公开(公告)号：US20120172316A1

公开(公告)日：2012-07-05

申请号：US13378605

申请日：2010-06-16

申请人： Paolo Morazzoni ,  Antonella Riva ,  Cesare Ponzone ,  Davide Berlanda ,  Marcello Duranti ,  Alessandro Consonni

发明人： Paolo Morazzoni ,  Antonella Riva ,  Cesare Ponzone ,  Davide Berlanda ,  Marcello Duranti ,  Alessandro Consonni

IPC分类号： A61K38/16 ,  C12P21/06 ,  C07K14/415

CPC分类号： C07K14/415

摘要： A truncated form of α′ chain (eα′), the soybean 7S globulin, active in controlling the cholesterol and triglyceride homeostasis in in vitro and in vivo models, was cloned and expressed in the yeast Pichia pastoris. The recombinant polypeptide spanned 142 amino acid residues from the N-terminal side and included the N-terminal extension region of the soybean alpha′ subunit. The eα′ polypeptide was purified by conventional biochemical techniques and its potential to modulate the activity of the LDL-receptor was evaluated in a human hepatoma cell line (Hep G2) by monitoring the uptake and degradation of labeled LDL.

摘要翻译： 在酵母巴斯德毕赤酵母中克隆并表达了在体外和体内模型中有效控制胆固醇和甘油三酯体内平衡的α'链（eα'）的截短形式的大豆7S球蛋白。 重组多肽跨越了N末端侧的142个氨基酸残基并且包括大豆α'亚基的N末端延伸区。 通过常规生物化学技术纯化eα'多肽，并通过监测标记的LDL的摄取和降解，在人肝癌细胞系（Hep G2）中评价其调节LDL-受体活性的潜力。

公开(公告)号：US08569455B2

公开(公告)日：2013-10-29

申请号：US13378605

申请日：2010-06-16

申请人： Paolo Morazzoni ,  Antonella Riva ,  Cesare Ponzone ,  Davide Berlanda ,  Marcello Duranti ,  Alessandro Consonni

发明人： Paolo Morazzoni ,  Antonella Riva ,  Cesare Ponzone ,  Davide Berlanda ,  Marcello Duranti ,  Alessandro Consonni

IPC分类号： C07K14/415 ,  C12P21/04 ,  A61K38/16

CPC分类号： C07K14/415

摘要： A truncated form of α′ chain (eα′), the soybean 7S globulin, active in controlling the cholesterol and triglyceride homeostasis in in vitro and in vivo models, was cloned and expressed in the yeast Pichia pastoris. The recombinant polypeptide spanned 142 amino acid residues from the N-terminal side and included the N-terminal extension region of the soybean alpha′ subunit. The eα′ polypeptide was purified by conventional biochemical techniques and its potential to modulate the activity of the LDL-receptor was evaluated in a human hepatoma cell line (Hep G2) by monitoring the uptake and degradation of labeled LDL.

摘要翻译： 在酵母巴斯德毕赤酵母中克隆并表达了在体外和体内模型中有效控制胆固醇和甘油三酯体内平衡的α'链（ealpha'）的截短形式，大豆7S球蛋白。 重组多肽跨越了N末端侧的142个氨基酸残基并且包括大豆α'亚基的N末端延伸区。 通过常规生物化学技术纯化ealpha多肽，并通过监测标记的LDL的摄取和降解，在人肝癌细胞系（Hep G2）中评价其调节LDL-受体活性的潜力。