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77 条记录 (耗时 0.022 秒)

    METHOD FOR PRODUCING MONATIN
    73.
    发明申请
    METHOD FOR PRODUCING MONATIN 审中-公开
    生产MONATIN的方法

    公开(公告)号：US20120295314A1

    公开(公告)日：2012-11-22

    申请号：US13455381

    申请日：2012-04-25

    申请人： Masakazu Sugiyama ,  Yasuaki Takakura ,  Mika Moriya ,  Yusuke Hagiwara ,  Eri Tabuchi

    发明人： Masakazu Sugiyama ,  Yasuaki Takakura ,  Mika Moriya ,  Yusuke Hagiwara ,  Eri Tabuchi

    IPC分类号： C12P17/10 ,  C07H21/00 ,  C12N9/10

    CPC分类号： C12N9/88 ,  C12N9/1096 ,  C12N9/78 ,  C12P17/10

    摘要： The present invention provides a method of producing 2R,4R-Monatin with a good yield using inexpensive L-Trp rather than expensive D-Trp as a stating material. Specifically, the present invention provides a method for producing 2R,4R-Monatin or a salt thereof, comprising: (1) contacting L-tryptophan with a deamination enzyme to form indole-3-pyruvate; (2) contacting the indole-3-pyruvate and pyruvate with an aldolase to form 4R-IHOG; and (3) contacting the 4R-IHOG with a D-aminotransferase in the presence of a D-amino acid to form the 2R,4R-Monatin; and the like. In (3), it is preferable to use a D-aminotransferase having no or low ability to form D-tryptophan from indole-3-pyruvate.

    摘要翻译： 本发明提供使用廉价的L-Trp而不是昂贵的D-Trp作为说明材料，以高产率制备2R，4R-莫纳甜的方法。 具体而言，本发明提供了制备2R，4R-莫纳他嗪或其盐的方法，其包括：（1）使L-色氨酸与脱氨酶接触以形成吲哚-3-丙酮酸盐; （2）使吲哚-3-丙酮酸和丙酮酸与醛缩酶接触以形成4R-1HOG; 和（3）在D-氨基酸存在下使4R-1HOG与D-氨基转移酶接触以形成2R，4R-莫纳甜; 等等。 在（3）中，优选使用不具有或低能力从吲哚-3-丙酮酸形成D-色氨酸的D-氨基转移酶。

    Xylitol dehydrogenase of acetic acid bacteria and gene thereof
    75.
    发明授权
    Xylitol dehydrogenase of acetic acid bacteria and gene thereof 失效
    乙酸细菌的木糖醇脱氢酶及其基因

    公开(公告)号：US06242228B1

    公开(公告)日：2001-06-05

    申请号：US09363189

    申请日：1999-07-29

    申请人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

    发明人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P718

    CPC分类号： C12N9/0006 ,  C12P7/18

    摘要： Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

    摘要翻译： 通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA（其是以下（A）或（B）的蛋白质）作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇：（A） 具有SEQ ID NO：4的氨基酸序列;（B）具有SEQ ID NO：4的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或倒位，并且具有木糖醇 脱氢酶活性。