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    Purification of insulin
    1.
    发明授权
    Purification of insulin 有权
    胰岛素的纯化

    公开(公告)号：US09447163B2

    公开(公告)日：2016-09-20

    申请号：US13980969

    申请日：2012-02-01

    申请人： Joergen M. Mollerup ,  Soeren Soendergaarad Frederiksen

    发明人： Joergen M. Mollerup ,  Soeren Soendergaarad Frederiksen

    IPC分类号： C07K14/62 ,  C07K14/625

    CPC分类号： C07K14/62 ,  C07K14/625

    摘要： The present application discloses a chromatographic process for separating protein components of a protein-containing solution, the solution comprising an insulin peptide and one or more di- or polyvalent metal ions, the insulin peptide being capable of self-association and/or structural change in the presence of di- or polyvalent metal ions, the process comprising the steps of: a) applying the protein-containing solution to a column of a chromatographic solid phase material, wherein the loading of the insulin peptide is at least 6.0 g per liter of column volume (g/LCV); and b) eluting the insulin peptide from the solid phase material using an eluent having a pH of at the most 8.5; and collecting a pool of the insulin peptide corresponding to at least 75% by weight of the insulin peptide applied to the column in step (a).

    摘要翻译： 本申请公开了一种用于分离含蛋白质溶液的蛋白质组分的色谱方法，所述溶液包含胰岛素肽和一种或多种二价或多价金属离子，所述胰岛素肽能够自身结合和/或结构变化 二价或多价金属离子的存在，该方法包括以下步骤：a）将含蛋白质的溶液施加到色谱固相物质的柱上，其中胰岛素肽的载量为至少6.0g / 柱体积（g / LCV）; 和b）使用pH为至多8.5的洗脱液从固相物质洗脱胰岛素肽; 以及收集对应于步骤（a）中应用于所述柱的胰岛素肽的至少75重量％的胰岛素肽库。

    Purification of Insulin
    5.
    发明申请
    Purification of Insulin 有权
    胰岛素的纯化

    公开(公告)号：US20140073759A1

    公开(公告)日：2014-03-13

    申请号：US13980969

    申请日：2012-02-01

    申请人： Joergen M. Mollerup ,  Soeren Soendergaarad Frederiksen

    发明人： Joergen M. Mollerup ,  Soeren Soendergaarad Frederiksen

    IPC分类号： C07K14/62

    CPC分类号： C07K14/62 ,  C07K14/625

    摘要： The present application discloses a chromatographic process for separating protein components of a protein-containing solution, said solution comprising an insulin peptide and one or more di- or polyvalent metal ions, said insulin peptide being capable of self-association and/or structural change in the presence of di- or polyvalent metal ions, said process comprising the steps of: a) applying the protein-containing solution to a column of a chromatographic solid phase material, wherein the loading of the insulin peptide is at least 6.0 g per litre of column volume (g/Lcv); and b) eluting the insulin peptide from said solid phase material by means of an eluent having a pH of at the most 8.5; and collecting a pool of the insulin peptide corresponding to at least 75% by weight of the insulin peptide applied to the column in step (a).

    摘要翻译： 本申请公开了一种用于分离含蛋白质溶液的蛋白质组分的色谱方法，所述溶液包含胰岛素肽和一种或多种二价或多价金属离子，所述胰岛素肽能够自身结合和/或结构改变 二价或多价金属离子的存在，所述方法包括以下步骤：a）将含蛋白质的溶液施加到色谱固相材料的柱中，其中胰岛素肽的载量为至少6.0g / 柱体积（g / Lcv）; 和b）通过pH为至多8.5的洗脱液从所述固相物质洗脱胰岛素肽; 以及收集对应于步骤（a）中应用于所述柱的胰岛素肽的至少75重量％的胰岛素肽库。

    Process for recovering insulin
    6.
    发明授权
    Process for recovering insulin 失效
    胰岛素回收方法

    公开(公告)号：US4459226A

    公开(公告)日：1984-07-10

    申请号：US352725

    申请日：1982-02-26

    申请人： Denise L. Grimes ,  C. Stephen Hollinden

    发明人： Denise L. Grimes ,  C. Stephen Hollinden

    IPC分类号： C07K14/625 ,  C07C103/52

    CPC分类号： C07K14/625

    摘要： Insulin can be recovered for processing and re-use from an insulin-protamine complex by bringing the complex into contact with an aqueous medium having a pH of from about 2 to about 5 and containing an insulin dissociating or depolymerizing agent, a cation exchanger, and a salt at a concentration of from about 0.2 M to about 0.6 M.

