公开(公告)号：US20240351928A1

公开(公告)日：2024-10-24

申请号：US18637984

申请日：2024-04-17

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： Julian John Alexander VAN DER MADE ,  Kartik CHANDRAN ,  Elizabetth Amy LANDIS ,  Ruby LAI

IPC: C02F3/34 ,  C02F1/467 ,  C02F1/72 ,  C02F101/34 ,  C02F101/36 ,  C02F103/34 ,  C12M1/00 ,  C12M1/06 ,  C12M1/12 ,  C12M1/42 ,  C12N1/14

CPC classification number: C02F3/347 ,  C02F1/4672 ,  C02F1/722 ,  C12M25/02 ,  C12M27/06 ,  C12M29/06 ,  C12M35/02 ,  C12N1/14 ,  C02F2101/34 ,  C02F2101/36 ,  C02F2103/34 ,  C02F2305/026

Abstract: A fungal culture is provided that incorporates concentrations of Fenton reactants and white rot fungi biomass. These components synergize to degrade recalcitrant organic compounds such as lignins, perfluorochemicals including PFOA, plastics, and related compounds, particularly with 1.5 mM H2O2, 1 mM Fe2+, and Phanerochaete chrysosporium exhibiting lignin degrading capabilities comparable to cultures of Fenton-only reaction mediums with significantly higher concentrations of hydrogen peroxide. Both the fungi and the Fenton reactants work to degrade the organic compounds. Additionally, the Fenton reactants impose oxidative stress on fast-growing microbial competitors such as E. coli, selectively inhibiting the competing bacteria and their disadvantageous effects on white rot fungi activity. The white rot fungi recycle Fe(II) ions to reduce Fenton reactant input burden. An electro-Fenton reaction process can further reduce this burden by replenishing H2O2. The resulting systems and methods demonstrate more economical and sustainable treatment of recalcitrant organic compounds.

公开(公告)号：US20240327774A1

公开(公告)日：2024-10-03

申请号：US18128441

申请日：2023-03-30

Applicant: ORIENTAL SYSTEM TECHNOLOGY INC.

Inventor： Wen-Chie HUANG ,  Chein-Hsun WANG ,  Y. H. CHEN ,  Chin-Hsiang CHANG ,  Jenping KU ,  Yu-Chih LIANG

IPC: C12M1/42 ,  B01L3/00 ,  C12M3/06

CPC classification number: C12M35/02 ,  B01L3/502761 ,  C12M23/16 ,  B01L2200/0663 ,  B01L2300/0681 ,  B01L2400/0439 ,  B01L2400/06

Abstract: A microfluidic electroporation device for exogenous molecules transfection is disclosed. The microfluidic electroporation device includes an electroporation chamber assembly, an ultrasound vibrator, and a controller. The electroporation chamber assembly includes an input chamber for exogenous molecules, a MEMS filter, an activation chamber and a MEMS plate. The MEMS plate holds cells within individual cavity for electroporation. Both the MEMS filter and the MEMS plate are made of semiconductor process by wet etching and/or ICP dry etching with V-shaped cavities. The top surfaces of the MEMS filter and the MEMS plate are coated with metal layer for applying electric field during the electroporation process. The electroporation chamber assembly is attached to an ultrasound vibrator which is operated intermittently to allow cells to be fixed in the cavity of the MEMS plate during electroporation process and popped out for collection after electroporation process.

公开(公告)号：US20240301341A1

公开(公告)日：2024-09-12

申请号：US18606828

申请日：2024-03-15

Applicant: JIAN CHEN

Inventor： JIAN CHEN

IPC: C12M1/42 ,  C12M1/32 ,  C12N13/00 ,  C12N15/82 ,  C12N15/87 ,  G01N33/487

CPC classification number: C12M35/02 ,  C12M23/12 ,  C12N13/00 ,  C12N15/8206 ,  C12N15/8207 ,  C12N15/87 ,  G01N33/48728

Abstract: An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.

公开(公告)号：US12006507B2

公开(公告)日：2024-06-11

申请号：US16935987

申请日：2020-07-22

Applicant: NantCell, Inc.

Inventor： Chih-Wei Chang ,  Jon Thomas Van Lew ,  Artin Mehrabi

IPC: C12M3/00 ,  A61N1/08 ,  C12M1/42 ,  C12N15/00 ,  C12N15/87 ,  A61N1/04 ,  A61N1/378 ,  H03K3/00

CPC classification number: C12N15/87 ,  A61N1/08 ,  C12M35/02 ,  A61N1/0476 ,  A61N1/378 ,  H03K3/00

Abstract: An apparatus for electroporating cells with a cargo includes electrodes defining a path for a fluid including the cells and the cargo to flow, a power source coupled across the electrodes, and a control circuit. In some examples, the control circuit is configured to detect a decrease in an induced current due to an increase in a resistance between the electrodes, and control the power source to increase the induced current to maintain an electric field between the electrodes. A future value of the resistance between the electrodes may be predicted based on previous values of the resistance. In other examples, the control circuit is configured to detect parameters of the fluid flowing between the electrodes, and control the power source to generate or stop generating electrical pulses in response to detecting the parameters. Other example apparatuses, and methods of electroporating cells with a cargo is also disclosed.

