公开(公告)号：US20150024450A1

公开(公告)日：2015-01-22

申请号：US13888610

申请日：2013-05-07

申请人： Mascoma Corporation

发明人： David Anthony Hogsett ,  Vineet Badriphrajad Rajgarhia

IPC分类号： C12P7/10 ,  C12N15/74

CPC分类号： C12P7/10 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  C12P2203/00 ,  C12Y101/01002 ,  C12Y101/01027 ,  C12Y101/02003 ,  C12Y203/01008 ,  C12Y207/01017 ,  C12Y207/02001 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of ethanol. Another aspect of the invention relates to a process for converting lignocellulosic biomass to ethanol, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

摘要翻译： 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与 有机酸或其盐的代谢产生，从而增加所述嗜热或嗜温微生物产生乙醇作为发酵产物的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乙醇代谢产生的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乙醇的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。

公开(公告)号：US20140356921A1

公开(公告)日：2014-12-04

申请号：US14348231

申请日：2012-09-28

申请人： Dartmouth College ,  Mascoma Corporation

发明人： Yu Deng ,  Daniel G. Olson ,  Johannes Pieter van Dijken ,  Arthur J. Shaw, IV ,  Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Christopher D. Herring ,  Stephen R. Rogers ,  Frank Agbogbo

IPC分类号： C12P7/06

CPC分类号： C12P7/065 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1294 ,  C12N9/88 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

摘要翻译： 本发明提供了对重组宿主细胞中碳通量的操作以增加所需产物的形成。 本发明涉及经过遗传修饰的纤维素消化生物，以通过在中枢代谢的关键步骤重新定向碳通量以高产率生产乙醇。

公开(公告)号：US20140295516A1

公开(公告)日：2014-10-02

申请号：US14356510

申请日：2012-11-09

申请人： Mascoma Corporation

发明人： D. Aaron Argyros ,  Nicky Caiazza ,  Trisha F. Barrett ,  Anne K. Warner

IPC分类号： C12N9/90 ,  C12P7/10

CPC分类号： C12P7/10 ,  C07K14/38 ,  C07K14/385 ,  C07K14/39 ,  C12N1/16 ,  C12N9/1205 ,  C12N9/2402 ,  C12N9/90 ,  C12N15/52 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention provides a microorganism capable of fermenting arabmose to a desired product such as ethanol. In some embodiments, the organism is also capable of fermenting xylose. In some embodiments, the organism is capable of fermenting arabinose and xylose, and expresses one or more cellulases.

摘要翻译： 本发明提供能够将阿拉伯糖发酵成所需产物如乙醇的微生物。 在一些实施方案中，生物体还能够发酵木糖。 在一些实施方案中，生物体能够发酵阿拉伯糖和木糖，并表达一种或多种纤维素酶。

公开(公告)号：US20150024438A1

公开(公告)日：2015-01-22

申请号：US14188360

申请日：2014-02-24

申请人： Mascoma Corporation

发明人： Elena E. Brevnova ,  Vineet Rajgarhia ,  Mark Mellon ,  Anne Warner ,  John McBride ,  Chhayal Gandhi ,  Erin Wiswall

IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  C12P7/10

CPC分类号： C12N9/2437 ,  C12N15/81 ,  C12N15/815 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16

摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

摘要翻译： 本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。 纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。 纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。 在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。 因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

公开(公告)号：US20140308724A1

公开(公告)日：2014-10-16

申请号：US14178653

申请日：2014-02-12

申请人： Stellenbosch University ,  Mascoma Corporation

发明人： Elena BREVNOVA ,  John E. McBride ,  Erin Wiswall ,  Kevin S. Wenger ,  Nicky Caiazza ,  Heidi Hau ,  Aaron Argyros ,  Frank Agbogbo ,  Charles F. Rice ,  Trisha Barrett ,  John S. Bardsley ,  Abigail Foster ,  Anne K. Warner ,  Mark Mellon ,  Ryan Skinner ,  Indraneel Shikhare ,  Riaan Den Haan ,  Chhayal V. Gandhi ,  Alan Belcher ,  Vineet B. Rajgarhia ,  Allan C. Froehlich ,  Kristen M. Deleault ,  Emily Stonehouse ,  Shital A. Tripathi ,  Jennifer Gosselin ,  Yin-Ying Chiu ,  Haowen Xu

IPC分类号： C12P7/06 ,  C12N9/24

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

摘要翻译： 本发明涉及分泌全套或全套的酶的酵母菌株或菌株，以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素中的连接酶，半纤维素和 木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。 所得菌株或菌株可以同时水解淀粉和纤维素。 所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。 所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。 所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。 此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共培养并用于从生物质原料生产乙醇。

公开(公告)号：US20140322783A1

公开(公告)日：2014-10-30

申请号：US14361948

申请日：2012-11-30

申请人： Adam M. GUSS ,  Mascoma Corporation ,  Dartmouth College

发明人： Jonathan Lo ,  Adam M. Guss ,  Johannes P. Van Dijken ,  Arthur J. Shaw, IV ,  Daniel G. Olson ,  Christopher D. Herring

IPC分类号： C12P7/06 ,  C12N15/74

摘要： The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

摘要翻译： 本发明提供了对重组宿主细胞中辅因子使用的操作以增加所需产物的形成。 在一些实施方案中，本发明提供了包含一种或多种天然酶中的突变的重组微生物，使得它们的辅因子特异性以使得细胞中的总辅助因子在特定途径中平衡的方式改变，并且增加 细胞内的特定产物形成。 在一些实施方案中，内源性酶由具有来自不同物种的替代辅因子特异性的酶代替。

公开(公告)号：US20140256011A1

公开(公告)日：2014-09-11

申请号：US14075846

申请日：2013-11-08

申请人： Mascoma Corporation

发明人： Rintze Meindert ZELLE ,  Arthur J. Shaw, IV ,  Johannes Pieter Van Dijken

IPC分类号： C12N15/81 ,  C12P7/06

CPC分类号： C12P7/10 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/52 ,  C12N15/81 ,  C12P7/06 ,  C12Y101/01175 ,  C12Y101/01307 ,  C12Y102/0101 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention provides for novel metabolic pathways to detoxify biomass-derived acetate via metabolic conversion to ethanol, acetone, or isopropanol. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more first engineered metabolic pathways to achieve: (1) conversion of acetate to ethanol; (2) conversion of acetate to acetone; or (3) conversion of acetate to isopropanol; and one or more native and/or heterologous enzymes that function in one or more second engineered metabolic pathways to produce an electron donor used in the conversion of acetate to less inhibitory compounds; wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.

摘要翻译： 本发明提供了通过代谢转化为乙醇，丙酮或异丙醇将生物质衍生的乙酸盐解毒的新型代谢途径。 更具体地，本发明提供了一种重组微生物，其包含在一个或多个第一工程化代谢途径中起作用的一种或多种天然和/或异源酶，以实现：（1）将乙酸酯转化为乙醇; （2）乙酸酯转化为丙酮; 或（3）将乙酸酯转化成异丙醇; 和一种或多种在一种或多种第二工程化代谢途径中起作用的天然和/或异源酶，以产生用于将乙酸转化为较少抑制性化合物的电子给体; 其中所述一种或多种天然和/或异源酶被激活，上调或下调。