-
公开(公告)号：US20240212844A1
公开(公告)日：2024-06-27
申请号：US18288017
申请日：2022-06-24
发明人： Xiang ZHU , Chenlu JIANG , Jiacheng ZHAO , Yibin QIN , Xiaqing MA , Cuie LU , Qiang GU , Weiwei ZHOU , Can ZHOU , Jiapei CAI , Zhusheng CHEN , Ming LI
摘要： A chronic pain Internet plus management platform is provided to perform file management for patients with chronic diseases by taking multi-dimension and multi-level information about patients as a starting point for chronic disease diagnosis, taking respective hospitals and home personal mobile devices as an information collecting media and integrating with an Internet chronic disease cloud database, thereby realizing AI-assisted screening, and intelligent analysis and prediction of health risks. Anew mode for chronic pain management is established by the new generation of information technology, such as mobile Internet, cloud computing and big data; meanwhile, self-management of the patient is assisted by the mode for chronic pain management to improve prognosis of chronic pain.
-
公开(公告)号：US20220151997A1
公开(公告)日：2022-05-19
申请号：US17435832
申请日：2020-03-24
发明人： Mingbing XIAO , Yifei JI , Zhifeng GU , Wei SHI , Jie JI , Yihui FAN
IPC分类号： A61K31/426 , G01N1/30 , G01N33/50 , A61P35/00
摘要： The invention provides use of epalrestat in preparation of pancreatic cancer drugs. The pancreatic cancer drugs are used for inhibiting secretion of exosomes from pancreatic cancer cells. The invention also provides a method for verifying the inhibition effect of epalrestat on secretion of exosomes from pancreatic cancer cells. The method includes steps of: extracting cell supernatant exosomes by using a low-temperature ultracentrifugation method; lysing the collected exosomes, then using a BCA kit to quantify the resulting exosomal protein, and using the measured amount of the protein to reflect the amount of the exosomes; using a transmission electron microscope to verify a double-layer lipid membrane wrapped cup-shaped structure of the exosomes; and detecting exosome protein concentration by protein polyacrylamide gel electrophoresis with Coomassie brilliant blue. The invention provides a novel use of epalrestat, namely the inhibition of exosome secretion. The epalrestat has great application potential in clinical tumor treatment.
-
公开(公告)号：US20240018222A1
公开(公告)日：2024-01-18
申请号：US18256221
申请日：2022-01-04
发明人： Jianfei HUANG , Bing LU , Pingping SUN , Xiaojing ZHANG
IPC分类号： C07K16/18 , G01N33/574
CPC分类号： C07K16/18 , G01N33/57446 , G01N33/57415 , G01N33/57407 , C07K2317/76 , C07K2317/35
摘要： A monoclonal antibody inhibiting an SMIM15 and application thereof; the antibody is secreted by a hybridoma cell strain 1M5. A preparation method includes: preparing mouse immunogen and an antigen for detection; preparing the hybridoma cell strain 1M5 against an SMIM15; screening an SMIM15 positive monoclonal antibody: identifying a subtype of the monoclonal antibody; detecting specificity of the SMIM15 positive monoclonal antibody; producing and purifying the monoclonal antibody in a BALB/c mouse; detecting purity of the antibody by SDS-PAGE; and performing function identification and application on the SMIM15 monoclonal antibody with WB, IHC and ELISA. According to the present application, the antibody secreted by the hybridoma cell strain 1M5 is an immunoglobulin, which is non-toxic, and can be used for Western blot and immunohistochemistry and used as an active ingredient to be made into a reagent or drug for diagnosis and treatment of SMIM15-related diseases.
-
公开(公告)号：US20220187302A1
公开(公告)日：2022-06-16
申请号：US17593787
申请日：2020-03-24
发明人： Mingbing XIAO , ZhaoXiu LIU , Wei SHI , Zhifeng GU , Minxue XU , Zhonghua TAN
IPC分类号： G01N33/574 , G01N33/53
摘要： The present invention provides a detection device of dot immunoblotting detection, including a negative pressure suction device, a hole plate, a hose for connecting the hole plate with the negative pressure suction device, and a nitrocellulose membrane in tight fit with the upper end surface of the hole plate. The present invention further provides a detection method of dot immunoblotting detection, including preparation, sample injection, blocking, incubation of a primary antibody, incubation of a secondary antibody, development and analysis. The detection method performs negative pressure suction through the negative pressure suction device, which is favorable for concentrating samples during sample injection and avoiding the influence, caused by cross contamination after diffusion of the samples, on an experimental result, and the experimental result is more accurate.
-
-
-
搜索结果
4 条记录 (耗时 0.012 秒)
