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1.发明申请NOVEL ALKALINE PROTEASES AND DETERGENTS AND CLEANERS COMPRISING THESE NOVEL ALKALINE PROTEASES 有权新型碱性蛋白质和包含这些新碱性蛋白质的洗涤剂和清洁剂公开(公告)号：US20070212706A1
公开(公告)日：2007-09-13
申请号：US11552061
申请日：2006-10-23
CPC分类号： C12N9/52 , C11D3/38609 , C11D3/38618
摘要： The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and C-terminally deleted, likewise proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.
摘要翻译： 本申请涉及两个彼此相似的碱性蛋白酶（SEQ ID NO.4和7），其DNA是从土壤样品获得的，并且C末端缺失，同样蛋白水解活性片段（SEQ ID NO.5 和8），所有碱性蛋白酶类似于至少90％的SEQ ID NO。 4或至87.5％。 7，以及可以在源自SEQ ID NO：1的共有序列（SEQ ID NO.9）中总结的那些。 此外，它涉及与相关核酸（SEQ ID NO.3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。
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2.发明申请Novel alkaline protease and washing and cleaning products containing said novel alkaline protease 有权新型碱性蛋白酶和含有所述新型碱性蛋白酶的洗涤和清洁产品公开(公告)号：US20070010417A1
公开(公告)日：2007-01-11
申请号：US11473708
申请日：2006-06-22
摘要： The invention relates to a novel alkaline protease, the DNA thereof having been taken from a bottom sample, to all alkaline proteases that are at least 40% identical, and to all nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease, comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and to all alkaline proteases that are at least 60% identical, or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases and especially describes the use thereof in washing and cleaning products.
摘要翻译： 本发明涉及一种新的碱性蛋白酶，其DNA从底部样品中获取至所有与至少40％相同的碱性蛋白酶，以及所有与相关核酸具有至少50％同源性的核酸 。 本发明还涉及所述蛋白酶的片段，其除了编码该基因片段的氨基酸位置108至325之外，还涉及至少60％相同的所有碱性蛋白酶，或至少70个核酸 ％相同。 此外，本发明定义了用于所述蛋白酶的技术可能性，并且特别描述了在洗涤和清洁产品中的用途。
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3.发明授权Alkaline proteases and detergents and cleaners comprising these alkaline proteases 有权包含这些碱性蛋白酶的碱性蛋白酶和洗涤剂和清洁剂公开(公告)号：US07985570B2
公开(公告)日：2011-07-26
申请号：US12718086
申请日：2010-03-05
CPC分类号： C12N9/52 , C11D3/38609 , C11D3/38618
摘要： The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.
摘要翻译： 本申请涉及两个彼此相似的新型蛋白酶（SEQ ID NO：4和7）。 两种蛋白酶的DNA从土壤样品获得，并且C末端缺失，产生其蛋白水解活性片段（SEQ ID NO：5和8）。 本发明还提供了类似于SEQ ID NO：4至少90％或SEQ ID NO：7至87.5％的所有碱性蛋白酶，以及可归纳于SEQ ID NO：7衍生的共有序列（SEQ ID NO：9） 此外，它涉及与相关核酸（SEQ ID NO：3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。
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4.发明申请Alkaline Proteases and Detergents and Cleaners Comprising These Alkaline Proteases 有权碱性蛋白酶和包含这些碱性蛋白酶的洗涤剂和清洁剂公开(公告)号：US20100298198A1
公开(公告)日：2010-11-25
申请号：US12718086
申请日：2010-03-05
CPC分类号： C12N9/52 , C11D3/38609 , C11D3/38618
摘要： The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and C-terminally deleted, likewise proteolytically active fragments thereof (SEQ ID NO: 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.
摘要翻译： 本申请涉及两个彼此相似的新型蛋白酶（SEQ ID NO：4和7），其DNA从土壤样品获得，并且C末端缺失，同样蛋白水解活性片段（SEQ ID NO：5和 8），所有与SEQ ID NO：4至少90％相同的碱性蛋白酶或与SEQ ID NO：7相似至87.5％的那些，以及源自SEQ ID NO：7的共有序列（SEQ ID NO：9） 此外，它涉及与相关核酸（SEQ ID NO：3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。
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5.发明授权Alkaline protease and washing and cleaning products containing said novel alkaline protease 有权碱性蛋白酶和含有所述新型碱性蛋白酶的洗涤和清洁产品公开(公告)号：US07811076B2
公开(公告)日：2010-10-12
申请号：US11473708
申请日：2006-06-22
IPC分类号： C12N9/48
摘要： An alkaline protease, the DNA thereof having been taken from a bottom sample, alkaline proteases that are at least 40% identical, and nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and alkaline proteases that are at least 60% identical or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases, especially use thereof in washing and cleaning products.