公开(公告)号：US20240182838A1

公开(公告)日：2024-06-06

申请号：US18524718

申请日：2023-11-30

Applicant: Horia Hulubei National Institute for R & D in Physics and Nuclear Engineering (IFIN-HH)

Inventor： Klaus SPOHR

IPC: C12M1/42 ,  C12M1/00 ,  C12N13/00

CPC classification number: C12M35/04 ,  C12M27/20 ,  C12M35/02 ,  C12N13/00

Abstract: A method for porating cells for loading with a substance, preferably nuclides for radiotherapy, performs porating in a porating device with cells to be porated in a liquid with the substance. The cell membranes are permeabilized by sonoporation or laser poration, then the cells are incubated in a porating device incubation section. Moreover, a device for porating cells, in particular by sonoporation or laser porating, and for loading cells, particularly immunocompetent cells, in particular γδ T cells, includes inlet and outlet openings for a cell solution with cells to be porated and a substance to be taken up by the cells as a load and preferably microbubbles. A poration arrangement extending between the openings has a collector at the inlet opening for collecting cell solution and a reactor in flow connection with the collector. Inside the reactor the cell membranes may be porated and the cells may uptake the load.

公开(公告)号：US20240133873A1

公开(公告)日：2024-04-25

申请号：US18537653

申请日：2023-12-12

Applicant: Milica Radisic

Inventor： Jason Miklas ,  Milica Radisic ,  Nimalan Thavandiran ,  Sara Vasconcelos ,  Yun Xiao ,  Boyang Zhang ,  Yimu Zhao

IPC: G01N33/50 ,  C12M1/12 ,  C12M1/32 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00 ,  C12M3/06

CPC classification number: G01N33/5082 ,  C12M21/08 ,  C12M23/12 ,  C12M23/16 ,  C12M25/14 ,  C12M35/02 ,  C12M41/46 ,  G01N33/502 ,  G01N33/5061

Abstract: The present disclosure provides methods, compositions, and devices for making and using three-dimensional biological tissues that accurately mimic native physiology, architecture, and other properties of native tissues for use in, among other applications, drug testing, tissue repair and/or treatment, and regenerative medicine.

公开(公告)号：US11965149B2

公开(公告)日：2024-04-23

申请号：US16586989

申请日：2019-09-29

Applicant: Jian Chen

Inventor： Jian Chen

IPC: C12M1/42 ,  B01L3/00 ,  C12M1/12 ,  C12M1/24

CPC classification number: C12M35/02 ,  B01L3/508 ,  C12M1/24 ,  C12M1/42 ,  C12M23/06 ,  C12M23/08 ,  B01L2300/027 ,  B01L2300/0663

Abstract: The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed.

公开(公告)号：US11952565B2

公开(公告)日：2024-04-09

申请号：US17147662

申请日：2021-01-13

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Don-Wook Lee ,  Young Jun Hong

IPC: C12M1/42

CPC classification number: C12M35/02

Abstract: An electrical stimulation device includes a terminal electrode having an opening at a center thereof, a ground electrode having an area smaller than that of the terminal electrode, and a power source connected to the terminal electrode and the ground electrode and configured to apply an electrical stimulus to an object disposed between the terminal electrode and the ground electrode by applying a terminal voltage to the terminal electrode.

公开(公告)号：US11920247B2

公开(公告)日：2024-03-05

申请号：US17481545

申请日：2021-09-22

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Martin Van Den Berghe ,  Kenneth H. Nealson ,  A. Joshua West

IPC: C25B1/00 ,  C12M1/00 ,  C12M1/107 ,  C12M1/42 ,  C25B1/04 ,  C25B1/50 ,  C25B3/25 ,  C25B9/19

CPC classification number: C25B1/50 ,  C12M21/04 ,  C12M23/34 ,  C12M35/02 ,  C25B1/04 ,  C25B3/25 ,  C25B9/19

Abstract: In various embodiments of the present disclosure, an oxygen-generating bioelectrical reactor comprises two chambers, a first oxidative chamber configured for the abiotic oxidation of water to generate molecular oxygen, and a second reductive chamber configured for the biotic reduction of an insoluble metal(loid) oxide or hydroxide. In various embodiments, the biotic reduction comprises microbially-catalyzed metal(loid) ion reduction of the insoluble metal(loid) oxide or hydroxide, wherein a dissimilatory metal(loid) reducing microorganism transfers electrons obtained from the oxidation of the water extracellularly to the metal(loid) ions.

公开(公告)号：US11913940B2

公开(公告)日：2024-02-27

申请号：US16259399

申请日：2019-01-28

Applicant: Milica Radisic

Inventor： Jason Miklas ,  Milica Radisic ,  Nimalan Thavandiran ,  Sara Vasconcelos ,  Yun Xiao ,  Boyang Zhang ,  Yimu Zhao

IPC: G01N33/50 ,  C12M3/00 ,  C12M1/32 ,  C12M3/06 ,  C12M1/12 ,  C12M1/42 ,  C12M1/34

CPC classification number: G01N33/5082 ,  C12M21/08 ,  C12M23/12 ,  C12M23/16 ,  C12M25/14 ,  C12M35/02 ,  C12M41/46 ,  G01N33/502 ,  G01N33/5061

Abstract: The present disclosure provides methods, compositions, and devices for making and using three-dimensional biological tissues that accurately mimic native physiology, architecture, and other properties of native tissues for use in, among other applications, drug testing, tissue repair and/or treatment, and regenerative medicine.