公开(公告)号：US11828755B2

公开(公告)日：2023-11-28

申请号：US16307125

申请日：2017-06-07

Applicant: The Regents of the University of California

Inventor： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  Daniel William Bradbury ,  Shin Ting Sherine Frieda Cheung

IPC: G01N33/543 ,  C12Q1/6844 ,  C12Q1/6813

CPC classification number: G01N33/54388 ,  C12Q1/6813 ,  C12Q1/6844 ,  G01N33/543 ,  G01N33/54306 ,  C12Q1/6813 ,  C12Q2563/159 ,  C12Q2565/625 ,  C12Q1/6844 ,  C12Q2521/513 ,  C12Q2531/119 ,  C12Q2531/143 ,  C12Q2563/159

Abstract: In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.

公开(公告)号：US11746372B2

公开(公告)日：2023-09-05

申请号：US16768802

申请日：2018-12-03

Applicant: GoDX, inc. ,  The United States of America, as Represented by the Secretary, Department of Health and Human Services

Inventor： Chang Hee Kim ,  Lichen Xiang ,  Wendy A. Henderson ,  Xiao Jiang

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6865

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6865 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2531/143 ,  C12Q2563/155

Abstract: Disclosed herein are method for separating, amplifying, or detecting a nucleic acid from a sample may comprise contacting a sample lysate with a plurality of buoyant, inorganic, nucleic-acid-capture microspheres. The nucleic-acid-capture microspheres may comprise unicellular hollow microspheres having a diameter between 5 and 300 μm and/or a true particle density between 0.05 and 0.60 grams/cm3. The microspheres may comprise hollow soda-lime-borosilicate microspheres. In some embodiments, the microspheres comprises hollow soda-lime-borosilicate microspheres surrounded by an amorphous silica shell. Also disclosed are kits for performing the methods.

公开(公告)号：US20170368549A1

公开(公告)日：2017-12-28

申请号：US15546511

申请日：2015-01-30

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Manish Giri ,  Chantelle Elizabeth Domingue ,  Nicholas Matthew Cooper McGuinness ,  Jeremy Sells ,  Sirena Lu ,  Melinda M. Valencia

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/502753 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/027 ,  B01L2300/0636 ,  B01L2300/0663 ,  B01L2300/088 ,  B01L2300/1827 ,  B01L2400/046 ,  C12Q1/005 ,  C12Q1/6804 ,  G01N15/0656 ,  G01N33/54373 ,  G01N33/5438 ,  G01N2015/0065 ,  G01N2015/0687 ,  C12Q2531/113 ,  C12Q2531/143 ,  C12Q2563/131 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A microfluidic diagnostic chip may comprise a microfluidic channel, a functionalizable enzymatic sensor in the microfluidic channel, the functionalizable enzymatic sensor comprising a binding surface to bind with a biomarker in a fluid, and a microfluidic pump to pass the fluid over the binding surface. A microfluidic device may comprise a number of pumps to pump a fluid though the number of microfluidic channels and a number of microfluidic channels comprising at least one sensor to detect a change in a chemical characteristic of the fluid in response to presence of the fluid on the sensor

公开(公告)号：US20160348162A1

公开(公告)日：2016-12-01

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20160257984A1

公开(公告)日：2016-09-08

申请号：US14990276

申请日：2016-01-07

Applicant: 10X Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34

CPC classification number: C12P19/34 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12Q1/6806 ,  C40B20/04 ,  C12Q2525/121 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2535/122 ,  C12Q2563/149

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Abstract translation: 本公开提供了制备测序文库的方法，包括提供模板核酸序列，dNTP，dUTP，引物，聚合酶，dUTP切割酶和包含寡核苷酸衔接子序列片段的多个珠粒的步骤; 用聚合酶，dNTP，dUTP和随机六聚体扩增模板核酸，以提供包括偶尔的dUTP的互补核酸序列; 并用dUTP切割酶切除掺入的dUTP以在互补核酸序列中提供缺口以提供测序文库。

公开(公告)号：US09169512B2

公开(公告)日：2015-10-27

申请号：US13380018

申请日：2010-07-01

Applicant: Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

Abstract translation: 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

公开(公告)号：US08728765B2

公开(公告)日：2014-05-20

申请号：US13397416

申请日：2012-02-15

Applicant: Jesus Ching ,  Ronald Chang ,  Douglas Dority ,  Jian Ping Zhang ,  James Jian Quan Wang ,  Wendy Wong ,  Kendra Lara Paul ,  Reuel Van Atta

Inventor： Jesus Ching ,  Ronald Chang ,  Douglas Dority ,  Jian Ping Zhang ,  James Jian Quan Wang ,  Wendy Wong ,  Kendra Lara Paul ,  Reuel Van Atta

IPC: C12P19/34

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

Abstract translation: 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。

公开(公告)号：US08642268B2

公开(公告)日：2014-02-04

申请号：US13460341

申请日：2012-04-30

Applicant: Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

IPC: C12Q1/68 ,  C12P19/34 ,  C07H19/00

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

Abstract translation: 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。

公开(公告)号：US20090226885A1

公开(公告)日：2009-09-10

申请号：US10559949

申请日：2004-03-08

Applicant: P.T.G Sillekens ,  Marlieke Overdijk ,  Saskia van de Laar

Inventor： P.T.G Sillekens ,  Marlieke Overdijk ,  Saskia van de Laar

IPC: C12Q1/70 ,  C07H21/04 ,  C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q2531/113 ,  C12Q2531/143 ,  C12Q2561/101

Abstract: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with a novel human coronavirus causing Severe Acute Respiratory Syndrome (SARS). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of SARS nucleic acid. The oligonucleotide sequences provided with the present invention are located in the replicase gene, the nucleocapsid gene and the 3′ end non-coding region of the SARS Coronavirus genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of SARS Coronavirus can be obtained. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.

Abstract translation: 本发明涉及可用于病毒诊断领域的核酸序列，更具体地说，涉及引起严重急性呼吸综合征（SARS）的新型人冠状病毒感染的诊断。 本发明提供了可用作扩增和检测SARS核酸的引物和探针的核苷酸序列。 本发明提供的寡核苷酸序列位于SARS冠状病毒基因组的复制酶基因，核衣壳基因和3'端非编码区。 已经发现，通过将本发明的序列用于扩增和检测核酸的方法，可以获得SARS的敏感和特异性检测冠状病毒。 根据本发明的寡核苷酸序列特别可用于扩增核酸的方法。

公开(公告)号：US20080305482A1

公开(公告)日：2008-12-11

申请号：US11962072

申请日：2007-12-20

Applicant: Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

Abstract translation: 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。