公开(公告)号：US6049380A

公开(公告)日：2000-04-11

申请号：US169025

申请日：1998-10-09

申请人： Peter M. Goodwin ,  James H. Jett ,  Richard A. Keller ,  Alan K. Van Orden ,  Nicholas P. Machara

发明人： Peter M. Goodwin ,  James H. Jett ,  Richard A. Keller ,  Alan K. Van Orden ,  Nicholas P. Machara

IPC分类号： G01N21/64 ,  F21V9/16 ,  G01J3/30

CPC分类号： G01N21/6428 ,  G01N21/6408

摘要： Single fluorescent molecules in a flowing sample stream are distinguished and identified using only a single laser excitation wavelength. A sample stream is formed containing a dilute mixture of single molecule fluorophores, wherein each one of the fluorophores is serially ordered in the sample stream. The sample stream is illuminated with s single excitation wavelength laser effective to excite each fluorophore one at a time. Fluorescence emission photons from each said fluorophore are detected. A burst size is determined for each fluorophore to identify each fluorophore. A pulsed laser may be used, where burst size and an intra-burst fluorescence decay rate for each fluorophore are determined simultaneously from the detected fluorescence emission photons. The burst size and the decay rate are correlated to identify each fluorophore.

摘要翻译： 流动样品流中的单个荧光分子仅使用单个激光激发波长来识别和识别。 形成包含单分子荧光团的稀混合物的样品流，其中每个荧光团在样品流中连续排列。 用单个激发波长激光器照射样品流，有效地一次激发每个荧光团。 检测来自每个所述荧光团的荧光发射光子。 确定每个荧光团的突发尺寸以识别每个荧光团。 可以使用脉冲激光，其中从检测到的荧光发射光子同时测定每个荧光团的突发尺寸和爆发内荧光衰减速率。 脉冲串大小和衰减速率相关，以识别每个荧光团。