公开(公告)号：US09034611B2

公开(公告)日：2015-05-19

申请号：US13823696

申请日：2011-10-24

申请人： Wanda Dischert ,  Perrine Vasseur ,  Cédric Boisart ,  Rainer Figge

发明人： Wanda Dischert ,  Perrine Vasseur ,  Cédric Boisart ,  Rainer Figge

IPC分类号： C12P13/12 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N9/02 ,  C12N15/52

CPC分类号： C12P13/12 ,  C12N9/0036 ,  C12N15/52

摘要： The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.

摘要翻译： 本发明涉及用于生产甲硫氨酸的微生物，其中所述微生物被修饰以增强PntAB的转氢酶活性。 在本发明的优选方面，所述微生物中转氢酶UdhA的活性减弱。 本发明还涉及通过发酵生产甲硫氨酸的方法。

公开(公告)号：US20100285547A1

公开(公告)日：2010-11-11

申请号：US12532405

申请日：2008-03-21

申请人： Philippe Soucaille ,  Francois Voelker ,  Rainer Figge

发明人： Philippe Soucaille ,  Francois Voelker ,  Rainer Figge

IPC分类号： C12P7/18 ,  C12N15/01

CPC分类号： C12P7/18 ,  C12N9/90 ,  C12N15/01 ,  C40B10/00

摘要： The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source.The said method comprises: growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of the tpiA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain, then selecting and isolating the evolved strain having an increased 1,2 propanediol production rate, then reconstructing a functional tpiA gene in the evolved strain; The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a carbon source into 1,2-propanediol without by-products and with the best possible yield.

摘要翻译： 本发明涉及一种组合进化和合理设计的新方法，用于制备用于从碳源生产1,2-丙二醇的微生物菌株。 所述方法包括：在合适的生长培养基中在选择压力下生长初始菌株，所述初始细菌菌株包括tpiA基因表达的减弱和减少参与将甲基乙二醛转化为乳酸的至少一个基因的表达 为了促进所述初始菌株的进化，然后选择和分离具有增加的1,2-丙二醇生产速率的进化菌株，然后在演化菌株中重建功能性tpiA基因; 本发明还涉及如所获得的进化菌株，其可以进一步经遗传修饰以优化碳源转化为1,2-丙二醇而不产生副产物并以最好的产率。

公开(公告)号：US20100261239A1

公开(公告)日：2010-10-14

申请号：US12532423

申请日：2008-03-21

申请人： Philippe Soucaille ,  Francois Voelker ,  Rainer Figge

发明人： Philippe Soucaille ,  Francois Voelker ,  Rainer Figge

IPC分类号： C12P7/18 ,  C12N1/21

CPC分类号： C12P7/18 ,  C12N9/0008

摘要： Microorganism useful for the production of 1,2-propanediol from a carbon source, wherein said microorganism is characterized by: an improved activity of the biosynthesis pathway from dihydroxyacetone phosphate to 1,2-propanediol, and an attenuated activity of the glyceraldehyde 3-phosphate dehydrogenase The invention is also related to a method for producing 1,2-propanediol by fermentation with a microorganism according to the invention.

摘要翻译： 用于从碳源生产1,2-丙二醇的微生物，其中所述微生物的特征在于：从二羟丙基丙酮磷酸酯向1,2-丙二醇的生物合成途径的活性提高，甘油醛-3-磷酸酯的减毒活性 脱氢酶本发明还涉及通过用本发明的微生物发酵生产1,2-丙二醇的方法。