公开(公告)号：US4195125A

公开(公告)日：1980-03-25

申请号：US971526

申请日：1978-12-20

申请人： Adolf Wacker

发明人： Adolf Wacker

IPC分类号： C07K14/62 ,  A01N1/02

CPC分类号： C07K14/62

摘要： A process for obtaining insulin-producing cells by fusing islet cells derived from an animal or human pancreas with cells growing in suspension.

摘要翻译： 通过将源自动物或人胰腺的胰岛细胞与悬浮液中生长的细胞融合来获得胰岛素产生细胞的方法。

公开(公告)号：US4139611A

公开(公告)日：1979-02-13

申请号：US848109

申请日：1977-11-03

申请人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

发明人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

IPC分类号： C07K4/12 ,  C07K14/625 ,  A61K35/14 ,  A61K37/00 ,  A61K37/26

CPC分类号： C07K14/625 ,  C07K4/12 ,  Y10S530/829 ,  Y10S530/83

摘要： Organic peptide containing substances with insulin-like activity derived from blood or a blood component of a warm-blooded animal, which comprises the steps of pre-treating the blood or blood component by adjusting the pH thereof to a value of from 2 to 4 or of from 8 to 10, incubating the pre-treated blood at a temperature of from about 15 to about 40.degree. C for a period of at least four hours, cooling the incubated blood to a temperature of less than 10.degree. C, and recovering from the treated blood after neutralization organic peptide substances having a molecular weight of from about 1000 to about 5000, and an insulin-like activity of from about 14 to about 40 .mu.U/mg solid.

摘要翻译： 含有源自血液或温血动物血液成分的胰岛素样活性的含有有机肽的物质包括以下步骤：通过将其pH值调节至2至4或 8至10℃，将预处理血液在约15至约40℃的温度下孵育至少4小时，将孵育的血液冷却至低于10℃的温度，并从 处理后的血液中和有机肽物质的分子量为约1000至约5000，胰岛素样活性为约14至约40微米U / mg固体。

公开(公告)号：US4085204A

公开(公告)日：1978-04-18

申请号：US748956

申请日：1976-12-09

申请人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

发明人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

IPC分类号： A61K35/14 ,  A61K38/00 ,  A61K38/16 ,  A61K38/28 ,  A61P3/08 ,  A61P3/10 ,  A61P5/00 ,  C07K4/12 ,  C07K14/435 ,  C07K14/625 ,  A61K35/16 ,  A61K37/02

CPC分类号： C07K4/12 ,  C07K14/625 ,  Y10S530/829 ,  Y10S530/83

摘要： The invention concerns a novel process for obtaining insulin-like active substances from blood or a blood component of a warm-blooded animal, which comprises the steps of pre-treating the blood or blood component by adjusting the pH thereof to a value of from 2 to 4 or of from 8 to 10, incubating the pre-treated blood at a temperature of from about 15.degree. to about 40.degree. C for a period of at least four hours, cooling the incubated blood to a temperature of less than 10.degree. C, and recovering from the treated blood after neutralization organic peptide substances having a molecular weight of from about 1000 to about 5000, and an insulin-like activity of from about 14 to about 40 .mu.U/mg solid.

摘要翻译： 本发明涉及一种从温血动物血液或血液成分中获得胰岛素样活性物质的新方法，其包括以下步骤：通过将其pH值调节至2以预处理血液或血液成分 至4或8至10℃，将预处理血液在约15℃至约40℃的温度下孵育至少4小时，将培养的血液冷却至低于10℃的温度 并且在中和后的处理血液中回收具有约1000至约5000的分子量的有机肽物质和约14至约40μU/ mg固体的胰岛素样活性。