公开(公告)号：US20220325336A1

公开(公告)日：2022-10-13

申请号：US17641059

申请日：2020-09-07

申请人： bluebird bio, Inc.

发明人： Ilya Shestopalov

IPC分类号： C12Q1/6851 ,  C12Q1/70 ,  C12N5/0783 ,  C12N15/62 ,  C12N15/86

摘要： Disclosed herein are lentiviral vector transduction efficiency assays for gene therapy treatments. Also disclosed herein are methods for measuring transduction efficiency of a lentiviral vector.

公开(公告)号：US20220274117A1

公开(公告)日：2022-09-01

申请号：US17634799

申请日：2020-08-13

申请人： GIRM BIOSAFETY TECHNOLOGY CO. LTD.

发明人： Rong ZHOU ,  Wenkuan LIU ,  Xiao LI ,  Xianhua WANG ,  Hui XU ,  Zhichao ZHOU ,  Wenjuan GAO ,  Lei LI ,  Xiaohong LIAO

IPC分类号： B01L7/00 ,  C12Q1/6851

摘要： The present invention discloses an ultra-rapid PCR detection system, which comprises a temperature control device, a transmission device and a reaction tube fixing device; the temperature control device at least comprises two temperature control modules, wherein at least one of the temperature control modules is a high-temperature module and at least one of the temperature control modules is a low-temperature module; a heating temperature of the high-temperature module is a first preset temperature, and a heating temperature of the low-temperature module is a second preset temperature; the transmission device is in cooperation with the temperature control device and the reaction tube fixing device, so that a reaction tube on the reaction tube fixing device is switched between the high-temperature module and the low-temperature module; the device has a compact structure and the temperature setting of the high-temperature module and the low-temperature module can promote the rising and falling rate of the temperature of the reaction mixture in the reaction tube, in addition, the rapid fluorescence PCR detection system of the present invention has good repeatability of detection results and accurate results.

公开(公告)号：US20220267832A1

公开(公告)日：2022-08-25

申请号：US17735041

申请日：2022-05-02

申请人： Michael Edward Hogan ,  Benjamin Alan Katchman ,  Candy Mavis Rivas ,  Yasmine Eve Baca

发明人： Michael Edward Hogan ,  Benjamin Alan Katchman ,  Candy Mavis Rivas ,  Yasmine Eve Baca

IPC分类号： C12Q1/6806 ,  C12Q1/6851

摘要： Provided herein are methods for isolating nucleic acids from intact cells in a sample of intact cells and cell free nucleic acids. The intact cells and cell free nucleic acids are captured and concentrated within the pores of a silicate matrix in a microporous silicate filter. Within the silicate matrix the cell free nucleic acids are degraded with a nuclease, the intact cells are lysed with the released DNA binding to the silicate, the nuclease treated cell free nucleic acids and contaminants from the lysed are washed from the silicate matrix and the DNA bound to the silicate is eluted therefrom to form an isolated DNA product.

公开(公告)号：US20220267828A1

公开(公告)日：2022-08-25

申请号：US17548037

申请日：2021-12-10

申请人： Industrial Technology Research Institute

发明人： Min-Yuan CHOU ,  Kuang-Chi CHENG ,  Ming-Hua YANG ,  Jiun-Lin GUO

IPC分类号： C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6876

摘要： A GAPDH nucleic acid detection kit includes a primer set for detecting GAPDH nucleic acid. The primer set for detecting GAPDH nucleic acid includes a forward inner primer for GAPDH nucleic acids, a forward outer primer for GAPDH nucleic acids, a backward inner primer for GAPDH nucleic acids and a backward outer primer for GAPDH nucleic acids. The primer set for detecting GAPDH nucleic acid is used in a loop-mediated isothermal amplification (LAMP).