    摘要翻译： 胰岛素可以通过使复合物与pH为约2至约5的水性介质接触并包含胰岛素解离或解聚剂，阳离子交换剂和 浓度为约0.2M至约0.6M的盐。

    Therapeutic insulin preparation and a process for the production of a stable insulin preparation with protracted effect
    7.
    发明授权
    Therapeutic insulin preparation and a process for the production of a stable insulin preparation with protracted effect 失效
    治疗性胰岛素制剂和用于产生具有延长作用的稳定胰岛素制剂的方法

    公开(公告)号：US4183849A

    公开(公告)日：1980-01-15

    申请号：US858916

    申请日：1977-12-08

    申请人： Bruno A. Hansen ,  Finn H. Andresen

    发明人： Bruno A. Hansen ,  Finn H. Andresen

    IPC分类号： A61K9/00 ,  A61K38/00 ,  A61K47/48 ,  C07K14/625 ,  A61K37/26

    CPC分类号： A61K9/0019 ,  A61K47/48184 ,  C07K14/625 ,  A61K38/00

    摘要： Novel stable insulin preparations with protracted action and reduced or no antigenicity are obtained by reacting insulin free of antigenic impurities with an organic compound having basic amino groups, preferably a basic polypeptide or a cleavage product of a basic polypeptide, such as protamine, in a buffered protein dissociating or protein depolymerizing aqueous medium. Examples of preferred protein dissociating or protein depolymerizing media are aqueous solutions containing urea, a lower alkanol dialkylformamide, acetamide, N-alkyl-acetamide or acetonitrile.

    摘要翻译： 通过使不含抗原杂质的胰岛素与具有碱性氨基的有机化合物，优选碱性多肽或碱性多肽如鱼精蛋白的切割产物在缓冲液中反应，获得具有延长作用和降低或无抗原性的新型稳定胰岛素制剂 蛋白质解离或蛋白质解聚水性介质。 优选的蛋白质解离或蛋白质解聚介质的实例是含有尿素，低级链烷醇二烷基甲酰胺，乙酰胺，N-烷基 - 乙酰胺或乙腈的水溶液。

    Process for purifying insulin
    8.
    发明授权
    Process for purifying insulin 失效
    胰岛素纯化方法

    公开(公告)号：US3907676A

    公开(公告)日：1975-09-23

    申请号：US36034073

    申请日：1973-05-14

    申请人： NOVO TERAPEUTISK LABOR AS

    发明人： JORGENSEN KLAVS HOLGER

    IPC分类号： C07K14/625 ,  B01D15/08 ,  C07C103/52 ,  C07G7/00

    CPC分类号： C07K14/625

    摘要： The present invention relates to a process of reducing the antigenicity of insulin recovered from pancreas glands of domestic mammals, particularly pork and bovine pancreas glands, and containing antigenic insulin-like substances with a molecular weight about 6,000 together with some antigenic proteins of pancreatic origin with a molecular weight above 6,000. The reduction in antigenicity is obtained by subjecting the insulin to column chromatography on an anion exchanger which is preferably strongly basic while using a water-containing monohydric aliphatic alcohol as eluent, and collecting the eluate fractions containing insulin free or essentially free of the impurities referred to. The purified insulin may be used in all kinds of insulin preparations for clinical use.

    摘要翻译： 本发明涉及一种降低从家畜哺乳动物，特别是猪和牛胰腺胰腺回收的胰岛素的抗原性的方法，并且含有分子量约为6,000的抗原性胰岛素样物质​​以及胰腺起源的一些抗原蛋白， 分子量高于6000。 抗原性的降低是通过在使用含水一元脂肪醇作为洗脱液的阴离子交换剂上进行柱层析，优选强碱性的，并收集含有不含或不含有所谓杂质的胰岛素的洗脱液级分 。 纯化的胰岛素可用于各种用于临床用途的胰岛素制剂。