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公开(公告)号：US06916656B2
公开(公告)日：2005-07-12
申请号：US10487235
申请日：2002-04-05
CPC分类号： C12M35/02 , A61K2039/5152 , A61K2039/5154 , C12N5/16 , C12N13/00 , H01L2224/83856 , H01L2924/01019
摘要： An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior to being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linear algorithms.
摘要翻译： 本发明的一个目的是提供一种在使生物细胞经历细胞融合脉冲之前处理生物细胞的方法，其包括用以非线性方式改变幅度的融合前电场波形来处理生物细胞的步骤， 相对于时间，使得生物细胞首先用相对低幅度，长持续时间的预融合电场波形对准，然后用相对较高幅度，短持续时间的预融合电场波形压缩，导致增加的细胞膜接触先 进行细胞融合。 非线性预融合电场波形可以以连续的方式以S形方式以相邻步骤中逐步增加的波形以逐步增加的波形在非相邻步骤中逐步增加，并以 按照非线性算法。
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公开(公告)号：US6078490A
公开(公告)日：2000-06-20
申请号：US313260
申请日：1999-05-18
申请人： Richard E. Walters
发明人： Richard E. Walters
摘要： A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.
摘要翻译： 提供了一种利用脉冲电场处理材料，特别是有机材料的方法，其中该方法包括将具有至少三个脉冲的敏捷脉冲序列应用于材料的步骤，其中敏捷脉冲序列具有一个，两个或三个 具有以下特征：（1）所述至少三个脉冲中的至少两个脉冲在脉冲幅度上彼此不同; （2）至少三个脉冲中的至少两个脉冲在脉冲宽度上彼此不同; 和（3）至少三个脉冲中的第二组的第一组的第一脉冲间隔对于所述至少三个脉冲中的两个的第二组的第二脉冲间隔是不同的。 当在电穿孔方法中处理生物细胞以形成孔时，诱导的孔被维持相当长的时间段，并且维持生物细胞的存活力。
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公开(公告)号：US6010613A
公开(公告)日：2000-01-04
申请号：US569664
申请日：1995-12-08
申请人： Richard E. Walters , Alan D. King
发明人： Richard E. Walters , Alan D. King
IPC分类号： C12N13/00
摘要： A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.
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4.发明授权Apparatus for receiving and aligning a multiple electrode pair array and a multiple treatment cell for use in electroporation 有权用于接收和对准多个电极对阵列和用于电穿孔的多重处理电池的装置公开(公告)号：US06878538B1
公开(公告)日：2005-04-12
申请号：US10694344
申请日：2003-10-28
IPC分类号： B01L9/00 , C12M1/42 , C12M3/00 , C12N20060101
CPC分类号： B01L9/523 , B01L2300/046 , B01L2300/0829 , C12M23/12 , C12M23/48 , C12M35/02 , Y10S435/809
摘要： An apparatus is provided for receiving and aligning a multiple electrode pair array and a multiple well plate used in electroporation. The apparatus includes a housing which includes a bottom housing portion and a pair of side housing portions which project upward from the bottom housing portion. A pair of board-reception structures are adjacent to the inner portions of the side housing portions. Multiple well plate engaging and disengaging means, supported by the housing, are provided for engaging a multiple well plate with a multiple electrode pair array and for disengaging a multiple well plate from a multiple electrode pair array. Preferably, the multiple well plate engaging and disengaging means include a multiple well plate lifting and lowering assembly. The multiple well plate lifting and lowering assembly can include lifting/lowering handles located outside the housing.
摘要翻译： 提供了用于接收和对准多电极对阵列和用于电穿孔的多孔板的装置。 该装置包括壳体，该壳体包括底部壳体部分和从底部壳体部分向上突出的一对侧部壳体部分。 一对板接收结构邻近侧壳体部分的内部部分。 提供由壳体支撑的多个孔板接合和分离装置，用于使多孔板与多个电极对阵列接合并且用于使多个孔板与多个电极对阵列分离。 优选地，多孔板接合和分离装置包括多孔板升降组件。 多孔板升降组件可以包括位于壳体外部的升降手柄。
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公开(公告)号：US07824901B2
公开(公告)日：2010-11-02
申请号：US10580429
申请日：2003-12-01
申请人： Richard E. Walters
发明人： Richard E. Walters
摘要： An apparatus is provided for carrying out fusion of biological cells (10) and includes a base member (24) on which a conductive outer electrode (18) is supported and has an outer electrode radius (r2) and has an electrode height (19). A conductive inner electrode (20) is supported on the base member (24) and has an inner electrode radius (r1) and also has the electrode height (19). The outer and inner electrodes (18,20) are spaced apart from each other by a gap which defines a fusion chamber (14). The inner electrode radius (r1), the outer electrode radius (r2), and the gap are selected in accordance with a predetermined range of selectable ratios (r1/r2) in a range from 0.7 to 0.9, wherein a selected gap is limited by the range of selectable ratios (r1/r2), and wherein a determined ratio (r1/r2) among the selectable ratios is based on the selected gap, such that compression between the biological cells (10) and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in the fusion chamber (14).
摘要翻译： 提供一种用于进行生物电池（10）熔化的装置，并且包括一个支撑导电外电极（18）并具有外电极半径（r2）并且具有电极高度（19）的基体部件（24） 。 导电内电极（20）支撑在基体部件（24）上，内电极半径（r1）也具有电极高度（19）。 外部和内部电极（18,20）通过限定熔化室（14）的间隙彼此间隔开。 内部电极半径（r1），外部电极半径（r2）和间隙根据可选择比率（r1 / r2）的预定范围在0.7至0.9的范围内选择，其中所选择的间隙由 可选择比率（r1 / r2）的范围，并且其中可选择比率之间的确定比率（r1 / r2）基于所选择的间隙，使得生物细胞（10）之间的压缩和细胞膜之间的渗透性最大化， 使熔化室（14）中的细胞融合最小化温度上升。
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6.发明申请METHOD AND APPARATUS FOR TREATING MATERIALS WITH ELECTRICAL FIELDS HAVING VARYING ORIENTATIONS 失效用于处理具有变化方位的电场的材料的方法和装置公开(公告)号：US20030159943A2
公开(公告)日：2003-08-28
申请号：US10265133
申请日：2002-10-07
IPC分类号： C25C003/16
摘要： Abstract of DisclosureThe object of the invention is to provide a method and apparatus for treating membrane-containing material with electrical fields, in vitro, and with an added treating substance. With the method, a plurality of electrodes are arrayed around the material to be treated and are connected to outputs of an electrode selection apparatus. Inputs of the electrode selection apparatus are connected to outputs of a pulse sequence generator. A treating substance is added to the membrane-containing material. Electrical pulses are applied to the electrode selection apparatus and are routed through the electrode selection apparatus in a predetermined, computer-controlled sequence to selected electrodes in the array of electrodes, whereby the membrane-containing material is treated with the added treating substance and with electrical fields. The routing of applied pulses through the electrode selection apparatus to selected electrodes can be done in an enormous number of ways.
摘要翻译： 发明内容本发明的目的是提供一种用于在体外用添加的处理物质用电场处理含膜材料的方法和装置。 利用该方法，将多个电极围绕待处理的材料排列并连接到电极选择装置的输出端。 电极选择装置的输入连接到脉冲序列发生器的输出端。 将处理物质加入到含膜材料中。 将电脉冲施加到电极选择装置，并以预定的计算机控制的顺序通过电极选择装置引导到电极阵列中的选定电极，由此将含膜材料用添加的处理物质和电 领域。 施加的脉冲通过电极选择装置到选定电极的路由可以以很多方式进行。
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