摘要翻译： 碱性蛋白酶，其从底部样品取得的DNA，至少40％相同的碱性蛋白酶以及与相关核酸具有至少50％同一性的核酸。 除了编码该基因片段的基因片段以外，本发明还涉及包含氨基酸位置108至325的所述蛋白酶片段，以及至少60％相同的碱性蛋白酶或至少70％相同的核酸。 此外，本发明定义了用于所述蛋白酶的技术可能性，特别是在洗涤和清洁产品中的应用。
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6.发明授权Alkaline proteases and detergents and cleaners comprising these alkaline proteases 有权包含这些碱性蛋白酶的碱性蛋白酶和洗涤剂和清洁剂公开(公告)号：US07691618B2
公开(公告)日：2010-04-06
申请号：US11552061
申请日：2006-10-23
IPC分类号： C12N9/48
CPC分类号： C12N9/52 , C11D3/38609 , C11D3/38618
摘要： The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and which were C-terminally deleted. The present application also provides proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore, the present application relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, the present application relates to use for these proteases and especially describes their use in detergents and cleaners.
摘要翻译： 本申请涉及两种彼此相似的新型碱性蛋白酶（SEQ ID NO.4和7），其DNA从土壤样品中获得，并且被C末端缺失。 本申请还提供了其蛋白水解活性片段（SEQ ID NO.5和8），所有碱性蛋白酶类似于SEQ ID NO.1至少90％。 4或至87.5％。 7，以及可以在源自SEQ ID NO：1的共有序列（SEQ ID NO.9）中总结的那些。 此外，本申请涉及与相关核酸（SEQ ID NO.3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，本申请涉及这些蛋白酶的用途，特别描述了它们在洗涤剂和清洁剂中的用途。
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公开(公告)号：US20050003419A1
公开(公告)日：2005-01-06
申请号：US10872874
申请日：2004-06-21
申请人： Roland Breves , Karl-Heinz Maurer , Jurgen Eck , Patrick Lorenz , Holger Zinke
发明人： Roland Breves , Karl-Heinz Maurer , Jurgen Eck , Patrick Lorenz , Holger Zinke
IPC分类号： D06L1/20 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/09 , C12N15/56 , C12P7/06 , C12P19/14 , C12Q1/68 , C12S11/00 , D06L4/40 , C07H21/04 , C12N9/20
CPC分类号： C12N9/2414 , C07K2319/00 , C11D3/386 , Y02E50/17
摘要： The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.
摘要翻译： 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸，基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品，以及与其对应的方法和可能的用途是特别有趣的。
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公开(公告)号：US07300782B2
公开(公告)日：2007-11-27
申请号：US10872874
申请日：2004-06-21
申请人： Roland Breves , Karl-Heinz Maurer , Jürgen Eck , Patrick Lorenz , Holger Zinke
发明人： Roland Breves , Karl-Heinz Maurer , Jürgen Eck , Patrick Lorenz , Holger Zinke
CPC分类号： C12N9/2414 , C07K2319/00 , C11D3/386 , Y02E50/17
摘要： The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.
摘要翻译： 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸，基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品，以及与其对应的方法和可能的用途是特别有趣的。
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公开(公告)号：US09045713B2
公开(公告)日：2015-06-02
申请号：US13502945
申请日：2010-10-27
申请人： Khanh Quoc Nguyen , Kornelia Titze , Tatiana Schwarz , Silvia Paladino , Volker Marschner , Patrick Lorenz
发明人： Khanh Quoc Nguyen , Kornelia Titze , Tatiana Schwarz , Silvia Paladino , Volker Marschner , Patrick Lorenz
IPC分类号： C12N9/00 , C12N9/20 , C12N1/20 , D06M16/00 , C12N15/00 , C11B3/00 , A23J7/00 , A23K1/165 , C12N9/16
CPC分类号： C12N9/18 , A23D9/00 , A23D9/02 , A23D9/04 , A23J7/00 , A23K20/189 , A23V2002/00 , C11B3/00 , C11B3/003 , C12N9/16 , C12Y301/01004 , C12Y301/01005 , C12Y301/01032
摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).
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公开(公告)号：US07700311B2
公开(公告)日：2010-04-20
申请号：US10593119
申请日：2005-03-10
申请人： Renate Schulze , Patrick Lorenz , Jürgen Eck , Oliver May , Harald Gröger , Harald Trauthwein
发明人： Renate Schulze , Patrick Lorenz , Jürgen Eck , Oliver May , Harald Gröger , Harald Trauthwein
CPC分类号： C12P7/02 , C12N9/0006 , C12Y101/01002
摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to nonhuman hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohols which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohols with the formation of aldehydes or ketones.
摘要翻译： 本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。 本发明还涉及编码所述多肽的核酸，非人宿主细胞和宿主细胞以及可用于制备所需产物的反应体系。 本发明的多肽优选用于从醛或酮开始制备可用作药物中间体的伯和对映体纯的仲醇。 或者，本发明的多肽也可以用于逆反应，即醇形成醛或酮的氧化。
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