公开(公告)号：US20060240423A1

公开(公告)日：2006-10-26

申请号：US10525708

申请日：2003-08-20

申请人： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jurgen Eck

发明人： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jurgen Eck

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12N15/1003

摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

摘要翻译： 本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子 尺寸。 此外，本发明涉及用于分离核酸分子的方法，包括将样品施用于本发明的装置，其中所述核酸分子优选代表给定栖息地的元素的一部分。 此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。