公开(公告)号：US20070077653A1

公开(公告)日：2007-04-05

申请号：US10570125

申请日：2004-09-03

申请人： Albert Banes ,  Jie QI

发明人： Albert Banes ,  Jie QI

IPC分类号： C12N5/08

CPC分类号： C12N5/066 ,  C12N2501/23 ,  C12N2533/54

摘要： The present invention provides methods for manipulating the intrinsic strain of cells by treating tissue engineered constructs or native tissue with compounds which affect the intrinsic strain setpoint of the cells in order to modulate matrix synthesis, secretion, organization and/or remodeling so that the tissues withstand in vivo mechanical forces and have the structural characteristics of host tissue which has been permanently altered by injury, atrophy or disease. The compounds include binding site peptides, ATP, UTP and related analogues, IL-1&bgr; TGF-&agr; cytochalasin D, hyaluronic acid, nocodazole and others. Also provided are methods for applying a mechanical external strain to the tissues, as well as methods for modulating the expression of cytoskeletal genes that transcribe cytoskeletal proteins which regulate a cell's intrinsic strain setpoint.

摘要翻译： 本发明提供了通过用影响细胞的固有应变设定点的化合物处理组织工程化构建体或天然组织来调节细胞内在应变以调节基质合成，分泌，组织和/或重塑以使组织耐受 体内机械力并具有被损伤，萎缩或疾病永久改变的宿主组织的结构特征。 化合物包括结合位点肽，ATP，UTP和相关类似物，IL-1βTGF-α细胞松弛素D，透明质酸，诺考达唑等。 还提供了将机械外部应变施加到组织的方法，以及用于调节转录调节细胞内在应变设定点的细胞骨架蛋白的细胞骨架基因的表达的方法。

公开(公告)号：US20060121513A1

公开(公告)日：2006-06-08

申请号：US11293891

申请日：2005-12-02

申请人： Albert Banes ,  Jie Qi

发明人： Albert Banes ,  Jie Qi

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705 ,  C12N15/87

CPC分类号： C07K14/4716 ,  A61K48/00 ,  C07K2319/09 ,  Y10S530/841

摘要： The present provides nuclear localization signaling (NLS) sequences derived from titin, comprised of amino acids 181-220: SVGRATSTAE LLVQGEEEVP AKKTKTIVST AQISESRQTR and fragments thereof, such as amino acids 193-208: VQGEEEVP AKKTKTIV; amino acids 199-208: VPAKKTKTIV; and amino acids 200-206: PAKKTKT. The NLS sequences can be linked to agents, such as peptides, proteins or nucleotides, for transporting the agents into the nucleus of cells, and the NLS-agent complex can be further linked to antibodies or ligands for specific binding to cells. Also provided is a method for constructing cDNAs comprising combining a NLS sequence with a nucleic acid sequence for a target protein for expression and entry of the target protein into the nucleus of cells, which then can perform specific functions therein.

公开(公告)号：US20090203547A1

公开(公告)日：2009-08-13

申请号：US11884496

申请日：2006-02-21

申请人： Albert Banes ,  Jie Qi ,  Donald K. Bynum ,  Beverly Koller ,  Jeffrey Thompson ,  Ann Fox ,  Allison Nation

发明人： Albert Banes ,  Jie Qi ,  Donald K. Bynum ,  Beverly Koller ,  Jeffrey Thompson ,  Ann Fox ,  Allison Nation

IPC分类号： C40B40/06 ,  C12Q1/68

CPC分类号： G01N33/6887 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2800/10

摘要： Differences in gene expression between connective tissue cells (e.g., tendon cells) and other closely related cell types are disclosed. Also disclosed are expression profiles between tendon cells under different genetic and environmental influences. The presently disclosed expression profiles are useful as diagnostic markers as well as markers that can be used to monitor disease states, disease progression, injury repair, drug toxicity, drug efficacy, and drug metabolism.

摘要翻译： 公开了结缔组织细胞（例如肌腱细胞）和其它紧密相关的细胞类型之间的基因表达差异。 还公开了不同遗传和环境影响下肌腱细胞之间的表达谱。 目前公开的表达谱可用作诊断标记物以及可用于监测疾病状态，疾病进展，损伤修复，药物毒性，药物功效和药物代谢的标记物。

公开(公告)号：US20060134779A1

公开(公告)日：2006-06-22

申请号：US11076425

申请日：2005-03-09

申请人： Albert Banes ,  Jie Qi

发明人： Albert Banes ,  Jie Qi

IPC分类号： A61H1/00 ,  C12N5/08

CPC分类号： C12N5/066 ,  C12N2527/00 ,  C12N2533/54

摘要： The present invention provides methods for manipulating the intrinsic strain of cells by treating tissue engineered constructs or native tissue with compounds which affect the intrinsic strain setpoint of the cells in order to modulate matrix synthesis, secretion, organization and/or remodeling so that the tissues withstand in vivo mechanical forces and have the structural characteristics of host tissue which has been permanently altered by injury, atrophy or disease. The compounds include binding site peptides, ATP, UTP and related analogues, IL-1β, TGF-α, cytochalasin D, hyaluronic acid, nocodazole and others. Also provided are methods for applying a mechanical external strain to the tissues, as well as methods for modulating the expression of cytoskeletal genes that transcribe cytoskeletal proteins which regulate a cell's intrinsic strain setpoint.

摘要翻译： 本发明提供了通过用影响细胞的固有应变设定点的化合物处理组织工程化构建体或天然组织来调节细胞内在应变以调节基质合成，分泌，组织和/或重塑以使组织耐受 体内机械力并具有被损伤，萎缩或疾病永久改变的宿主组织的结构特征。 化合物包括结合位点肽，ATP，UTP和相关类似物，IL-1β，TGF-α，细胞松弛素D，透明质酸，诺考达唑等。 还提供了将机械外部应变施加到组织的方法，以及用于调节转录调节细胞内在应变设定点的细胞骨架蛋白的细胞骨架基因的表达的方法。

公开(公告)号：US20070225597A1

公开(公告)日：2007-09-27

申请号：US10576182

申请日：2004-10-22

申请人： Albert Banes ,  Melissa Maloney

发明人： Albert Banes ,  Melissa Maloney

IPC分类号： A61B5/00

CPC分类号： G06T7/0012 ,  G06T2207/30024

摘要： Disclosed is a tissue engineered construct analytical imaging system (10) for use with culture wells (12) having tissue engineered constructs therein, which are positionable in an incubator apparatus (16) or other enclosed environment. The system (10) includes an imaging device (18) in operational communication with the enclosed environment for obtaining data reflective of a well area of interest in the culture well (12), without the removal of the culture well (12) from the enclosed environment. A computer controller (20) can receive data from the imaging device (18), analyze the data and determine desired parameters within the well area of interest and/or output data reflective of the results of the analysis. A computer-implemented method of obtaining and analyzing images of tissue engineered constructs is also disclosed.

摘要翻译： 公开了一种与其中具有组织工程化构建体的培养孔（12）一起使用的组织工程构建体分析成像系统（10），其可定位在培养箱装置（16）或其它封闭环境中。 系统（10）包括与封闭环境操作通信的成像装置（18），用于获取反映培养井（12）中感兴趣的井区域的数据，而不用从封闭的环境中移走培养井（12） 环境。 计算机控制器（20）可以从成像设备（18）接收数据，分析数据并确定感兴趣的区域内的期望参数和/或反映分析结果的输出数据。 还公开了一种获得和分析组织工程构建体图像的计算机实现方法。