公开(公告)号：US07985570B2

公开(公告)日：2011-07-26

申请号：US12718086

申请日：2010-03-05

申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

IPC分类号： C12N9/48 ,  C11D3/386

CPC分类号： C12N9/52 ,  C11D3/38609 ,  C11D3/38618

摘要： The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

摘要翻译： 本申请涉及两个彼此相似的新型蛋白酶（SEQ ID NO：4和7）。 两种蛋白酶的DNA从土壤样品获得，并且C末端缺失，产生其蛋白水解活性片段（SEQ ID NO：5和8）。 本发明还提供了类似于SEQ ID NO：4至少90％或SEQ ID NO：7至87.5％的所有碱性蛋白酶，以及可归纳于SEQ ID NO：7衍生的共有序列（SEQ ID NO：9） 此外，它涉及与相关核酸（SEQ ID NO：3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。