公开(公告)号：US11414698B2

公开(公告)日：2022-08-16

申请号：US16357706

申请日：2019-03-19

申请人： CHANG GUNG MEDICAL FOUNDATION CHANG GUNG MEMORIAL HOSPITAL AT CHIAYI

发明人： Chih-Cheng Chen ,  Chia-Chen Hsu

IPC分类号： C12Q1/68 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6827 ,  C12Q1/6876

摘要： Disclosed herein is a method of quantifying a mutant allele burden of a target gene in a subject. The method includes providing a first plasmid that includes a mutant allele sequence and an internal control sequence, and a second plasmid that includes a wild-type allele sequence and the internal control sequence, and subjecting DNA of the subject to quantitative polymerase chain reaction to measure a mutant allele expression level of the target gene, so as to determine the mutant allele burden of the target gene in the subject based on a standard curve of the mutant allele burden of the target gene created by serial dilution of the first and second plasmids.

公开(公告)号：US20220220535A1

公开(公告)日：2022-07-14

申请号：US17147549

申请日：2021-01-13

申请人： BENED BIOMEDICAL CO., LTD.

发明人： Ying-Chieh TSAI ,  Chien-Chen WU ,  Chih-Chieh HSU

IPC分类号： C12Q1/689 ,  C12Q1/6851

摘要： Provided are compositions and methods for identifying a specific strain of Lactobacillus plantarum, e.g., Lactobacillus plantarum subsp. plantarum PS128 deposited under DSMZ Accession No. DSM 28632.

公开(公告)号：US20220213535A1

公开(公告)日：2022-07-07

申请号：US17698230

申请日：2022-03-18

申请人： SEEGENE, INC.

发明人： Jong Yoon CHUN ,  Young Jo LEE

IPC分类号： C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6818 ,  C12Q1/6858

摘要： The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adoptable for detection of the presence of the target nucleic acid sequence.

公开(公告)号：US11377695B2

公开(公告)日：2022-07-05

申请号：US16102564

申请日：2018-08-13

申请人： CHRONIX BIOMEDICAL

发明人： Ekkehard Schuetz ,  Julia Beck ,  Howard Urnovitz

IPC分类号： C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6883

摘要： The invention provides methods and reagents for diagnosing breast cancer that are based on the detection of biomarkers in the circulating nucleic acids from a patient to be evaluated.

公开(公告)号：US11377691B2

公开(公告)日：2022-07-05

申请号：US16196092

申请日：2018-11-20

申请人： Chang Gung Memorial Hospital, Kaohsiung

发明人： Ying-Hsien Huang ,  Ho-Chang Kuo

IPC分类号： C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6816 ,  C12Q1/6851 ,  A61P9/00 ,  A61K39/00

摘要： The present invention relates to methods that use novel biomarkers for diagnosing and/or treating Kawasaki diseases. The novel biomarkers of the invention show altered cytosine methylations state of certain CpG loci in subject with Kawasaki Disease relative to subject without Kawasaki diseases. Also provided are kits comprising at least one primer or reagent specific for the altered cytosine methylation state of certain CpG loci to detect Kawasaki Disease.

公开(公告)号：US20220205025A1

公开(公告)日：2022-06-30

申请号：US17611504

申请日：2020-05-12

申请人： Biotype GmbH

发明人： Karim Tabiti

IPC分类号： C12Q1/6827 ,  C12Q1/6851

摘要： The present invention is directed to a method for determining of at least one microsatellite instability (MSI) based on a shift in a capillary electrophoresis (CE) profile (CE profile shift), the CE profile shift being determined by a comparison between the capillary electrophoresis (CE) profile of a target sequence of at least one microsatellite (MSI target profile) and the capillary electrophoresis (CE) profile of its specific wild type sequence (MS wild type profile). Further, the invention encompass suitable primer for use in said method, a kit comprising all essential components for performing said method successfully, a complete closed device as a system, namely “MSI Modaplex Analysis System” and a method for diagnosis of MSI phenotypes associated with an inflammation, cancer, inflammation associated cancer and/or auto immune disease, wherein the diagnosis comprises the method for determining of at least one CE profile shift as mentioned above. Finally, the present invention is directed to the use of an improved MSI panel for the determination and preferably diagnosis of MSI tumors, said panel consist of the STR biomarker NR-21, NR-24, Mono27, D2S123, D5S346, D17S250, Bat-25 and Bat-26.