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1.发明申请Chip Production, Hybridization and Data Interpretation for Antibody and Protein Microarrays 有权芯片生产，抗体和蛋白质微阵列的杂交和数据解释公开(公告)号：US20080058215A1
公开(公告)日：2008-03-06
申请号：US10593412
申请日：2005-03-18
CPC分类号： G01N33/54366 , G01N33/54306 , G01N33/573 , G01N2333/90245
摘要： An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.
摘要翻译： 一种包含底物，纯化免疫球蛋白的单克隆抗体和多克隆抗体的抗体微阵列筛选，其中所述抗体被点在基底上的预定位置，以及未加工用于免疫球蛋白分离的流体（例如抗血清，腹水或杂交瘤培养基） ，其中未处理的流体被点在基板上的预定位置上。 公开了含有密切相关的细胞色素P450的药物代谢酶抗体微阵列的生产。 公开了制造抗体微阵列的方法，用于抗体微阵列的内部对照分子，确定IgG的最佳点样浓度的方法以及通过微阵列分析增加可检测信号的方法。
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2.发明授权Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms 失效用于鉴定和比较真核生物体中表达基因（mRNA）的组合物和系统公开(公告)号：US06955876B2
公开(公告)日：2005-10-18
申请号：US10002536
申请日：2001-11-01
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q2531/143 , C12Q2525/173 , C12Q2525/143
摘要： The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The invention comprises, without limitation, one or more mRNA specific identimers for use in reverse transcription that themselves comprise an oligo-T nucleotide sequence (at the 5′ end) linked to a nucleotide sequence VNx (at the 3′ end) where the V nucleotide immediately adjacent to the oligo-T segment is not a T.
摘要翻译： 本发明包括用于鉴定和比较给定体内或体外RNA样品中的表达基因的组合物和系统，以及在两个或多个样品之间的mRNA表达的相对差异。 此外，本发明包括鉴定新基因的组合物和系统。 本发明包括但不限于用于逆转录的一个或多个mRNA特异性鉴定子，其本身包含与核苷酸序列VNx（在3'末端）连接的寡核苷酸-T-核苷酸序列（在5'末端），其中V 与oligo-T片段紧邻的核苷酸不是T.
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3.发明授权Chip production, hybridization and data interpretation for antibody and protein microarrays 有权针对抗体和蛋白质微阵列的芯片生产，杂交和数据解释公开(公告)号：US08765641B2
公开(公告)日：2014-07-01
申请号：US10593412
申请日：2005-03-18
IPC分类号： C40B30/04 , G01N33/563
CPC分类号： G01N33/54366 , G01N33/54306 , G01N33/573 , G01N2333/90245
摘要： An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.
摘要翻译： 一种包含底物，纯化免疫球蛋白的单克隆抗体和多克隆抗体的抗体微阵列筛选，其中所述抗体被点在基底上的预定位置，以及未加工用于免疫球蛋白分离的流体（例如抗血清，腹水或杂交瘤培养基） ，其中未处理的流体被点在基板上的预定位置上。 公开了含有密切相关的细胞色素P450的药物代谢酶抗体微阵列的生产。 公开了制造抗体微阵列的方法，用于抗体微阵列的内部对照分子，确定IgG的最佳点样浓度的方法以及通过微阵列分析增加可检测信号的方法。
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