公开(公告)号：US20140322783A1

公开(公告)日：2014-10-30

申请号：US14361948

申请日：2012-11-30

申请人： Adam M. GUSS ,  Mascoma Corporation ,  Dartmouth College

发明人： Jonathan Lo ,  Adam M. Guss ,  Johannes P. Van Dijken ,  Arthur J. Shaw, IV ,  Daniel G. Olson ,  Christopher D. Herring

IPC分类号： C12P7/06 ,  C12N15/74

摘要： The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

摘要翻译： 本发明提供了对重组宿主细胞中辅因子使用的操作以增加所需产物的形成。 在一些实施方案中，本发明提供了包含一种或多种天然酶中的突变的重组微生物，使得它们的辅因子特异性以使得细胞中的总辅助因子在特定途径中平衡的方式改变，并且增加 细胞内的特定产物形成。 在一些实施方案中，内源性酶由具有来自不同物种的替代辅因子特异性的酶代替。