羟基香豆素染料缀合的氨基酸聚合物，以及它们使用的方法。 与使用多种非氯化羟基香豆素染料标记的生物聚合物相比，含有多种单氯化3-羰基-7-羟基香豆素染料的荧光生物聚合物通常表现出优异的荧光特性。 其中氨基酸聚合物是抗原特异性抗体的本发明的荧光生物聚合物特别可用于通过流式细胞仪分析细胞，由流式细胞仪配备紫激光作为激发源，因为它们在405nm处的强吸收和高荧光量子 产量。

公开(公告)号：US20080254498A1

公开(公告)日：2008-10-16

申请号：US12040753

申请日：2008-02-29

申请人： Zhenjun Diwu ,  Jianjun He ,  Jinfang Liao

发明人： Zhenjun Diwu ,  Jianjun He ,  Jinfang Liao

IPC分类号： C12Q1/02 ,  C07D311/82 ,  C07D493/10

CPC分类号： G01N33/84 ,  C07C229/24 ,  C07C2603/24 ,  C07D219/06 ,  C07D311/90 ,  C07D493/10 ,  G01N33/533

摘要： Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.

摘要翻译： 用于制备荧光金属离子指示剂的荧光染料，荧光指示剂本身，以及使用荧光指示剂检测，鉴别和定量金属阳离子。

公开(公告)号：US20050250152A1

公开(公告)日：2005-11-10

申请号：US11182122

申请日：2005-07-15

申请人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

发明人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

IPC分类号： G01N33/532 ,  C07D239/91 ,  C07D239/94 ,  C07D249/08 ,  C07D263/60 ,  C07D265/22 ,  C07D277/66 ,  C07D401/04 ,  G01N33/58 ,  C12Q1/68 ,  C07H21/04 ,  C08B37/00

CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-21-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

摘要翻译： 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 1 -C 21 - 亚烷基）氨基，氨基，羧基，氰基，卤素 ，羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基， 卤素，羟基，硝基，苯基，磺基，或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。