公开(公告)号：US20060263863A1

公开(公告)日：2006-11-23

申请号：US11489050

申请日：2006-07-18

申请人： Frederick Hahn ,  Adelheid Kuehnle

发明人： Frederick Hahn ,  Adelheid Kuehnle

IPC分类号： C12P17/00

CPC分类号： C12P9/00 ,  C12N9/90 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8214 ,  C12N15/8243

摘要： Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).

公开(公告)号：US20050241017A1

公开(公告)日：2005-10-27

申请号：US11053541

申请日：2005-02-08

申请人： Frederick Hahn ,  Adelheid Kuehnle

发明人： Frederick Hahn ,  Adelheid Kuehnle

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N15/29 ,  C12N15/31 ,  C12N15/52 ,  C12N15/61 ,  C12N15/82

CPC分类号： C12P9/00 ,  C12N9/90 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8214 ,  C12N15/8243

摘要： Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).

摘要翻译： 公开了甲羟戊酸和类异戊二烯生物合成途径的特定基因和无活性基因位点（假基因）的用途，以（1）增强转基因植物和微藻质体中异戊烯基二磷酸，二甲基烯丙基二磷酸和异戊二烯通路衍生产物的生物合成， （2）创建新的抗生素抗性转基因植物和微藻，（3）创建一个新的选择系统和/或靶向位点，用于介导遗传物质插入植物和微藻质体。 待使用的单独或以任何组合形式存在的特异性多核苷酸可从GeneBank公开获得并含有具有序列的开放阅读框，其表达将产生具有以下酶活性的活性蛋白：（a）乙酰乙酰辅酶A硫解酶（EC 2.3.1.9 ），（b）3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）合酶（EC 4.1.3.5），（c）HMG-CoA还原酶（EC 1.1.1.34），（d）甲羟戊酸激酶 （e）磷酸戊糖酸激酶（EC 2.7.4.2），（f）甲羟戊酸二磷酸脱羧酶（EC 4.1.1.33），（g）异戊烯基二磷酸（IPP）异构酶（EC 5.3.3.2）和（h） 合成酶（EC 2.5.1.32）。

公开(公告)号：US06706394B2

公开(公告)日：2004-03-16

申请号：US09847965

申请日：2001-05-02

申请人： Manfred R. Kuehnle ,  Adelheid Kuehnle

发明人： Manfred R. Kuehnle ,  Adelheid Kuehnle

IPC分类号： G11B566

CPC分类号： H01F1/143 ,  A61K9/5094 ,  A61K47/6923 ,  A61K48/0008 ,  C12N15/87 ,  H01F1/20 ,  Y10S428/90 ,  Y10T428/257 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2998

摘要： The subject invention related to methods and apparatuses for the manufacture of magnetizable carrier particles. In addition the subject invention pertains to particles having one or more of a variety of particle configurations and/or functional features. These geometric particle configurations and/or functional features such as delivering or removing a pay load can be tailored to achieve one or more desired missions. The subject invention also pertains to a method and apparatus for the delivery of particles to target materials, in order to accomplish one or more of a variety of missions. In a specific embodiment of the subject invention, acicular and other particles with a lengthwise dimension that are substantially uniform and homogenous in their geometry are manufactured and provided with magnetizations. In this way, predictable mechanical force responsivity can be achieved when these particles are subjected to an external magnetic field gradient.

摘要翻译： 本发明涉及制造可磁化载体颗粒的方法和装置。 此外，本发明涉及具有多种颗粒构型和/或功能特征中的一种或多种的颗粒。 可以调整这些几何颗粒配置和/或诸如递送或去除工作负载的功能特征以实现一个或多个期望的任务。 本发明还涉及用于将颗粒递送到目标材料的方法和装置，以便完成各种任务中的一种或多种。 在本发明的一个具体实施方案中，制造具有在其几何形状上基本均匀和均匀的纵向尺寸的针状和其它颗粒并提供磁化。 以这种方式，当这些颗粒经受外部磁场梯度时，可以实现可预测的机械力响应性。

公开(公告)号：US06617153B2

公开(公告)日：2003-09-09

申请号：US10005417

申请日：2001-12-05

申请人： Adelheid Kuehnle ,  Manfred R. Kuehnle

发明人： Adelheid Kuehnle ,  Manfred R. Kuehnle

IPC分类号： C12N1587

CPC分类号： C12M35/04 ,  C12M35/06

摘要： A method of delivery of reactive substances that are attached to magnetizable needle-like particles using a magneto-mechanical delivery device. The subject method and device can be utilized for the delivery of reactive or other substances, such as DNA via the penetration of a target body. Such penetration of a target or multiple targets can initiate the interaction between the material contained within the target site and the chemical substances delivered by the particles into the targets. In a preferred embodiment, the subject device is portable and does not require electrical power.